Metabacillus sediminilitoris sp. nov., a marine bacterium isolated from a tidal sediment

Mao, Haiyan; Wei, Yuli; Gao, Yuxin; Pei, Jiahao; Zhang, Yan; Fang, Jiasong

doi:10.1099/ijsem.0.004392

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…On the other hand, Metabacillus sediminilitoris obtained in this study was also characterized before as Gram-stain-positive, endospore-forming bacteria, grow at 16-47°C and alkaline pH 6.0-10.0 and tolerate NaCl up to 7% (w/v). The G+C content of its genomic DNA was 35.7mol% (36). Species of the genus Metabacillus are ubiquitous in natural environments and have been isolated from a wide variety of habitats, including soil, a hypersaline lake and a marine coastal region (37).…”

Section: Resultsmentioning

confidence: 99%

Molecular Biodiversity of Bacteria Isolated from Medicago sativa Rhizosphere in Haʼil District, Saudi Arabia

Sulieman¹,

Idris²,

Alshammari

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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Worldwide biodiversity is impacted strikingly by global environmental change, and thus its impact is reflected in all life aspects. Identifying microorganisms in environmental samples, particularly soil could be a valuable interest to study their effect on soil quality and plant growth. Through this study, we conducted a molecular characterization of bacteria found in the rhizosphere of Medicago sativa plants grown in Hai'l soil and we highlighted their main properties. The analysis of sequences revealed that the main bacterial isolates were Pseudarthrobacter, Metabacillus, Priestia, and Massilia species. According to the sequence analyses and the phylogeny tree results, some of the identified bacteria were classified at the species level: one of the pseudarthrobacter isolates was identified clearly as Pseudarthrobacter phenanthrenivorans; Metabacillus isolates grouped with Metabacillus sediminilitoris and the two Priestia isolates closely related to Priestia aryabhattai. We concluded that Hai'l soil is a niche of diverse bacteria with a high interest in soil environment and ecosystems. Further studies are required for further classification of all identified bacteria and to define their specific role in the environment.

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Section: Resultsmentioning

confidence: 99%

Molecular Biodiversity of Bacteria Isolated from Medicago sativa Rhizosphere in Haʼil District, Saudi Arabia

Sulieman¹,

Idris²,

Alshammari

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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“…The DNA G+C content of BG109 T was 35.71 mol%. *Data were taken from previous studies [21][22][23][24]. All other data were obtained from this study.…”

Section: Genome Featuresmentioning

confidence: 99%

Metabacillus bambusae sp. nov., isolated from bamboo grove soil

Jeong

Kim

2022

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization. BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium. BG109T showed growth at 10–40 °C (optimum, 37 °C), at pH 4–10 (optimum, 8), and in the presence of 0–7 % NaCl concentration (optimum, 0–1 %). The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso–diaminopimelic acid as the diagnostic diamino acid. The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and unidentified phospholipids and glycolipids were also present. The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0. The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus . BG109T shared highest 16S rRNA gene sequence similarities with ‘Metabacillus elymi’ KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction. The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus . Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus , for which the name Metabacillus bambusae sp. nov. is proposed. The type strain is BG109T (=KCTC 43190T=JCM 34515T).

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“…Test panels were used according to the manufacturer's instructions. Sensitivity to various antibiotics was tested on MA using different antibiotic discs (μg per disc): amoxicillin (10), streptomycin (10), cefoxitin (30), rifampicin (5), erythromycin (15), gentamicin (10), ampicillin (10), kanamycin (30), tetracycline (30), chloramphenicol (30), neomycin (30) and bacitracin (0.04 IU per disc).…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…At the time of writing (February 2022), the genus Metabacillus comprised 16 species with valid names (https://lpsn.dsmz.de/genus/metabacillus). Members of the genus Metabacillus have been isolated from a wide range of environments such as oysters [2], pebbles [3], faeces [4], blood [5], salt lake [6, 7], sediment [8–10] and wastewater [11]. Cells of the genus Metabacillus are Gram-stain-positive or Gram-stain-variable, aerobic, rod-shaped, motile or non-motile and grow at 4–45 °C.…”

Section: Full-textmentioning

confidence: 99%

Metabacillus kandeliae sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant Kandelia candel

Cai¹,

Huang²,

Liu

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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In this study, we isolated a novel Gram-stain-positive, aerobic, motile, rod-shaped bacterium, named GX 13764T, from the rhizosphere soil of a decayed mangrove plant Kandelia candel collected from Beihai, Guangxi, PR China, and characterized using a polyphasic taxonomic approach. The strain exhibited yellow-orange, round, convex, shiny, smooth, opaque and 2–3 mm diameter colonies on marine agar 2216 media after 3 days of incubation at 30 °C and was capable of growth at 4–45 °C (optimum, 30 °C), pH 5.0–9.0 (optimum, pH 7.0) and 0–4 % NaCl (w/v; optimum, 2 %). The strain was positive for catalase and negative for the oxidase. The main cellular fatty acid was anteiso-C15:0, iso-C15:0, iso-C16:0 and iso-C14:0. The cell-wall peptidoglycan comprised meso-diaminopimelic acid and the main menaquinone was MK-7. The polar lipids included one diphosphatidylglycerol, one phosphatidylglycerol, two glycolipids, two unidentified phospholipids and three unidentified lipids. Based on 16S rRNA gene analysis, GX 13764T presented the highest sequence similarity to Metabacillus mangrovi KCTC 33872T (97.04 %). The DNA G+C content of the type strain was 44.2 mol%. The average nucleotide identity values between GX 13764T and M. mangrovi KCTC 33872T, Metabacillus idriensis DSM 19097T and Metabacillus indicus LMG 22858T were 69.39, 68.87 and 68.95 %, respectively, with digital DNA–DNA hybridization values of 19.9, 19.5 and 19.5 %, respectively. Based on the polyphasic data, strain GX 13764T should be nominated as a novel species of the genus Metabacillus , for which the name Metabacillus kandeliae sp. nov. is proposed. The type strain is GX 13764T (=MCCC 1K06654T=KCTC 43366T).

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Metabacillus sediminilitoris sp. nov., a marine bacterium isolated from a tidal sediment

Cited by 11 publications

References 23 publications

Molecular Biodiversity of Bacteria Isolated from Medicago sativa Rhizosphere in Haʼil District, Saudi Arabia

Molecular Biodiversity of Bacteria Isolated from Medicago sativa Rhizosphere in Haʼil District, Saudi Arabia

Metabacillus bambusae sp. nov., isolated from bamboo grove soil

Metabacillus kandeliae sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant Kandelia candel

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