Metabolic conversion of aclacinomycins B and Y to A by pH shift during fermentation with Streptomyces lavendofoliae DKRS

Kim, W S; Youn, Duk Joong; Kim, H R; Rhee, Shin Hyung; Choi, Eui Sung

doi:10.1007/bf00156354

Cited by 3 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is challenging to separate ACM-A from its analogues due to similar structures. To increase ACM-A and accordingly reduce analogues, S. lavendofoliae ATCC 15872 was mutated, leading to S. lavendofoliae DKRS, which produced 125 mg/L ACM-A [ 127 ]. Adjusting the pH value at the late stage of fermentation could also promote ACM-B conversion to ACM-A and thereby facilitate the purification of ACM-A [ 128 ].…”

Section: Strategies For Improving Acm-a Productionmentioning

confidence: 99%

“…To increase ACM-A and accordingly reduce analogues, S. lavendofoliae ATCC 15872 was mutated, leading to S. lavendofoliae DKRS, which produced 125 mg/L ACM-A [ 127 ]. Adjusting the pH value at the late stage of fermentation could also promote ACM-B conversion to ACM-A and thereby facilitate the purification of ACM-A [ 128 ]. Interestingly, the mutants of S. galilaeus H026 caused by the treatment of N-methyl-N′-nitro-N-nitrosoguanidine (NTG) could convert AcmB to AcmA, but could not catalyze AcmA to AcmB, which is conducive to the industrial production of AcmA [ 129 ].…”

Section: Strategies For Improving Acm-a Productionmentioning

confidence: 99%

See 1 more Smart Citation

Rethinking Biosynthesis of Aclacinomycin A

Tian

2023

Molecules

View full text Add to dashboard Cite

Aclacinomycin A (ACM-A) is an anthracycline antitumor agent widely used in clinical practice. The current industrial production of ACM-A relies primarily on chemical synthesis and microbial fermentation. However, chemical synthesis involves multiple reactions which give rise to high production costs and environmental pollution. Microbial fermentation is a sustainable strategy, yet the current fermentation yield is too low to satisfy market demand. Hence, strain improvement is highly desirable, and tremendous endeavors have been made to decipher biosynthesis pathways and modify key enzymes. In this review, we comprehensively describe the reported biosynthesis pathways, key enzymes, and, especially, catalytic mechanisms. In addition, we come up with strategies to uncover unknown enzymes and improve the activities of rate-limiting enzymes. Overall, this review aims to provide valuable insights for complete biosynthesis of ACM-A.

show abstract

Section: Strategies For Improving Acm-a Productionmentioning

confidence: 99%

Section: Strategies For Improving Acm-a Productionmentioning

confidence: 99%

Rethinking Biosynthesis of Aclacinomycin A

Tian

2023

Molecules

View full text Add to dashboard Cite

show abstract

Novel aromatic polyketides from soil Streptomyces spp.: purification, characterization and bioactivity studies

Bundale

Begde

Pillai

et al. 2018

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Aromatic polyketides are important therapeutic compounds which include front line antibiotics and anticancer drugs. Since most of the aromatic polyketides are known to be produced by soil dwelling Streptomyces, 54 Streptomyces strains were isolated from the soil samples. Five isolates, R1, B1, R3, R5 and Y8 were found to be potent aromatic polyketide producers and were identified by 16S rRNA gene sequencing as Streptomyces spectabilis, Streptomyces olivaceus, Streptomyces purpurascens, Streptomyces coeruleorubidus and Streptomyces lavendofoliae respectively. Their sequences have been deposited in the GenBank under the accession numbers KF468818, KF681280, KF395224, KF527511 and KF681281 respectively. The Streptomyces strains were cultivated in the media following critically optimised culture conditions. The resulting broth extracts were fractionated on a silica gel column and preparative TLC to obtain pure compounds. The pure compounds were tested for bioactivity and the most potent compound from each isolate was identified by UV-Vis, IR and NMR spectroscopic methods. Isolated S. spectabilis (R1), yielded one potent compound identified as dihydrodaunomycin with an MIC of 4 µg/ml against Bacillus cereus and an IC value of 24 µM against HeLa. S. olivaceus (B1), yielded a comparatively less potent compound, elloramycin. S. purpurascens (R3) yielded three compounds, rhodomycin, epelmycin and obelmycin. The most potent compound was rhodomycin with an MIC of 2 µg/ml against B. cereus and IC of 15 µM against HeLa. S. coeruleorubidus (R5), yielded daunomycin showing an IC of 10 µM and also exhibiting antimetastatic properties against HeLa. S. lavendofoliae (Y8), yielded a novel aclacinomycin analogue with IC value of 2.9 µM and potent antimetastatic properties at 1 µM concentration against HeLa. The study focuses on the characterization of aromatic polyketides from soil Streptomyces spp., which can serve as potential candidates for development of chemotherapeutic drugs in future.

show abstract

Chitosan-Based Bioactive Formulations for the Control of Powdery Mildew in Viticulture

et al. 2022

View full text Add to dashboard Cite

Grapevine is highly susceptible to fungal diseases, whose incidence and severity increase due to climate change. The present work focuses on the assessment of eight combinations of natural products with chitosan oligomers with fungicidal capacity that may be effective in the integrated control of powdery mildew, in compliance with Article 14 of the European Directive 2009/128/EC. Their efficacy was evaluated in field conditions against natural infections, in a plot with high disease pressure during a growing season (assaying both foliar or root application), and against overwintering inoculums (chasmothecia) through in vitro tests. In addition, their possible biostimulant capacities were evaluated based on harvest yields. Treatments based on chitosan oligomers in combination with secondary metabolites of Streptomyces spp. and chitosan oligomers combined with hydrolyzed gluten showed the best results in terms of disease control. Given the high efficacy of these formulations, comparable to that of conventional antifungals, they constitute an interesting alternative for the control of this disease whose treatment can, in some cases, represent almost half of the production costs.

show abstract

Metabolic conversion of aclacinomycins B and Y to A by pH shift during fermentation with Streptomyces lavendofoliae DKRS

Cited by 3 publications

References 6 publications

Rethinking Biosynthesis of Aclacinomycin A

Rethinking Biosynthesis of Aclacinomycin A

Novel aromatic polyketides from soil Streptomyces spp.: purification, characterization and bioactivity studies

Chitosan-Based Bioactive Formulations for the Control of Powdery Mildew in Viticulture

Contact Info

Product

Resources

About