Metabolic engineering for increased lipid accumulation in Yarrowia lipolytica – A Review

Wang, Jinpeng; Ledesma-Amaro, Rodrigo; Wei, Yongjun; Ji, Boyang; Ji, Xiao Jun

doi:10.1016/j.biortech.2020.123707

Cited by 159 publications

(91 citation statements)

References 126 publications

(113 reference statements)

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“…The lipid content and lipid production of Y. lipolytica Po1g grown in sugarcane bagasse hydrolysate were 58.5% and 6.68 g/L, respectively (Wang et al, 2020). Moreover, R. toruloides, L. starkeyi, and Y. lipolytica can produce lipid with the hydrolysate derived from the pretreatment of wheat straw (Yu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

Wang

et al. 2021

Preprint

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BackgroundRaw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates have not been introduced as feedstocks for oleaginous yeasts. ResultsIn this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing galactose-based carbohydrates, with lipid productions of 5.30 g/L and 5.23 g/L, respectively. Over 80% of the fatty acids was C16:0, C18:0, C18:1, and C18:2. All kinds of single sugar components in the two byproducts were readily converted into lipids, with yields ranging between 0.116 g/g and 0.138 g/g. Three α-galactosidases and five β-galactosidases in the strain were cloned and analyzed. β-galactosidase was responsible for lactose hydrolysis; sucrase and α-galactosidase both contributed to the efficient hydrolysis of raffinose and stachyose in a cooperation manner. ConclusionsThis is a new way to produce lipids from raw materials containing galactose-based carbohydrates. This finding revealed the significance of sucrase in the direct hydrolysis of galactose-based carbohydrates in raw materials for the first time and facilitated the understanding of the efficient utilization of galactose-based carbohydrates to manufacture lipid or other chemicals in bioprocess

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Section: Discussionmentioning

confidence: 99%

Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

Wang

et al. 2021

Preprint

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“…Other approaches focus on increasing cytoplasmic acetyl-CoA pool [50], redox engineering [51] and modulating cellular oxidative stress defense pathways [52]. Comprehensive reviews on the subject are available elsewhere [34,53,54].…”

Section: Enhancing Lipogenesis For Production Of Highvalue Lipidsmentioning

confidence: 99%

Advances in production of high-value lipids by oleaginous yeasts

Szczepańska

Hapeta

Lazar

2021

Critical Reviews in Biotechnology

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The global market for high-value fatty acids production, mainly omega-3/6, hydroxy fatty-acids, waxes and their derivatives, has seen strong development in the last decade. The reason for this growth was the increasing utilization of these lipids as significant ingredients for cosmetics, food and the oleochemical industries. The large demand for these compounds resulted in a greater scientific interest in research focused on alternative sources of oil production -among which microorganisms attracted the most attention. Microbial oil production offers the possibility to engineer the pathways and store lipids enriched with the desired fatty acids. Moreover, costly chemical steps are avoided and direct commercial use of these fatty acids is available. Among all microorganisms, the oleaginous yeasts have become the most promising hosts for lipid production -their efficient lipogenesis, ability to use various (often highly affordable) carbon sources, feasible large-scale cultivations and wide range of available genetic engineering tools turns them into powerful micro-factories. This review is an in-depth description of the recent developments in the engineering of the lipid biosynthetic pathway with oleaginous yeasts. The different classes of valuable lipid compounds with their derivatives are described and their importance for human health and industry is presented. The emphasis is also placed on the optimization of culture conditions in order to improve the yield and titer of these valuable compounds. Furthermore, the important economic aspects of the current microbial oil production are discussed.

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“…The adaptive laboratory evolution might help to increase strain adaptation to recalcitrant substrates, in order to produce high-level fatty acid-derived chemicals from recalcitrant substrates or one carbon source of CO 2 /CH 4 , which might be applied in the future CBE production ( Pereira et al, 2019 ; Cao et al, 2020 ; Liu et al, 2020 ; Zhu et al, 2020 ). Considering oleaginous yeasts can produce high-level lipids naturally ( Wei et al, 2017b ), metabolic engineering of selected oleaginous yeasts can be an alternative choice in the future ( Kamineni and Shaw, 2020 ; Wang et al, 2020 ).…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Advances in Metabolic Engineering of Saccharomyces cerevisiae for Cocoa Butter Equivalent Production

Wang

Wei

et al. 2020

Front. Bioeng. Biotechnol.

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Cocoa butter is extracted from cocoa beans, and it is mainly used as the raw material for the production of chocolate and cosmetics. Increased demands and insufficient cocoa plants led to a shortage of cocoa butter supply, and there is therefore much interesting in finding an alternative cocoa butter supply. However, the most valuable component of cocoa butter is rarely available in other vegetable oils. Saccharomyces cerevisiae is an important industrial host for production of chemicals, enzyme and pharmaceuticals. Advances in synthetical biology and metabolic engineering had enabled high-level of triacylglycerols (TAG) production in yeast, which provided possible solutions for cocoa butter equivalents (CBEs) production. Diverse engineering strategies focused on the fatty acid-producing pathway had been applied in S. cerevisiae, and the key enzymes determining the TAG structure were considered as the main engineering targets. Recent development in phytomics and multi-omics technologies provided clues to identify potential targeted enzymes, which are responsible for CBE production. In this review, we have summarized recent progress in identification of the key plant enzymes for CBE production, and discussed recent and future metabolic engineering and synthetic biology strategies for increased CBE production in S. cerevisiae.

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Metabolic engineering for increased lipid accumulation in Yarrowia lipolytica – A Review

Cited by 159 publications

References 126 publications

Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

Whole Conversion of Agro-Industrial Wastes Rich in Galactose-Based Carbohydrates into Lipid Using Oleaginous Yeast Aureobasidium Namibiae

Advances in production of high-value lipids by oleaginous yeasts

Advances in Metabolic Engineering of Saccharomyces cerevisiae for Cocoa Butter Equivalent Production

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