Meta-topolin-mediated regeneration and accumulation of phenolic acids in the critically endangered medicinal plant Crinum malabaricum (Amaryllidaceae): A potent source of galanthamine

Chahal, Swati; Kaur, Harmeet; Lekhak, Manoj M.; Shekhawat, Mahipal S.; Goutam, Umesh; Singh, Sachin Kumar; Ochatt, Sergio; Kumar, Vijay

doi:10.1016/j.sajb.2022.01.016

Cited by 15 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BAP is a cytokinin used to stimulate the biosynthesis of rosmarinic acid and salvianolic acid in in vitro culture of Dracocephalum forrestii 60 . While m T, applied at lower concentrations (2.5 and 5 µM) similar to the present study, has been shown to contribute to an increase in the content of phenolic acids (chlorogenic acid, ferulic acid, p -coumaric acid and sinapic acid) in in vitro cultures of Crinum malabaricum 30 .…”

Section: Discussionsupporting

confidence: 75%

“…However, in recent studies, m T has been applied very frequently as an alternative to conventional cytokinins for the multiplications of shoots: Salvia sclarea 40 , Allamanda cathartica 29 , Oxystelma esculentum 31 , Rheum sp. ‘Malinowy’ 41 , Crinum malabaricum 30 , Crinum brachynema 42 and Caralluma umbellate 44 . Moreover, research on Crinum brachynema 42 and Aloe polyphylla 45 , similar to the presented results, showed that the 5 µM of m T is more favourable for the multiplication of shoots than higher concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…A natural cytokinin, m T has been recognised as an excellent elicitor of secondary metabolite biosynthesis, e.g. galanthamine, phenolic acids and flavonoids, but it has never been tested in stevia cultures 28 – 30 . However, a beneficial effect of m T has been demonstrated for improved multiplication rate and in vitro plant quality 28 , 31 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

Ptak,

Szewczyk,

Simlat

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Stevia rebaudiana Bertoni possesses various medicinal and food industrial applications. This study is the first to explore the effect of the cytokinins meta-Topolin (mT; 6-(3-hydroxybenzylamino) purine), zeatin, kinetin, and BAP (6-benzylaminopurine) at concentrations of 0 (control), 5, 10, and 15 µM on shoot multiplication, as well as stevioside, rebaudioside A, phenolic acid, and flavonoid content in bioreactor cultures. The highest number of shoots (23.4 per explant) was obtained in the medium containing 5 μM of mT. However, 15 μM of mT was superior for fresh biomass production and dry biomass accumulation. Reversed-phase (RP)-HPLC analysis showed a beneficial effect of 5 μM mT on stevioside (11.43 mg/g dry weight [DW]) and rebaudioside A (10.74 mg/g DW) biosynthesis. In all conditions, the ratio of rebaudioside A/stevioside ranged from 0.75 to 1.12. The phenolic acids chlorogenic, neochlorogenic, isochlorogenic A, and rosmarinic were confirmed in the stevia extracts, as were the flavonoids isoquercetin, and quercitrin. The highest accumulations of chlorogenic and neochlorogenic acids and flavonoids were observed in shoot tissues derived from 5 µM mT, whereas 5 µM of BAP stimulated biosynthesis of chlorogenic, isochlorogenic A, and rosmarinic acids. This is the first report on the use of mT-cytokinin showing high potential in stevia cultures.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

Ptak,

Szewczyk,

Simlat

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…A large number of studies have been published where different plants have been produced in vitro to ensure propagation of rare genotypes and ensure their survival ( Zhou and Wu, 2006 ). Some examples include Psoralea coryfolia to produce daidzein and genistein ( Shinde et al., 2009 ), galanthamine production by Crinum malabaricum ( Priyadharshini et al., 2020 ; Chahal et al., 2022 ) or Eryngium alpinum to produce phenolic compounds ( Kikowska et al., 2020 ). However, in most cases, a protocol to obtain cell cultures for bioreactor production has not been developed, suggesting that it may be a good opportunity for future development.…”

Section: Cell Types Used In Bioreactorsmentioning

confidence: 99%

The advent of plant cells in bioreactors

Verdú-Navarro,

Moreno-Cid,

Weiss

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Ever since agriculture started, plants have been bred to obtain better yields, better fruits, or sustainable products under uncertain biotic and abiotic conditions. However, a new way to obtain products from plant cells emerged with the development of recombinant DNA technologies. This led to the possibility of producing exogenous molecules in plants. Furthermore, plant chemodiversity has been the main source of pharmacological molecules, opening a field of plant biotechnology directed to produce high quality plant metabolites. The need for different products by the pharma, cosmetics agriculture and food industry has pushed again to develop new procedures. These include cell production in bioreactors. While plant tissue and cell culture are an established technology, beginning over a hundred years ago, plant cell cultures have shown little impact in biotechnology projects, compared to bacterial, yeasts or animal cells. In this review we address the different types of bioreactors that are currently used for plant cell production and their usage for quality biomolecule production. We make an overview of Nicotiana tabacum, Nicotiana benthamiana, Oryza sativa, Daucus carota, Vitis vinifera and Physcomitrium patens as well-established models for plant cell culture, and some species used to obtain important metabolites, with an insight into the type of bioreactor and production protocols.

show abstract

“…High phenolic and antioxidant activity-containing medicinal plants and species such as Chanca Piedra ( Phyllanthus nirui L.), Yerba Mate ( Ilex paraguariensis St-Hil), Zarzaparrilla ( Smilax officinalis ), and Huacatay ( Tagetes minuta ) have the highest anti-hyperglycemia-relevant in vitro α-glucosidase inhibitory activities with no effect on α-amylase [ 69 ]. Nineteen phenolic compounds from different groups are used in wound treatment, and the compounds are tyrosol, curcumin, hydroxytyrosol, luteolin, rutin, chrysin, kaempferol, quercetin, icariin, epigallocatechin gallate, morin, silymarin, taxifolin, hesperidin, naringin, puerarin, isoliquiritin, genistein, and daidzein [ 70 , 71 , 72 , 73 ]. The most important identified phenolics in Phlomis angustissima and Phlomis fruticosa , medicinal plants from Turkey, by RP-HPLC-DAD were hesperidin, catechin, kaempferol, epicatechin, eupatorin, and epigallocatechin, and chlorogenic, syringic, vanillic, p -coumaric, ferulic, and benzoic acids [ 74 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Phenolic Compounds in Medicinal Plants—Natural Health Products for Human Health

2023

View full text Add to dashboard Cite

Phenolic compounds and flavonoids are potential substitutes for bioactive agents in pharmaceutical and medicinal sections to promote human health and prevent and cure different diseases. The most common flavonoids found in nature are anthocyanins, flavones, flavanones, flavonols, flavanonols, isoflavones, and other sub-classes. The impacts of plant flavonoids and other phenolics on human health promoting and diseases curing and preventing are antioxidant effects, antibacterial impacts, cardioprotective effects, anticancer impacts, immune system promoting, anti-inflammatory effects, and skin protective effects from UV radiation. This work aims to provide an overview of phenolic compounds and flavonoids as potential and important sources of pharmaceutical and medical application according to recently published studies, as well as some interesting directions for future research. The keyword searches for flavonoids, phenolics, isoflavones, tannins, coumarins, lignans, quinones, xanthones, curcuminoids, stilbenes, cucurmin, phenylethanoids, and secoiridoids medicinal plant were performed by using Web of Science, Scopus, Google scholar, and PubMed. Phenolic acids contain a carboxylic acid group in addition to the basic phenolic structure and are mainly divided into hydroxybenzoic and hydroxycinnamic acids. Hydroxybenzoic acids are based on a C6-C1 skeleton and are often found bound to small organic acids, glycosyl moieties, or cell structural components. Common hydroxybenzoic acids include gallic, syringic, protocatechuic, p-hydroxybenzoic, vanillic, gentistic, and salicylic acids. Hydroxycinnamic acids are based on a C6-C3 skeleton and are also often bound to other molecules such as quinic acid and glucose. The main hydroxycinnamic acids are caffeic, p-coumaric, ferulic, and sinapic acids.

show abstract

Meta-topolin-mediated regeneration and accumulation of phenolic acids in the critically endangered medicinal plant Crinum malabaricum (Amaryllidaceae): A potent source of galanthamine

Cited by 15 publications

References 45 publications

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

The advent of plant cells in bioreactors

Therapeutic Potential of Phenolic Compounds in Medicinal Plants—Natural Health Products for Human Health

Contact Info

Product

Resources

About