Metabolic characteristics of muscles in the spiny lobster, Jasus edwardsii, and responses to emersion during simulated live transport

Speed, Steven R; Baldwin, John J.; Wong, Ron; Wells, R. M. G.

doi:10.1016/s1096-4959(00)00340-7

Cited by 78 publications

(43 citation statements)

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“…These slight differences are mostly due to varied concentrations of free arginine present in the muscle. Arginine phosphate in blue crab leg muscle (29.6mol g -1 ; Table1) was similar to that found in blue crab levator muscle (29.9molg -1 ) (Hardy et al, 2006) and in spiny lobster leg muscle (31.35mol g -1 ) (Speed et al, 2001), but higher than concentrations found in highly aerobic ghost crabs (21.5molg -1 ) (Full and Weinstein, 1992). ATP content (5.7mol g -1 ; Table 2), indicating muscle energetic condition, was comparable to concentrations in red crab leg muscle (4.76 mol g -1 ) (Morris and Adamczewska, 2002) and in spiny lobster leg muscle (5.14molg -1 ) (Speed et al, 2001) but lower than ATP content measured in crayfish tail muscle (7.43molg -1 ) (Head and Baldwin, 1986) and in blue crab levator muscle (7.3molg -1 ) (Hardy et al, 2006).…”

Section: Discussionsupporting

confidence: 80%

“…In the walking leg muscle, arginine phosphate was found in high concentrations (25-30molg -1 ) along with comparatively lower concentrations of arginine (15-20molg -1 ; Table1). This corresponds to a resting arginine charge of 0.66, which is comparable to 0.69 found in blue crab levator muscle (Hardy et al, 2006) and 0.59 in crayfish leg muscle (Speed et al, 2001) but lower than 0.8 in red crab leg muscle (Morris and Adamczewska, 2002). These slight differences are mostly due to varied concentrations of free arginine present in the muscle.…”

Section: Discussionsupporting

confidence: 48%

“…ATP content (5.7mol g -1 ; Table 2), indicating muscle energetic condition, was comparable to concentrations in red crab leg muscle (4.76 mol g -1 ) (Morris and Adamczewska, 2002) and in spiny lobster leg muscle (5.14molg -1 ) (Speed et al, 2001) but lower than ATP content measured in crayfish tail muscle (7.43molg -1 ) (Head and Baldwin, 1986) and in blue crab levator muscle (7.3molg -1 ) (Hardy et al, 2006). The resting adenylate energy charge in blue crab leg muscle (0.89) was high (Table2) but very comparable to the energy charge found in other crustacean muscles (England and Baldwin, 1983;Speed et al, 2001;Morris and Adamczewska, 2002).…”

Section: Discussionmentioning

confidence: 58%

“…High concentrations of arginine phosphate and ATP are generally associated with muscle fibers that predominantly use anaerobic energy production (England and Baldwin, 1983;Head and Baldwin, 1986;Speed et al, 2001). Although the muscle tissue analyzed here most likely contained a combination of aerobic and anaerobic muscle fibers and no attempt was made to distinguish between the two fiber L. K. Thibodeaux, K. G. Burnett and L. E. Burnett types, phosphagen energy stores are clearly important during walking as shown by a pronounced 57% depletion in arginine phosphate associated with a 55% increase in free arginine, causing a dramatic drop in arginine charge from 0.66 to 0.35 (Table1).…”

Section: Discussionmentioning

confidence: 99%

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Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

Thibodeaux

Burnett

2009

Journal of Experimental Biology

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SUMMARYCallinectes sapidus (Rathbun), the Atlantic blue crab, commonly harbors low to moderate amounts of bacteria in hemolymph and other tissues. These bacteria are typically dominated by Vibrio spp., which are known to cause mortality in the blue crab. The dose-dependent lethality of an isolate of Vibrio campbellii was determined in crabs; the mean 48h LD 50 (half-maximal lethal dose) was 6.2ϫ10 5 colony forming unitsg -1 crab. Injection of a sublethal dose of V. campbellii into the hemolymph of the crab resulted in a rapid and large depression (30-42%) of metabolic rate, which persisted for 24h. Because gills are an organ of immune function as well as respiration, we were interested in how bacteria injected into the crab would affect the energetic costs associated with walking. Overall metabolism (aerobic and anaerobic) more than doubled in crabs walking for 30min at 8mmin -1 . The metabolic depression resulting from bacterial injection persisted throughout the exercise period and patterns of phosphagen and adenylate consumption within walking leg muscle were not affected by treatment. The ability of crabs to supply required energy for walking is largely unaffected by exposure to Vibrio; however, Vibrio-injected crabs are less aerobic while doing so. This depressed metabolic condition in response to bacteria, present during moderate activity, could be a passive result of mounting an immune response or may indicate an actively regulated metabolic depression. A compromised metabolism can affect the performance of daily activities, such as feeding and predator avoidance or affect the ability to cope with environmental stressors, such as hypoxia.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

Thibodeaux

Burnett

2009

Journal of Experimental Biology

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“…Morris & Adamczewska (2002) showed that brief exercise in the terrestrial air-breathing crab Gecarcoidea natalis (Pocock, 1888) depletes arginine phosphate stores, consumes glycogen and results in the accumulation of L-lactate within the walking leg muscles. The tail-flip escape swimming of the spiny lobster Jasus edwardsii (Hutton, 1875) induces extensive anaerobic glycolysis in the abdominal muscles (Speed et al 2001). In Nephrops norvegicus it was shown that not only anoxia (through aerial exposure) but also the hypoxic conditions (10Á30% O 2 saturation) in the bottom water of certain fishing grounds cause significant glycogen depletion, thus indicating anaerobic fermentation (Baden et al 1994).…”

Section: Introductionmentioning

confidence: 99%

The influence of defined ante-mortem stressors on the early post-mortem biochemical processes in the abdominal muscle of the Norway lobster,Nephrops norvegicus(Linnaeus, 1758)

Gornik

Albalat

Atkinson³

et al. 2010

Marine Biology Research

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The effects of four different ante-mortem stressors (exercise, emersion, starvation and a patent infection with the parasite Hematodinium sp.) on post-mortem processes have been investigated in the abdominal muscle of Norway lobster Nephrops norvegicus by measuring changes in the pH, the levels of glycogen, L-lactate, arginine phosphate, ATP, ADP, AMP, IMP, HxR, Hx and the adenylate energy charge (AEC) over a time course of 24 h with samples being taken at 0, 3, 6, 12 and 24 h. The acute stresses of intense exercise and 2 h emersion resulted in a premature onset of anaerobic glycolysis, leading both to an enhanced glycogen depletion rate and an early accumulation of L-lactate. The chronic stressors, starvation and parasite infection, resulted in a complete ante-mortem depletion of muscle glycogen and consequently the failure of post-mortem glycolytic fermentation. Post-mortem pH and ATP inter-conversion were significantly altered in chronically stressed animals. Ante-mortem, a rapid, almost complete depletion of arginine phosphate was observed in all stress groups. The AEC was altered significantly by all stresses, indicating a strong energy demand. The findings suggest that ante-mortem stressors strongly influence the post-mortem biochemical processes. The laboratory-based results are compared to 'field' data and effects on post-harvest product quality are discussed.

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Impacts of sub‐optimal water quality on the haemolymph biochemistry, immune status and condition of post‐harvest Southern Rock Lobsters (Jasus edwardsii)

Day

Fitzgibbon

Gardner

2022

Aquaculture Fish & Fisheries

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Palinurid rock (or spiny) lobsters constitute valuable seafood fisheries around the world. Post‐harvest mortality can have a substantial economic impact on the value of these fisheries and damage the brand identity of an otherwise fungible commodity. In this study, sudden, large‐scale mortalities in the Australian Rock Lobster industry were investigated through comprehensive haemolymph biochemistry panels, measurements of immune status and nutritional condition. A cohort of lobsters (n = 25) was sampled over a 4‐week period at a commercial processing facility, with the final sample occurring during a mortality event. A comparison of 22 biochemical parameters, including electrolytes, minerals, ions, metabolites and enzymes, showed a notable disruption in osmoregulation, protein content and enzymes during weeks 3 and 4 of the study. Haemocyte counts showed a decline in circulating haemocytes by the time of the mortality event along with a shift in the proportion of hyalinocytes to granulocytes favouring the former. The condition index was variable over the sampling schedule, possibly linked to observed incidences of cannibalism, with no evidence of an overall low condition that would explain mortalities. The lobsters sampled in this study did not show any consistent or progressive changes in biochemistry that may indicate a pathogen or disease as a causative agent. Rather, analysis of the water quality within the facility suggested that mortality was due to sub‐optimal water quality characterized by a combination of two factors, a substantial decrease in the pH and potential nitrogen toxicity, that caused a physiological response comprised of an ion imbalance, altered enzyme activity and, ultimately, mortality. These results offer a comprehensive characterization of the impact of unexplained morbidity on the biochemistry and physiology of lobsters, illustrate the harm of sub‐optimal aquaculture practices and highlight the need for a better understanding of aquaculture practices and their effects on economically important seafood industries.

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Metabolic characteristics of muscles in the spiny lobster, Jasus edwardsii, and responses to emersion during simulated live transport

Cited by 78 publications

References 22 publications

Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

Energy metabolism and metabolic depression during exercise inCallinectes sapidus, the Atlantic blue crab: effects of the bacterial pathogenVibrio campbellii

The influence of defined ante-mortem stressors on the early post-mortem biochemical processes in the abdominal muscle of the Norway lobster,Nephrops norvegicus(Linnaeus, 1758)

Impacts of sub‐optimal water quality on the haemolymph biochemistry, immune status and condition of post‐harvest Southern Rock Lobsters (Jasus edwardsii)

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