Metabolic differentiations of dwarf elder by NMR-based metabolomics

Zahmanov, Georgi; Alipieva, Kalina; Simova, Svetlana; Georgiev, Milen I.

doi:10.1016/j.phytol.2014.11.021

Cited by 35 publications

(31 citation statements)

References 22 publications

(43 reference statements)

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“…The spectra can be divided into two regions: (i) aliphatic region where the resonances of primary metabolites, such as sugars and organic acids dominate and (ii) aromatic region where the resonances are attributed to the secondary metabolites, such as phenolic compounds. The 1 H-NMR resonances of hydrophilic metabolites were assigned based on comparison with chemical shifts of standard compounds as well as previously published data ( Gall et al, 2003 ; Tarachiwin et al, 2008 ; Kim et al, 2011 ; Capitani et al, 2013 ; Zahmanov et al, 2015 ). The most diagnostic resonances for the assignment of major metabolites in sweet fruits are illustrated in Figure 8B .…”

Section: Resultsmentioning

confidence: 99%

1H NMR Metabolic Fingerprinting to Probe Temporal Postharvest Changes on Qualitative Attributes and Phytochemical Profile of Sweet Cherry Fruit

et al. 2015

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Sweet cherry fruits (Prunus avium cvs. ‘Canada Giant’, ‘Ferrovia’) were harvested at commercial maturity stage and analyzed at harvest and after maintenance at room temperature (storage at ∼20°C, shelf life) for 1, 2, 4, 6, and 8 days, respectively. Fruit were initially analyzed for respiration rate, qualitative attributes and textural properties: ‘Canada Giant’ fruit were characterized by higher weight losses and stem browning index, being more intense over the late stages of shelf life period; meanwhile ‘Ferrovia’ possessed appreciably better performance even after extended shelf life period. A gradual decrease of respiration rate was monitored in both cultivars, culminated after 8 days at 20°C. The sweet cherry fruit nutraceutical profile was monitored using an array of instrumental techniques (spectrophotometric assays, HPLC, 1H-NMR). Fruit antioxidant capacity was enhanced with the progress of shelf life period, concomitant with the increased levels of total anthocyanin and of phenolic compounds. ‘Ferrovia’ fruit presented higher contents of neochlorogenic acid and p-coumaroylquinic acid throughout the shelf life period. We further developed an 1H-NMR method that allows the study of primary and secondary metabolites in a single running, without previous separation and isolation procedures. Diagnostic peaks were located in the aliphatic region for sugars and organic acids, in the aromatic region for phenolic compounds and at 8.2–8.6 ppm for anthocyanins. This NMR-based methodology provides a unifying tool for quantitative and qualitative characterization of metabolite changes of sweet cherry fruits; it is also expected to be further exploited for monitoring temporal changes in other fleshy fruits.

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Section: Resultsmentioning

confidence: 99%

1H NMR Metabolic Fingerprinting to Probe Temporal Postharvest Changes on Qualitative Attributes and Phytochemical Profile of Sweet Cherry Fruit

et al. 2015

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“…Applied as a holistic approach in order to distinguish the possible therapeutic agents in herbal medicine, up to date, this technique is implemented in the assessment and generation of standardized biomarkers of pharmacologically active molecules essential to ensure the quality, safety and reproducibility of the natural products (Cerulli et al, 2018;Deborde et al, 2017). In our research group, NMR-based profiling and metabolomics have been intensively applied towards identifying specific marker compounds in wide variety of medicinal plant species (Georgiev et al, 2015;Marchev et al, 2017aMarchev et al, , 2017bZahmanov et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Clinopodium vulgare L. (wild basil) extract and its active constituents modulate cyclooxygenase-2 expression in neutrophils

Amirova

Dimitrova

Marchev

et al. 2019

Food and Chemical Toxicology

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Clinopodium vulgare L. (wild basil) has a wide range of ethnopharmacological applications and accumulates a broad spectrum of phenolic compounds, recognized for their anti-inflammatory and anticancer properties. The triggered cyclooxygenase-2 (COX-2) expression is creating an immunosuppressive microenvironment in the inflamed tissue and considered to be the main cause of failure of even new anticancer-/immune-therapies. Nowadays, selective and novel plant-derived COX-2 inhibitors with safe profile are subject of profound research interest. This study aimed to analyze the metabolic profile of C. vulgare and search for phenolic molecules with potential biological properties. By application of 1 H and 2D-NMR (Nuclear Magnetic Resonance) profiling, caffeic, chlorogenic acids and catechin were identified along with a bunch of primary and secondary metabolites. Further, the biological effect of C. vulgare extract (CVE) and its constituents on zymosan-induced COX-2 expression and apoptosis of murine neutrophils have been studied. The CVE, caffeic and chlorogenic acids inhibited zymosan-induced COX-2 expression in bone marrow neutrophils, in vitro and in vivo activated. The obtained data indicate that CVE may have a good potential to manipulate neutrophil functions, however, its action may depend on the cellular state, the inflammatory milieu and the relative content of caffeic and chlorogenic acid in the extract.

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“…In most cases, therapeutic activities and sometimes toxic effects of herbal medicines are derived from synergistic actions of multiple secondary metabolites in a single or among multiple herbs used in the formulation (Georgiev et al, 2011;Kim et al, 2010a;Razmovski-Naumovski et al, 2010;Zahmanov et al, 2015). Metabolomics approach offers a new hope towards standardization and efficacy validation of the herbal and complementary medicines, while at the same time strengthens its public acceptance (Buriani et al, 2012;Yamazaki et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Nigella sativa seeds from four different origins and their bioactivity correlations based on NMR-metabolomics approach

Maulidiani

Sheikh

Mediani

et al. 2015

Phytochemistry Letters

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Metabolic differentiations of dwarf elder by NMR-based metabolomics

Cited by 35 publications

References 22 publications

1H NMR Metabolic Fingerprinting to Probe Temporal Postharvest Changes on Qualitative Attributes and Phytochemical Profile of Sweet Cherry Fruit

1H NMR Metabolic Fingerprinting to Probe Temporal Postharvest Changes on Qualitative Attributes and Phytochemical Profile of Sweet Cherry Fruit

Clinopodium vulgare L. (wild basil) extract and its active constituents modulate cyclooxygenase-2 expression in neutrophils

Differentiation of Nigella sativa seeds from four different origins and their bioactivity correlations based on NMR-metabolomics approach

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