Metabolic differentiation of Arabidopsis treated with methyl jasmonate using nuclear magnetic resonance spectroscopy

Hendrawati, Oktavia; Yao, Qingqiang; Kim, Hye Kyong; Linthorst, Huub J. M.; Erkelens, C.; Lefeber, Alfons W.M.; Choi, Young Hae; Verpoorte, Robert

doi:10.1016/j.plantsci.2006.01.017

Cited by 76 publications

(66 citation statements)

References 22 publications

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“…The primary metabolites, malic and citric acid were found in the present study to be altered in both locally inoculated and systemically -infected tomato leaves. Our results are consistent with those of Choi et al (2006), studying the hypersensitive interaction between 370 tobacco and tobacco mosaic virus (TMV). The authors reported that these organic acids also accumulated in both TMV-inoculated and in SAR tobacco leaves.…”

supporting

confidence: 87%

“…In this work, tomato leaf metabolites were extracted using a single-step extraction method consisting of a polar solvent mixture of methanol and a buffered aqueous solution. This method is practical, is not time consuming and 250 has been shown to possess a high extraction efficiency constitutes an optimized extraction protocol for polar untargeted metabolites (Hendrawati et al, 2006;Verpoorte et al, 2007;Verpoorte et al, 2008). NMR spectroscopy in combination with multivariable data analysis was performed on control and ToMV-infected tomato leaves in order to determine the metabolic changes during this plant-pathogen interaction.…”

Section: Plant Metabolites Have Very Diverse Physical-chemical Propermentioning

confidence: 99%

“…The first step of NMR metabolomic studies is the analysis of the plant extract spectra (Choi et al, 2006). 1 H NMR spectra, 2D NMR experiments (2D J-resolved, COSY and HMBC), our in-house database of reference compounds and previous reports (López-Gresa et al, 2010) were used for the signal assignment of metabolites from the tomato leaves.…”

Section: Plant Metabolites Have Very Diverse Physical-chemical Propermentioning

confidence: 99%

“…Although it is impossible for one single analytical method to completely characterize the entire plant metabolite profile, nuclear magnetic resonance spectroscopy (NMR) is an excellent tool to provide a macroscopic view of the majority of the components of the plant 100 metabolome Broyart et al, 2010;Leiss et al, 2011). In fact, NMR-based metabolomics has been widely used to decode the broad range of chemical compounds that might be implicated in the plant defence against microbe attacks (Choi et al, 2006;Ward et al, 2010). Our group has been applying this technology to the detection and structure elucidation of metabolites in plants upon 105 treatment with a wide range of biotic and abiotic stress agents (Choi et al, 2004;Simoh et al, 2009;Jahangir et al, 2009;Mirnezhad et al, 2010;Simoh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…However, few metabolomic reports on plant/virus interactions have been described in the literature, and a complete analysis of metabolites has been carried out only in the case of the hypersensitive response of tobacco plants to tobacco mosaic virus (TMV) 120 (Choi et al, 2006). In our previous report, some characteristic metabolites of tomato plants related to either Citrus Exocortis viroid (CEVd) or Pseudomonas syringae infection have been identified by NMR-based metabolomics (López-Gresa et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

López-Gresa

Lisón

Kim

et al. 2012

Journal of Plant Physiology

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general time-dependent decrease in organic acids, amino acids (excluding asparagine), phenylpropanoids and rutin was observed in individual leaves. In addition, metabolite alterations were also found to correlate with the developmental stage of the leaf: high levels of organic acids, some amino acids, phenylpropanoids, and flavonoids were found in lower leaves while elevated amounts of sugars were present in the upper ones. 35Moreover, a marked variation in the content of some metabolites was also observed to be associated to the asymptomatic ToMV infection both in inoculated and systemically infected leaves. While flavonoids accumulated in virus-inoculated leaves, increased levels of phenylpropanoids were observed in non-inoculated leaves where ToMV actively replicates. Finally, diurnal changes in the metabolite content were also 40 observed: an increase of amino acids and organic acids (except glutamic acid) were observed in the samples collected in the morning, whereas sugars and secondary metabolite levels increased in the tomato leaves harvested in the evening.

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supporting

confidence: 87%

Section: Plant Metabolites Have Very Diverse Physical-chemical Propermentioning

confidence: 99%

Section: Plant Metabolites Have Very Diverse Physical-chemical Propermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

López-Gresa

Lisón

Kim

et al. 2012

Journal of Plant Physiology

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A Role for Metabolomics in Plant Ecology

Dam

Meijden

2018

Annual Plant Reviews Online

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Plant metabolomic analyses have been mostly applied to analyse metabolomes of crops and model species. However, metabolomic analyses may also be extremely valuable for plant ecologists. Because of the untargeted nature of most metabolomic platforms, the analyses will provide a comprehensive overview of both the primary and secondary plant metabolome. This is especially valuable for chemical ecologists studying plant–herbivore interactions, as both primary and secondary metabolites together determine the performance and preference of herbivores feeding on a plant. A few examples from the recent scientific literature exemplify how metabolomics may yield novel knowledge on the effects of hybridization on plant chemistry, insect resistance and genotype × environment interactions that could not have been so easily obtained with targeted studies alone. Theories and hypotheses regarding optimal defence strategies in plants and invasive plant biology may also benefit from metabolomic analysis. Testable hypotheses built on our current knowledge of plant responses to herbivores and competition may be substantiated and expanded based on metabolomic analyses. Despite some of the limitations that metabolomics analyses may have, the implementation of broad‐spectrum chemical analysis in plant ecology will definitively open up new horizons for plant ecologists and enhance our understanding of the importance of plant chemistry in plant–environment interactions for fundamental and applied research.

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Metabolomics of A rabidopsis Thaliana

Sussman

2018

Annual Plant Reviews Online

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Since the publication of the complete genome sequence of the model plant Arabidopsis thaliana, the precision annotation of the exact function of each gene has become a major research activity. Metabolomic analysis has much to offer in this endeavour and the small physical size and generation time of Arabidopsis make it a particularly attractive system for large‐scale metabolomic data collection and analysis. In this review, we summarize the known phytochemistry of Arabidopsis, with particular emphasis on recent discoveries that indicate that the complexity of this plant's secondary metabolome is much greater than originally realized. We survey how the different analytical technologies, used in metabolomic analysis, are applied to quantify both primary and secondary metabolites of Arabidopsis. The use of isotope labelling to derive empirical formulae for unknown metabolites is highlighted, as is co‐expression analysis of transcriptome and metabolome data, as both of these technologies are well suited to this species. The application of the various techniques to study the molecular plant physiology, genetics and functional genomics of Arabidopsis is reviewed, with particular reference to the use of mutants and other genetic resources, such as recombinant inbred lines.

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Metabolic differentiation of Arabidopsis treated with methyl jasmonate using nuclear magnetic resonance spectroscopy

Cited by 76 publications

References 22 publications

Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

A Role for Metabolomics in Plant Ecology

Metabolomics of A rabidopsis Thaliana

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