Metabolic disposition of cephalothin and deacetylcephalothin in children and adults: Comparison of high‐performance liquid chromatographic and microbial assay procedures

Renal excretion of cephapirin and cephaloridine: Evidence for saturable tubular reabsorption

“…Significant differences have been reported between the latter and the more specific HPLC due to the fact that antibacterially active metabolites can interfere in the microbiological methods. 12 In our investigation on cephapirin kinetics, the use of a specific assay was crucial, since the drug is deacetylated to form a microbiologically active metabolite. 7…”

Pharmacokinetics of β-Lactam Antibiotics

“…Several methods are available for the determination of cephalothin and its metabolite in biological fluids (MILLER 1962;HOEHN et al 1970;COOPER et al 1973). In healthy volunteers and patients with normal kidney function given cephalothin sodium intravenously, cephalothin serum levels declined with a mean serum halflife of 11.8-13.5 min (COOPER et al 1973;ROLEWICZ et al 1977). In subsequent studies by ANDERS et al (1975) and ROLEWICZ et al (1977), pediatric subjects received cephalothin sodium intravenously and again a very short mean terminal serum half-life was noted (14.9-18.6 min).…”

“…In healthy volunteers and patients with normal kidney function given cephalothin sodium intravenously, cephalothin serum levels declined with a mean serum halflife of 11.8-13.5 min (COOPER et al 1973;ROLEWICZ et al 1977). In subsequent studies by ANDERS et al (1975) and ROLEWICZ et al (1977), pediatric subjects received cephalothin sodium intravenously and again a very short mean terminal serum half-life was noted (14.9-18.6 min). Using a nondiscriminating biological assay, the apparent half-life in adult serum was 1.7 times greater (ROLEWICZ et al 1977).…”

“…In subsequent studies by ANDERS et al (1975) and ROLEWICZ et al (1977), pediatric subjects received cephalothin sodium intravenously and again a very short mean terminal serum half-life was noted (14.9-18.6 min). Using a nondiscriminating biological assay, the apparent half-life in adult serum was 1.7 times greater (ROLEWICZ et al 1977). Urine collections through 5 h in this study accounted for a mean of 102% of the administered dose consisting of 35.7% desacetylcephalothin and 66.3% cephalothin.…”

Polarographic behavior of cephalexin and its determination in pharmaceuticals and human serum

“…A great variety of methods to determine cephalexin have been reported over these years, including chromatography [1][2][3], spectrophotometry [4][5][6][7], a fluorescence method [8], a flow-through immunoanalysis [9] and electroanalytical methods [10][11][12][13][14][15][16][17][18][19][20][21][22].…”