Metabolic characterization of diabetic retinopathy: An 1H-NMR-based metabolomic approach using human aqueous humor

Jin, Huiyi; Zhu, Bijun; Liu, Xia; Jin, Jing; Zou, Haidong

doi:10.1016/j.jpba.2019.06.013

Cited by 60 publications

(46 citation statements)

References 27 publications

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“…Metabolomics is a powerful method for studying pathophysiological processes. Metabolomics analysis of human samples can help clarify the mechanisms of AP and identify potential therapeutic targets [ 24 ]. The results of the OPLS-DA score plots revealed clear group separations among the blank control group, AP model group, and AP+rhein+honokiol group in our study.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Rhein and Honokiol on Metabolic Profiles in a Mouse Model of Acute Pancreatitis

Huang

Liu

et al. 2020

Med Sci Monit

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Section: Discussionmentioning

confidence: 99%

The Effects of Rhein and Honokiol on Metabolic Profiles in a Mouse Model of Acute Pancreatitis

Huang

Liu

et al. 2020

Med Sci Monit

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“…Of these, the levels of lactate and succinate were dramatically decreased, while asparagine, glutamine, histidine, and threonine were increased in patients with DR compared to diabetes patients without cataract. Data analysis revealed that lactate, succinate, 2-hydroxybutyrate, asparagine, dimethylamine, histidine, threonine, and glutamine were the most altered metabolites that potentially play roles in the development and progression of DR. Metabolic pathway analysis showed that over 11 pathways were influenced including alanine, aspartate and glutamate metabolism, aminoacyl-tRNA biosynthesis, propanoate, and nitrogen metabolism 82 . The detected alterations in amino acid metabolism are possibly related to mitochondrial dysfunction, oxidative stress and endothelial damage in patients with DR.…”

Section: Diabetic Retinopathymentioning

confidence: 99%

Metabolomic analysis in ophthalmology

Nazifova-Tasinova

Radeva

Galunska

et al. 2020

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Modern science takes into account phenotype complexity and establishes approaches to track changes on every possible level. Many "omics" studies have been developed over the last decade. Metabolomic analysis enables dynamic measurement of the metabolic response of a living system to a variety of stimuli or genetic modifications. Important targets of metabolomics is biomarker development and translation to the clinic for personalized diagnosis and a greater understanding of disease pathogenesis. The current review highlights the major aspects of metabolomic analysis and its applications for the identification of relevant predictive, diagnostic and prognostic biomarkers for some ocular diseases including dry eye, keratoconus, retinal diseases, macular degeneration, and glaucoma. To date, possible biomarker candidates for dry eye disease are lipid metabolites and androgens, for keratoconus cytokeratins, urea, citrate cycle, and oxidative stress metabolites. Palmitoylcarnitine, sphingolipids, vitamin D related metabolites, and steroid precursors may be used for distinguishing glaucoma patients from healthy controls. Dysregulation of amino acid and carnitine metabolism is critical in the development and progression of diabetic retinopathy. Further work is needed to discover and validate metabolic biomarkers as a powerful tool for understanding the molecular mechanisms of ocular diseases, to provide knowledge on their etiology and pathophysiology and opportunities for personalized clinical intervention at an early stage.

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“…NMR-based metabolomics can be used to identify metabolic reactions and potential biomarkers, it can analyse hundreds or even thousands of samples in a relatively short period of time with the help of an auto sample [13]. Meanwhile, metabolomics is a powerful approach for studying pathophysiological processes, and metabolomic analysis of human samples may shed light on the mechanisms of gastric mucosal and help identify potential therapeutic targets [14]. It can analysis changes of metabolic profiles caused by disease and other stimuli from a holistic view, which coincides exactly with the TCM theory [15].…”

Section: Introductionmentioning

confidence: 99%

Dynamic observation and analysis of metabolic response to moxibustion stimulation on ethanol-induced gastric mucosal lesions (GML) rats

et al. 2019

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Background Gastric mucosal lesion (GML) is the initiating pathological process in many refractory gastric diseases. And moxibustion is an increasingly popular alternative therapy that prevents and treats diseases. However, there are few published reports about developing pathology of GML and therapeutic mechanism of moxibustion treatment on GML. In this study, we investigated pathology of GML and therapeutic mechanism of moxibustion treatment on GML. Methods The male Sprague-Dawley (SD) rats were induced by intragastric administration of 75% ethanol after fasting for 24 h and treated by moxibustion at Zusanli (ST36) and Liangmen (ST21) for 1 day, 4 days or 7 days. Then we applied 1H NMR-based metabolomics to dynamic analysis of metabolic profiles in biological samples (stomach, cerebral cortex and medulla). And the conventional histopathological examinations as well as metabolic pathways assays were also performed. Results Moxibustion intervention showed a beneficial effect on GML by modulating comprehensive metabolic alterations caused by GML, including energy metabolism, membrane metabolism, cellular active and neurotransmitters function. Conclusions Moxibustion can effectively treat gastric mucosal damage and effectively regulate the concentration of some related differential metabolites to maintain the stability of the metabolic pathway.

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Metabolic characterization of diabetic retinopathy: An 1H-NMR-based metabolomic approach using human aqueous humor

Cited by 60 publications

References 27 publications

The Effects of Rhein and Honokiol on Metabolic Profiles in a Mouse Model of Acute Pancreatitis

The Effects of Rhein and Honokiol on Metabolic Profiles in a Mouse Model of Acute Pancreatitis

Metabolomic analysis in ophthalmology

Dynamic observation and analysis of metabolic response to moxibustion stimulation on ethanol-induced gastric mucosal lesions (GML) rats

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