Metabolic disease prevention and suppression of fat accumulation by Salacia reticulata

Shimada, Tsutomu; Nagai, Eiichi; Harasawa, Yukiko; Akase, Tomoko; Aburada, Takashi; Iizuka, Seiichi; Miyamoto, Ken‐ichi; Aburada, Masaki

doi:10.1007/s11418-010-0401-1

Cited by 19 publications

(17 citation statements)

References 26 publications

(54 reference statements)

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“…Maxim. (Asteraceae)Flower BudEthanol extractInhibition of expression of PPARγ, C/EBPα, and SREBP-1c[90] Salacia reticulata Wight (Celastraceae)WholeAqueous extractInhibition of expression of PPARγ, C/EBPα, and GPDH[91, 92] Vigna nakashimae (Fabaceae) SeedEthanol extractInhibition of expression of PPARγ via activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK)[93] Vitis vinifera (L.) (Vitaceae)SeedVitisin AInhibition of expression of PPARγ, and C/EBPα[94] Wasabia japonica (Miq.) Matsum.…”

Section: Plants Investigated For Anti-lipid Droplets Accumulation Actmentioning

confidence: 99%

Plant natural products as an anti-lipid droplets accumulation agent

2014

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Recently people often suffer from unhealthy energy metabolism balance as they tend to take more energy than required. Normally, excess energy taken in is converted into triglyceride and stored in adipocyte as lipid droplets. Recent studies have suggested that irregular accumulation of triglyceride in adipocyte might be a cause of many metabolic diseases. Thus, the awareness of the detrimental effects on health of excessive lipid droplets accumulation (LDA) has urged the development or finding of drugs to counter this effect, including those from botanical origins. This review summarized recent progress in this field from the viewpoint of crude drug studies with references to their anti-LDA activity. Possible mechanisms involved in their anti-LDA effect and isolations of the relevant bioactive compounds were also discussed.

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Section: Plants Investigated For Anti-lipid Droplets Accumulation Actmentioning

confidence: 99%

Plant natural products as an anti-lipid droplets accumulation agent

2014

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“…In traditional Sri Lankan medicine, called ‘Ayurveda’, the roots and stems of SR are used for the prevention of rheumatism and diabetes [1,2]. For example, the roots and stems of SR are known to contain unique compounds such as salacinol, kotalanol, and mangiferin [3-5].…”

Section: Resultsmentioning

confidence: 99%

Anti-proliferative effects of Salacia reticulata leaves hot-water extract on interleukin-1β-activated cells derived from the synovium of rheumatoid arthritis model mice

Sekiguchi¹,

Miyata²,

Nakatani³

et al. 2012

BMC Res Notes

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BackgroundSalacia reticulata (SR) is a plant native to Sri Lanka. In ayurvedic medicine, SR bark preparations, taken orally, are considered effective in the treatment of rheumatism and diabetes. We investigated the ability of SR leaves (SRL) to inhibit in vitro the interleukin-1β (IL-1β)-activated proliferation of synoviocyte-like cells derived from rheumatoid arthritis model mice.FindingsInflammatory synovial tissues were harvested from type II collagen antibody-induced arthritic mice. From these tissues, a synoviocyte-like cell line was established and named MTS-C H7. To determine whether SRL can suppress cell proliferation and gene expression in MTS-C H7 cells, fractionation of the SRL hot-water extract was performed by high-performance liquid chromatography (HPLC), liquid-liquid extraction, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and protease digestion.The 50% inhibitory concentration of the SRL hot-water extract against MTS-C H7 cells proliferation was ~850 μg/mL. Treatment with a low dose (25 μg dry matter per millilitre) of the extract inhibited IL-1β-induced cell proliferation and suppressed the expression of the matrix metalloproteinase (MMP) genes in MTS-C H7 cells. Various polyphenolic fractions obtained from HPLC and the fractions from liquid-liquid extraction did not affect cell proliferation. Only the residual water sample from liquid-liquid extraction significantly affected cell proliferation and the expression of MMP genes. The results of SDS-PAGE and protease digestion experiment showed that low molecular weight proteins present in SRL inhibited the IL-1β-activated cell proliferation.ConclusionsWe surmised that the residual water fraction of the SRL extract was involved in the inhibition of IL-1β-activated cell proliferation and regulation of mRNA expression in MTS-C H7 cells. In addition, we believe that the active ingredients in the extract are low molecular weight proteins.

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“…23,24) Geniposide showed preventative effects on various metabolic disease symptoms in the TSOD mice. The body weight increase seen in the TSOD mice was significantly suppressed by geniposide from the 5th week after starting the treatment.…”

Section: Discussionmentioning

confidence: 98%

Preventive Effect of Geniposide on Metabolic Disease Status in Spontaneously Obese Type 2 Diabetic Mice and Free Fatty Acid-Treated HepG2 Cells

Kojima

Shimada

Nagareda

et al. 2011

Biological & Pharmaceutical Bulletin

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Obesity, diabetes, hyperlipidemia and hypertension, which are caused by hypernutrition and lack of exercise, are often found in the same person. These symptoms used to be called "the deadly quartet," "Syndrome X" or "insulin resistance syndrome," but these names have recently been collectively replaced with "metabolic syndrome (MS)," since the presence of visceral fat accumulation-type obesity has been recognized as the major disease base. It should be noted that progression of MS increases the risk of future onset of ischemic cardiac diseases and cerebrovascular diseases, and the importance of "prevention" and "early therapy" of MS is emphasized.Gardeniae fructus, the fruit of Gardenia jasminoides ELLIS, is widely used in Asian countries as a natural colorant, and it is also used as a Japanese and Chinese traditional medicine since it has a homeostatic effect, an antiphlogistic effect, an analgesic effect, an antipyretic effect, a hepatoprotective effect and a hypolipidaemic effect.1,2) Geniposide, an iridoid glycoside contained in Gardeniae fructus, is reported to have a hepatoprotective effect, an antioxidant effect, a hypoglycemic effect, a vascular endothelial cell adhesion-suppressing effect, an anti-lipopolysaccharide (LPS) effect, a neuroprotective effect, and an anti-inflammatory effect. [3][4][5][6][7][8][9] In addition, genipin, which is the aglycone portion of geniposide and is formed as a metabolite, is also reported to have an anti-inflammatory effect, a bile secretion-enhancing effect, and an anti-thrombotic effect. [10][11][12] Geniposide is promising as a seed compound for developing therapeutic medicines for metabolic diseases. However, no publication has reported the effect of geniposide on various metabolic disease symptoms based on visceral fat accumulation. TSOD mice are model animals showing spontaneously multifactorial genetic Type 2 diabetes based on visceral fat accumulation. [13][14][15][16][17][18] Since the cause of onset and the symptoms are very similar to those of human MS, this model is regarded as promising for the study of MS. In the present study, we investigated the effect of geniposide on various metabolic disease symptoms based on visceral fat accumulation using TSOD mice. In addition, a fatty liver model was prepared by adding a free fatty acid to human liver cancer cells (HepG2 cells), 19) and the direct effect of genipin on the liver was investigated. MATERIALS AND METHODSReagents Geniposide and genipin (Fig. 1) were supplied by Tsumura and Co. (Ibaraki, Japan). Palmitic acid powder was purchased from Sigma-Aldrich (MO, U.S.A.). The other reagents used were of the highest grade available.Animal Experiments Experimental Animals: Male TSOD mice and corresponding control animals (TSNO mice) were purchased from the Institute for Animal Reproduction (Ibaraki) at the age of 3 weeks. They were acclimated under controlled circumstances (temperature: 23Ϯ1°C, humidity: 55Ϯ5%, lighting hours: 12 h) for one week. During the acclimation period, the animals were given ordinary powder ...

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Metabolic disease prevention and suppression of fat accumulation by Salacia reticulata

Cited by 19 publications

References 26 publications

Plant natural products as an anti-lipid droplets accumulation agent

Plant natural products as an anti-lipid droplets accumulation agent

Anti-proliferative effects of Salacia reticulata leaves hot-water extract on interleukin-1β-activated cells derived from the synovium of rheumatoid arthritis model mice

Preventive Effect of Geniposide on Metabolic Disease Status in Spontaneously Obese Type 2 Diabetic Mice and Free Fatty Acid-Treated HepG2 Cells

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