Metabolic Elasticity and Induction of Heat Shock Protein 70 in Labeo rohita Acclimated to Three Temperatures

Das, T. K.; Pal, A. K.; Chakraborty, S. K.; Manush, S.M.; Chatterjee, Nirupama

doi:10.5713/ajas.2006.1033

Cited by 41 publications

(22 citation statements)

References 39 publications

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“…The AST and ALT enzyme activities increased with the heat shock, which in turn converts amino acid to an intermediate, which can enter TCA cycle and finally produce ATP to cope up with the stress. Similar results were also reported by Das et al (2006) in L. rohita exposed to different temperatures and by Vijayan et al (1997) in tilapia exposed to confinement stress.…”

Section: Discussionsupporting

confidence: 92%

“…In the present study, there was a significant increase in MDH activity in fishes after heat shock, which strengthens the above hypothesis. This is supported by the findings of Das et al (2006), who found elevated MDH activity in fishes acclimated at higher temperature.…”

Section: Discussionsupporting

confidence: 78%

“…thermal stress (Grigo 1975), starvation stress (Vijayaraghavan and Rao 1986) and confinement stress . Das et al (2006) also reported that LDH activity significantly increased at higher temperature in L. rohita. Lower activity in the high protein-fed groups compared to low protein-fed groups after heat shock suggested that the high dietary protein helps in reducing stress in the L. rohita.…”

Section: Discussionmentioning

confidence: 91%

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High dietary protein combats the stress of Labeo rohita fingerlings exposed to heat shock

Kumar

Sahu

Pal

et al. 2011

Fish Physiol Biochem

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The amelioration effect of high dietary protein against stress was evaluated in Labeo rohita fingerlings, exposed to heat shock. Two hundred and forty fingerlings (6.57 ± 0.04 g, average weight ± SE) were randomly distributed into 4 treatment groups, each with 4 replicates was fed with either of four diets containing different levels of protein (20, 30, 40 or 45%). Water temperatures of all the treatments were within the range of 25.5-26.5°C throughout the experimental period of 30 days. After 30 days of feeding, fish were given heat shock by exposing to 38°C for 2 h. Heat shock significantly decreased (P < 0.05) liver glycogen content in treatment groups fed with 20 and 30% dietary protein, whereas unaffected in the 40 and 45% protein-fed groups. Heat shock significantly increased (P < 0.05) serum glucose and cortisol level in all the treatments. The 40 and 45% dietary protein-fed groups registered significantly higher survival (%) after the heat shock compared with their lower-protein counterparts. Heat shock increased the glycolytic, gluconeogenic, protein metabolic and antioxidative enzymes to cope up with thermal stress. Our results indicate that high-protein diet (≥40%) combats the stress due to heat shock in Labeo rohita.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 91%

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High dietary protein combats the stress of Labeo rohita fingerlings exposed to heat shock

Kumar

Sahu

Pal

et al. 2011

Fish Physiol Biochem

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“…Medaka (Orizias latipes) embryos were unable to induce stress protein synthesis in earlier stages and were more susceptible to heat shock than embryos at later stages where HSP induction was observed (Werner et al 2001). Das et al (2006) reported an increase in the inducible form of HSP70 in the liver tissue of L. rohita acclimated to three temperatures (31, 33 and 36°C) and suggested that the increased level of HSP70 was responsible for the increase in thermal tolerance with increasing acclimation temperatures in L. rohita . Similarly, the increase in the thermal tolerance with increasing acclimation temperature observed in the present study suggests that the increase in the HSP70 renders H. brachysoma more thermotolerant with increasing acclimation temperature.…”

Section: Discussionmentioning

confidence: 96%

Influence of acclimation temperature on the induction of heat-shock protein 70 in the catfish Horabagrus brachysoma (Günther)

Dalvi

Pal

Tiwari

et al. 2011

Fish Physiol Biochem

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Every organism responds to heat stress by synthesizing a group of evolutionarily conserved proteins called the heat-shock proteins (HSPs) that, by acting as molecular chaperones, protect the cell against the aggregation of denatured proteins and play a significant role in adaptation to temperature. The present study aimed to investigate the critical thermal maxima (CTMax) and the expression of HSP70 in different tissues (gill, brain, muscle and liver) of an endemic catfish Horabagrus brachysoma acclimated at either 20 or 30°C for 30 days. To understand the HSP70 response, fish acclimated to the two temperatures were exposed to preset temperatures (26, 30, 34, 36 and 38°C for 20°C acclimated fish and 32, 34, 36, 38 and 40°C for 30°C acclimated fish) for 2 h, followed by 1 h recovery at their respective acclimation temperatures. The HSP70 levels in the gill, brain, muscle and liver tissues were determined by Western blotting of one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A significant (P < 0.05) increase in the CTMax values was observed for fish acclimated at 30°C (41.86 ± 0.39°C) than those acclimated at 20°C (39.13 ± 0.18°C). HSP70 was detected in all the tissues with the highest level in the liver followed by intermediate levels in muscle and brain, and lowest level in gill tissue, irrespective of the acclimation temperatures (20 or 30°C). The HSP70 levels were significantly higher (P < 0.05) in the tissues of fish acclimated at 30°C than those acclimated at 20°C. The mean induction temperature of HSP70 in all the tissues of fish acclimated at either 20 or 30°C was 30 and 34°C, respectively. The optimum temperature for HSP70 induction in all the tissues of fish acclimated at 20°C was 36°C, whereas for fish acclimated at 30°C was 36°C for gill and 38°C for brain, muscle and liver. Decreased levels of HSP70 were noted in all the tissues of fish when exposed to temperatures that exceeded the optimum temperatures for HSP70 inductions. Overall results indicated that acclimation temperature influences both temperature tolerance and induction of HSP70 in H. brachysoma.

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“…Therefore, expression of Hsp genes during A. hydrophila served a dual response to infection and stress. The modulation of Hsp70 was investigated in rohu during temperature change (Das et al 2006) and starvation (Yengkokpam et al 2008). However, no study has yet revealed the modulation of this or any other genes of this family during bacterial infection in rohu.…”

Section: Introductionmentioning

confidence: 99%

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Das

Mohapatra

Sahoo

2015

Cell Stress and Chaperones

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Metabolic Elasticity and Induction of Heat Shock Protein 70 in Labeo rohita Acclimated to Three Temperatures

Cited by 41 publications

References 39 publications

High dietary protein combats the stress of Labeo rohita fingerlings exposed to heat shock

High dietary protein combats the stress of Labeo rohita fingerlings exposed to heat shock

Influence of acclimation temperature on the induction of heat-shock protein 70 in the catfish Horabagrus brachysoma (Günther)

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

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