Metabiotics and their Health Benefits

Shaikh, Aijaz; Sreeja, V.

doi:10.5958/2321-712x.2017.00002.3

Cited by 16 publications

(4 citation statements)

References 108 publications

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“…Bioactive peptides with various functionalities have been identified in several dairy-products such as cheese, fermented milk, milk protein hydrolysates and various meat products [73]. Their origin and various functionalities are summarized in Table 1 [44] biotin, nicotinic acid, cobalamine, riboflavin, thiamine, pyridoxine and pantothenic acid in humans [81] [82]. Therefore, the magnificent property of probiotics for the production of vitamins has been envisaged as sustainable alternative for the bio-fortification as compared with chemically synthesized pseudovitamins.…”

Section: Bioactive Peptidesmentioning

confidence: 99%

“…A variety of other anti-microbial actions have been documented for the various probiotics species to combat infections like urogenital tract infections [36] [37], Clostridium [38], L.monocytogenes [39] [40], candidiasis and mastitis infection [41], gastrointestinal and pneumococcal infections [42] [43]. Tough the food preservation has been considered the major application of the bacteriocins but growing knowledge regarding their structural and functional diversity leads to various attempts for elucidating either the more comprehensive functions or improves the functionality of these molecules nowadays [44].…”

Section: Polyaminesmentioning

confidence: 99%

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Metabiotics: The Functional Metabolic Signatures of Probiotics: Current State-of-Art and Future Research Priorities—Metabiotics: Probiotics Effector Molecules

Singh¹,

Vishwakarma²,

Singhal³

2018

ABB

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The intricate "orchestered molecular conversation" between the host and gut microbiome is one of the most dynamic research areas in recent years. The rhythmic chemical cross talk in the form of bioactive metabolites and signalling molecules synthesized by gut microbiome plays a significant role for the modulation of human health in diversified ways. They are recognized as low molecular weight (LMW) molecules having versatile chemical attributes. They possess magnificent capability of interacting with surrounding environment and controlling the genes for various genetic, biochemical and physiological functions for maintaining the homeostasis that is now-a-days termed as "small molecules microbes originated (SMOM) homeostasis" in the host. These metabolic signatures have close structural and functional resemblance with small molecules synthesized by host eukaryotic cells and dietary components. Therefore, they may be considered as universalized metabolites contributing to the remarkable phenomenon of epigenetic regulation, cell to cell communication and stability of genome manifesting the overall growth and development of the host and known as "metabiotics". The wide panorama of utilization of probiotics is continuously expanding and conferring the major health benefits through metabiotic components are gaining tremendous momentum therefore recognized as "hidden soldiers" of the body. Therefore firstly, we outline the need and types of metabiotic molecules and depicting their role in human health. Then, we summarize their preventive and therapeutic avenues in various diseases and finally, we propose the current technological interventions, bottlenecks and future perspectives in this field that are implied for accelerating their comprehensive understanding and utilization at industrial scale.

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Section: Bioactive Peptidesmentioning

confidence: 99%

Section: Polyaminesmentioning

confidence: 99%

Metabiotics: The Functional Metabolic Signatures of Probiotics: Current State-of-Art and Future Research Priorities—Metabiotics: Probiotics Effector Molecules

Singh¹,

Vishwakarma²,

Singhal³

2018

ABB

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“…Нові речовини та сполуки штучного та природного походження, а також створені на їх основі лікувально-профілактичні засоби потребують як експериментального доклінічного випробування щодо певних біологічних властивостей, так і проведення токсикологічних досліджень (Stefanov, 2001;Habriev, 2005). Розроблення препаратів нового покоління на основі бактеріальних похіднихструктурних компонентів і продуктів життєдіяльності пробіотичних мікроорганізмівметабіотиків, які мають низку переваг порівняно з клітинними препаратами, наразі перспективне й актуальне (Shenderov, 2013;Shaikh & Sreeja, 2017;Shenderov & Gabrichevsky, 2017). Метаболіти пробіотичних мікроорганізмів мають стимулювальний вплив на нормальну мікрофлору (Rivière et al, 2016), виявляють протимікробну (Lew & Liong, 2013;Presti et al, 2015;Sharma et al, 2017), імуномодуляторну (Jensen et al, 2010;Bauer et al, 2013;Kang & Connolly, 2016), протизапальну (Griet et al, 2014;Richards et al, 2016;Castiblanco et al, 2017), противірусну (Kim et al, 2014;Ang et al, 2016;Al Kassaa, 2017), протипухлинну (Sharma & Shukla, 2016) та інші види активності.…”

Section: вступunclassified

Cellular metabolic activity as a marker of cytotoxicity and immunotropicity of probiotics’ derivatives

Knysh

Isayenko

Falko

et al. 2018

Regul. Mech. Biosyst.

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Structural components of cells and metabolites of probiotics with biologically active potential, along with the study of effectiveness, require a series of tests to ensure their safety. The study aims to test the cytotoxicity and potential of structural and metabolic derivatives of Bifidobacterium bifidum and Lactobacillus reuteri to affect the immunocompetent cells using in vitro tests that characterize the metabolic activity of test-cells. Structural components of probiotic bacteria were obtained by the physical method of disintegration – cyclic freezing-thawing. Metabolic derivatives were obtained by cultivation of producers – bifidobacteria and lactobacilli in their own disintegrates. Cultures of mouse embryonic fibroblasts and splenocytes were used as the test cells. MTT and Alamar Blue® were used as redox indicators. According to the MTT test, filtrates that contain structural and metabolic derivates at a concentration of 5% and 10% in the incubation medium did not cause significant changes in the metabolic activity of the embryonic mouse fibroblasts. An increase of up to 20% of content in the incubation medium of filtrates of lactobacilli disintegrates led to a reduction of metabolic activity of test cells by 52.7 ± 6.2%, of filtrates of bifidobacteria disintegrates – by 26.5 ± 6.5%, of filtrates of lactobacterium culture – by 15.7 ± 6.9%, of filtrates of bifidobacterium cultures - by 40.4 ± 6.8%. According to the Alamar Blue® test, filtrates that contained only structural derivatives of lactobacilli and bifidobacteria at concentrations of 5% and 10%, as well as filtrates that contained a complex of structural and metabolic derivatives at a concentration of 5%, did not cause significant changes in the reducing ability of mouse splenocytes. At concentrations of 10%, filtrate containing a complex of structural and metabolic derivatives of lactobacilli, caused the inhibition of metabolic activity of splenocytes by 14.6 ± 3.5%, and bifidobacteria – by 10.0 ± 2.8%. With the contents of the incubation medium at 20% concentration, the filtrate of the disintegrates of lactobacilli decreased the metabolic activity of splenocytes by 12.2 ± 3.0%, and the filtrate of lactobacillus cultures that were grown on their own disintegrates – by 43.2 ± 3.3%. Increasing the content of the disintegrate filtrate and the bifidobacteria culture that were grown on their own disintegrates in the culture medium by up to 20% led to a decrease the metabolic activity of splenocytes by 38.0 ± 2.0%. Thus, the research has shown: the orientation of changes in cellular metabolism under the influence of the studied biologically active derivatives is similar in all model systems, and their intensity depends on the type of test cells, regenerative substrates and concentration of the agent of influence in model systems. The obtained results stimulate further exploration of the immunotropicity of the investigated derivatives of probiotic bacteria and can be used for development of new immunobiological preparations.

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“…До переваг метабіотиків, крім більшого терапевтичного потенціалу, відносять високу біодоступність, відсутність конфлікту з власною мікробіотою, відсутність латентного періоду, безпеку, вищі споживчі властивості та терміни придатності [2,3].…”

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The study on the development of the composition of chewable lozenges with probiotic properties. 1. Substantiation of the active substance

Dvinskykh,

Khokhlenkova,

Kaliuzhnaia

et al. 2024

News of pharm.

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In recent times, a new understanding of the concept of probiotics has emerged – the use of their metabolites instead of living cells of microorganisms. They can both inhibit the development of pathogenic bacteria and effectively stimulate the reproduction of indigenous microflora. It is possible that metabiotics will be more effective than traditional live probiotics. Taking into account the prospects of new-generation probiotic drugs and the rapid growth of interest of pharmaceutical market entities in such a new dosage form as lozenges, we have proposed to create chewable lozenges containing Lactobacillus exometabolites, which pharmacological action is aimed at preventing and treating an imbalance of the oral cavity microflora.Aim. To theoretically and experimentally substantiate the choice of the exometabolite complex of lactobacilli as the active substance of a medicinal product with probiotic properties in the form of chewable lozenges.Materials and methods. The exometabolite complex of the Lactobacillus plantarum UCM-2693 strain was used as the study object. Acidity, the lactic acid content, the optical density of a microbial suspension, as well as the quantitative count of lactobacilli were determined in accordance with the generally accepted methods specified in the State Pharmacopoeia of Ukraine (SPhU).Results and discussion. The bacteriotropic effect of the exometabolite complex of L. plantarum UCM-2693 was detected on lactobacilli of the probiotic drug “Lactobacterin-Biopharma” when cultured on a medium with exometabolites compared to the control sample. This technique has a stimulating effect on the acid formation, proliferation of probiotic microorganisms, changes the characteristics of their growth phases, namely reduces the lag phase, accelerates the exponential phase, which can accelerate the onset of the therapeutic effect, and is the proof of the feasibility of using this active substance in the composition of chewable lozenges with probiotic properties.Conclusions. The choice of the exometabolite complex of lactobacilli as the active substance of a new drug from the group of metabiotics in the form of chewable lozenges has been substantiated. The exometabolite complex of lactobacilli has been obtained, and its bacteriotropic effect has been studied.

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Metabiotics and their Health Benefits

Cited by 16 publications

References 108 publications

Metabiotics: The Functional Metabolic Signatures of Probiotics: Current State-of-Art and Future Research Priorities—Metabiotics: Probiotics Effector Molecules

Metabiotics: The Functional Metabolic Signatures of Probiotics: Current State-of-Art and Future Research Priorities—Metabiotics: Probiotics Effector Molecules

Cellular metabolic activity as a marker of cytotoxicity and immunotropicity of probiotics’ derivatives

The study on the development of the composition of chewable lozenges with probiotic properties. 1. Substantiation of the active substance

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