Metabolic activity of plant mitochondria in hypertonic sucrose solutions

Шугаев, А. Г.; Генерозова, И. П.; Shugaeva, N. A.; Vyskrebentseva, Ė. I.

doi:10.1134/s1021443708030084

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…The attention is drawn to fine regulation of respiratory metabolism of pea seedlings in response to growth conditions, which was manifested in the rapid response of plants to very small (only by several grades) temperature lowering. The results of this work also confirmed a possibility to use isolated mitochondria as indicators of osmotic stress, which corresponds to conclusions from our previous investigations [5,6].…”

Section: Discussionsupporting

confidence: 92%

“…5a, curves 2 and 4), in treatment S 3 a drop in oxidation of malate and glutamate in mitochondria in state 3 dur ing dehydration was much stronger than in treatment S 2 . Earlier, we have demonstrated that, at the increase in the concentration of osmotically active compounds in medium, the value of V 3 started to decline with a less rate after a definite concentration (0.75 M sucrose) [5]. In this work, it was shown that, in 2 day old seed lings ( ), the content of water in epicotyl tissues was similar to that in 3 day old seedlings after two days in the presence of osmoticum, i.e., in epicotyls it was 91.5% (Fig.…”

Section: Discussionmentioning

confidence: 95%

“…For successful survival, plants use diverse defense mechanisms, modifying metabolic reactions, and this permits them to adapt to variable effects of habitats. Unfavorable environmental factors are known to inhibit to some extent respiration in mitochondria, reducing its efficiency due to partial suppression of oxidative phosphorylation [1][2][3][4][5][6], and to retard seed ling growth [1,2]. We have demonstrated earlier that, in spite of high sensitivity of mitochondria in etiolated epicotyls of 3 day old pea seedlings to osmotic stress and water loss, mitochondria were capable of main taining the respiratory control (RC) at the high osmotic pressure of the medium [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Respiration metabolism in mitochondria of pea seedlings of different age under water deficit and rewatering

Генерозова

Шугаев

2012

Russ J Plant Physiol

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The effects of an osmoticum (mannitol) on growth, water content, respiration, and proline con tent in epicotyls of etiolated two day old (S 2 ) and three day old (S 3 ) pea (Pisum sativum L., cv. Flora 2) seedlings were studied. Water deficit was produced by placing plant roots in 0.6 M mannitol for 2-4 days. Control S 3 epicotyls contained more water than S 2 epicotyls; they were also characterized by more intense mitochondrial activity (in state 3, V 3 ) and by the higher value of the respiratory control (RC) due to a fourfold decrease of proportion of weakly phosphorylating cyanide resistant respiration (CRR). After 2 day long treatment with the osmoticum, S 2 epicotyls lost more water than S 3 ; water deficit in them attained 40% (28% in S 3 ). In S 3 treatment, epicotyls continued to grow at the almost unchanged rate, and the content of proline in them changed insignificantly, whereas in S 2 seedlings epicotyl growth stopped, and proline accumulated in them. In the presence of the osmoticum, V 3 and RC reduced, but in S 3 treatment they retained higher than in treatment S 2 . In mitochondria of S 3 subjected to dehydration, the proportion of the cytochromic pathway of oxidation (V cyt ) remained high, but the absolute values of the respiration rate reduced, whereas in S 2 both these indices reduced and the proportion of CRR increased. After rewatering, indices of mitochondrial activ ity (the rate of substrate oxidation, RC value) were restored, but in S 3 they were higher. The ratio of V cyt to CRR in S 3 shifted toward CRR, whereas in S 2 , in contrast, it was shifted toward V cyt . When temperature dur ing seedling growth and experiment performing was declined from 22°C (in control) to 18°C, growth of S 3 epicotyls was retarded to the rate characteristic of S 2 at 22°C. Under the effect of osmoticum, they, like S 2 , acquired a capacity to accumulate proline. Nevertheless, mitochondrial activity in such S 3 turned out to be higher than in S 2 grown at 22°C. The results obtained than, as distinct from traditional notes, 3 day old pea seedlings manifested the higher tolerance to water deficit than 2 day old seedlings.Abbreviations: AO-alternative oxidase; RC-respiratory con trol; S 2 and S 3 -2 and 3 day old seedlings, respectively, sub jected to osmoticum action and subsequent rewatering; SHAMsalicylhydroxamic acid; TMPD-N,N,N',N' tetramethylphe nylendiamine; V 3 -the respiration rate in mitochondria in state 3; V 4 -the respiration rate in mitochondria in state 4; V cytactivity of cytochromic pathway for oxidation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Respiration metabolism in mitochondria of pea seedlings of different age under water deficit and rewatering

Генерозова

Шугаев

2012

Russ J Plant Physiol

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“…Guanidine at low concentrations did not increase the rate of respiration and mitochondria phosphorylation ability unlike sucrose [1,4,21], but at high concentrations inhibited the functional activity of mitochondria. Our data are in agreement with literature data that guanidines cause an inhibition of mitochondrial respiration [7,9,11] and guanidinies do not inhibit mitochondrial swelling mediated by energy derived from either substrate oxidation or by externally added ATP [22].…”

Section: Discussionmentioning

confidence: 75%

On the Mechano-Chemiosmotic Mechanism of Action of Guanidinies on Functional Activity of Mitochondria and Aging

Kasumov¹

2014

Organic Chem Curr Res

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“…As noted above, the results obtained indicate the absence of any correlation between the membrane lipid peroxidation level and the extent of inhibition of respiratory activity immediately after treatments. This finding may imply that osmotic stress but not oxidative stress is capable of modifying the properties of the inner mitochondrial membrane, and it is the main reason for electron transfer inhibition of the ETC (Mathai et al 1993, Shugaev et al 2008, Generosova et al 2009.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial respiration after combined action of dehydration and low temperature in pea seedlings

Генерозова¹,

Буцанец²,

Шугаев³

2019

Biologia plant.

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Metabolic activity of plant mitochondria in hypertonic sucrose solutions

Cited by 5 publications

References 21 publications

Respiration metabolism in mitochondria of pea seedlings of different age under water deficit and rewatering

Respiration metabolism in mitochondria of pea seedlings of different age under water deficit and rewatering

On the Mechano-Chemiosmotic Mechanism of Action of Guanidinies on Functional Activity of Mitochondria and Aging

Mitochondrial respiration after combined action of dehydration and low temperature in pea seedlings

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