Messenger RNAs in corn pollen and protein synthesis during germination and pollen tube growth

Mascarenhas, N. T.; Bashe, David M.; Eisenberg, Arthur J.; Willing, R. P.; Xiao, Chong-Ming; Mascarenhas, Joseph P.

doi:10.1007/bf00267885

Cited by 67 publications

(37 citation statements)

References 12 publications

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“…Molecular studies identifying anther-and pollen-specific genes support the notion that the construction of a functional male gametophyte requires a rather large pool of such genes (Kamalay and Goldberg, 1980;Willing and Mascarenhas, 1984;McCormick, 1991). This is not so surprising given the specialized structures, mechanisms for rapid growth, and cell-cell communication systems during pollen-pistil interactions that have evolved to allow for both the survival of the gametophyte free of the sporophyte and the efficient delivery of sperm to the embryo sac.…”

Section: Introductionmentioning

confidence: 94%

“…The pollen grain accumulates starch granules until the grain is entirely engorged ( Figure 2H). It appears that some mRNAs synthesized at this time may be stored for translation during germination (Mascarenhas et al, 1984;Mascarenhas, 1990). The pollen dehydrates, reducing the water content to 40 to dehydration of the pollen grain after its release from the anther may cause domains of the vegetative cell plasma membrane to enter an unstable gel/liquid crystal state (Kerhoas et al, 1987).…”

Section: Overview Of Pollen Developmentmentioning

confidence: 99%

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The remarkable biology of pollen.

Bedinger

1992

Plant Cell

249

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Section: Introductionmentioning

confidence: 94%

Section: Overview Of Pollen Developmentmentioning

confidence: 99%

The remarkable biology of pollen.

Bedinger

1992

Plant Cell

249

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“…Such changes could be due to either new transcriptional events or to alterations in the translational capacity of preexisting messages in the microspore. It is known that pollen contains stored mRNAs that can be activated during pollen tube germination (12).…”

Section: Changes In Steady-state Protein Populations Occur At the Larmentioning

confidence: 99%

Developmental Staging of Maize Microspores Reveals a Transition in Developing Microspore Proteins

Bedinger¹,

Edgerton²

1990

Plant Physiol.

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A method for the preparation of developmentally staged microspores and young pollen from maize (Zea mays) has been devised. The preparations are of sufficient purity and quantity for biochemical analysis, including the analysis of steady-state protein and RNA populations associated with each stage. A major transition in protein populations occurs during the developmental period that encompasses microspore mitosis, the asymmetric nuclear division producing the vegetative and generative nuclei. Several differences between early and late stage proteins can be detected by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two-dimensional gel electrophoresis of proteins reveals that over half of the steady-state proteins differ between the younger and older stages, either quantitative or qualitative. One protein that increases in relative abundance about fourfold is actin. In vitro translation of RNA isolated from staged microspores demonstrates changes in microspore gene expression during the same developmental period.acious remnants of the tapetal cells are deposited on the surface of the pollen grain, which desiccates and is shed from the anther upon dehiscence.The study of pollen development provides an opportunity to examine the gametophyte generation of higher plants. While recent studies have suggested that multiple molecular events are involved in the progression of microspores through the pollen developmental pathway (4,(23)(24)(25), the scope of these events was unknown. The work presented here provides a method for the preparation of developmentally staged microspores and young pollen. These preparations are of sufficient purity and quantity to allow the characterization of protein and RNA populations associated with each stage. A major transition in steady-state protein populations has been observed between the large vacuole and starch-filling stages of microsporogenesis. MATERIALS AND METHODS MaterialsMicrosporogenesis in higher plants has been well described at the genetic and cytological levels (1,2,9,11,20). The maize microspore developmental pathway can be briefly summarized as follows (1,3,6,21)

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“…Further treatment of the extracts for two-dimensional electrophoresis and the conditions of electrophoresis were as described by Mascarenhas et al (19). All gels to be compared were run together on an electrophoresis apparatus that allowed the simultaneous electrophoresis of four samples.…”

mentioning

confidence: 99%

High Temperature-Induced Thermotolerance in Pollen Tubes of Tradescantia and Heat-Shock Proteins

Xiao¹,

Mascarenhas²

1985

Plant Physiol.

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Growing pollen tubes of Tradescantia paludosa are protected from inhibition of growth at 41°C by a prior exposure to gradually increasing temperatures. Heat shock proteins (hsps) are not synthesized by pollen tubes as determined by labeling with [3SJmethionine and two-dimensional gel electrophoresis, during either a heat shock at 41°C or a gradual temperature increase to 41°C. A comparison after two-dimensional electrophoresis of silver-stained spots and radioactive spots after autoradiography of an extract of ungerminated pollen mixed with a trace amount of I35Slmethionine-labeled vegetative tissue heat shocked at 41°C to act as a hsps marker, indicates that the majority, if not all, of the major hsps are not present in the pollen grain at anthesis. The type of thermotolerance seen with pollen tubes can thus be achieved without the presence or the new synthesis of the hsps.

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Messenger RNAs in corn pollen and protein synthesis during germination and pollen tube growth

Cited by 67 publications

References 12 publications

The remarkable biology of pollen.

The remarkable biology of pollen.

Developmental Staging of Maize Microspores Reveals a Transition in Developing Microspore Proteins

High Temperature-Induced Thermotolerance in Pollen Tubes of Tradescantia and Heat-Shock Proteins

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