Metabolic compensation in Arabidopsis thaliana catalase-deficient mutants

Buzduga, I. M.; Волков, Р. А.; Панчук, І. І.

doi:10.3103/s0095452718010036

Cited by 11 publications

(7 citation statements)

References 27 publications

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“…Activity of catalase (CAT) was evaluated in head and abdomen by measuring the decrease in hydrogen peroxide content in the samples, applying the previously described method (Korolyuk et al, 1988;Buzduga et al, 2018). 100-120 mg of tissues ground in liquid nitrogen were mixed with six volumes of extraction buffer (100 mM potassium phosphate, pH 7.4, 1 mM PMSF, and 1 mM EDTA), additionally homogenized on ice, and centrifuged for 20 min at 14,000 g, 4°C.…”

Section: Biochemical Measurementsmentioning

confidence: 99%

Increased survival of honey bees consuming pollen and beebread is associated with elevated biomarkers of oxidative stress

Yazlovytska

Karavan²,

Domaciuk³

et al. 2023

Front. Ecol. Evol.

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IntroductionSignificant losses of honey bee colonies have been observed worldwide in recent decades. Inadequate nutrition is considered to be one of the factors that can reduce honey bee resistance to abiotic and biotic environmental stresses. Accordingly, we assessed the impact of food composition on worker bee survival.MethodsBees in cages were fed six different diets, and then their survival, levels of thiobarbituric acid reactive substances and protein carbonyl groups, catalase and lysozyme activities were evaluated.Results and DiscussionAfter 17 days of feeding, the lowest mortality was observed in the group of bees that received sucrose solution with the addition of willow pollen or artificial rapeseed beebread or artificial willow beebread (diets 4–6). The highest mortality was found in bees that consumed only sucrose solution (diet 1) or the sucrose solution supplemented with a mixture of amino acids (diet 2), which can be explained by the lack of vitamins and microelements in these diets. In the group of bees that received the sucrose solution with rapeseed pollen (diet 3), mortality was intermediate. To check whether the decrease in insect survival could be related to oxidative damage, we evaluated biomarkers of oxidative stress. Consumption of pollen (diets 3 and 5) and artificial beebread (diets 4 and 6) enhances protein carbonylation in worker bees. Feeding bees artificial beebread also resulted in increase in lipid peroxidation and catalase activity, which is probably due to the presence of hydrogen peroxide in the honey contained in beebread. Remarkably, the increase in biomarkers of oxidative stress was not accompanied by adverse but positive effects on insect survival. A lack of amino acids and proteins in the diet 1 did not cause oxidative stress, but led to an increase in lysozyme activity in hemolymph, a biomarker of immune system status. In conclusion, we believe that the increase in oxidative stress biomarkers we found do not indicate oxidative damage, but rather reflect the changes in redox balance due to consumption of certain dietary options.

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Section: Biochemical Measurementsmentioning

confidence: 99%

Increased survival of honey bees consuming pollen and beebread is associated with elevated biomarkers of oxidative stress

Yazlovytska

Karavan²,

Domaciuk³

et al. 2023

Front. Ecol. Evol.

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“…To measure the activity of antioxidant enzymes, the plant material was homogenized in liquid nitrogen and the proteins were extracted using appropriate buffers [42,57]. Extraction buffer containing 0.1 M tris-HCl (pH 6.8), 20% glycerol, 30 mM dithiothreitol and 0.1% insoluble polyvinylpolypyrrolidone was used to determine the activity of CAT.…”

Section: Enzymes Activity Measurementmentioning

confidence: 99%

“…CAT activity was evaluated as described previously [57,59] by measuring the decrease in H 2 O 2 content in the sample. To stop the reaction, a solution of ammonium molybdate was added to the reaction mixture.…”

Section: Enzymes Activity Measurementmentioning

confidence: 99%

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Rapid Accumulation of Cadmium and Antioxidative Response in Tobacco Leaves

Buzduga¹,

Саламон²,

Волков³

et al. 2022

TOASJ

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Background: Cadmium (Cd) is one of the most dangerous environmental pollutants. Plant damage caused by oxidative stress during long-term Cd accumulation is well documented, while the primary response to Cd uptake is poorly understood. Objective: We assess the short-term injury and the primary reaction of the antioxidant system to the rapid accumulation of Cd in tobacco leaves. Methods: Leaf rosettes of the 5-week-old plants without roots were exposed to 100, 500 and 5000 μM Cd chloride for 2 and 12 hours. Cd accumulation, oxidation levels of proteins and lipids, content of reduced (Asc) and oxidized (DHA) ascorbate and activity of CAT, APX and POD were determined. Results: An accumulation of Cd in high concentration, but only a relatively small increase in the oxidation of proteins and lipids was found in the leaves after 2 hours of treatment. These effects were transient and disappeared after 12 hours. No visible damage to plants was observed. After 12 hours, the total ascorbate content (Asc + DHA) decreased by 18%, remained unchanged or increased by 85% after the application of 100, 500 and 5000 µM Cd chloride. The increase in the ascorbate pool, which should be considered as a component of the protective response, was caused by the accumulation of DHA. The activity of APX and POD remained unchanged, while that of CAT decreased, indicating that antioxidant enzymes activation is not involved in the primary response to Cd. Conclusions: The primary generation of ROS induced by Cd does not appear to be a deleterious manifestation of Cd toxicity, but rather a component of stress signaling that causes activation of the protective response. Uptake of Cd caused severe damage to the plant after long-term, but not after short-term treatment, suggesting that the damage is the result of secondary effects of Cd toxicity.

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“…Важливим компонентом антиоксидантної системи рослин є САТ, яка серед іншого приймає участь у захисті рослин від сольового стресу [22]. Раніше було встановлено, що залежно від умов культивування та стресової обробки суттєве зниження активності САТ у нокаутної лінії cat-2 A. thaliana частково компенсується активацією пероксидаз (АРХ та POD), ізоформи CAT3 [23], а також -збільшенням пулу глутатіону [24,25]. Наші нові дані свідчать, що перебудови у роботі антиоксидантної системи у нокаутної лінії cat2 також стосуються змін у активності DHAR, зокремаза умов сольового стресу.…”

Section: результати та обговоренняunclassified

Influence of sodium chloride on the dehydroascorbate reductase activity in Arabidopsis thaliana catalase 2 knokout mutant

Buzduga¹,

Волков²,

Панчук³

2018

Visn. ukr. tov. genet. sel.

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Aim. To better understand the mechanisms of abiotic stress resistance in plants, it is important to clarify the role of individual antioxidant enzymes from the same multiproteinic family in the response to stress. It is known that the loss of some isoforms of antioxidant enzymes can be compensated by activation of other enzymes. However, the functional interaction of the ascorbate-glutathione cycle enzymes with catalase under salt stress still remains unexplored. Respectively, we determined the activity of DHAR in knock-out mutants of Arabidopsis thaliana under salt stress. Methods. The DHAR activity was determined in the knock-out line cat2 and in wild-type (WT) Arabidopsis plants after various regimes of treatment with sodium chloride. Results. After treatment with 200 mM sodium chloride in the dark, activation of DHAR was found after 8 hours in WT plants and after 4 hours in the knock-out line cat2. However stress treatment under illumination resulted in significant increase in DHAR activity after 8 hours in both studied lines. In this case, DHAR activity in cat2 was lower than in WT, whereas in non-treated plants or upon stress treatment in the dark no difference between the tested lines was detected. Conclusions. The obtained data indicate that under salt stress conditions, changes in the DHAR activity are included into functional rearrangements of the antioxidant system in cat2 line, which compensate the loss of activity of CAT2 isoenzyme.Keywords: dehydroascorbate reductase, antioxidants, reactive oxygen species (ROS), salt stress, Arabidopsis thaliana

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Metabolic compensation in Arabidopsis thaliana catalase-deficient mutants

Cited by 11 publications

References 27 publications

Increased survival of honey bees consuming pollen and beebread is associated with elevated biomarkers of oxidative stress

Increased survival of honey bees consuming pollen and beebread is associated with elevated biomarkers of oxidative stress

Rapid Accumulation of Cadmium and Antioxidative Response in Tobacco Leaves

Influence of sodium chloride on the dehydroascorbate reductase activity in Arabidopsis thaliana catalase 2 knokout mutant

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