Metabolic engineering of E. coli for β-alanine production using a multi-biosensor enabled approach

Yuan, Shuo‐Fu; Nair, Priya H.; Borbon, Dominic; Coleman, Sarah M.; Fan, Po-Hsun; Lin, Wen-Ling; Alper, Hal S.

doi:10.1016/j.ymben.2022.08.012

Cited by 20 publications

(2 citation statements)

References 50 publications

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“…coli − Here, we constructed a malonyl-CoA-dependent pathway for β-alanine biosynthesis, a bioprocess capable of fixing one CO 2 via enzymes AccADBCb. β-alanine can be synthesized by the transamination of malonate semialdehyde as an intermediate, which can be obtained by reducing malonyl-CoA through the C-terminal region of malonyl-CoA reductase (MCR-C) (Figure b,c) …”

Section: Resultsmentioning

confidence: 99%

Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds

Nie,

Wang,

Zhang

2023

ACS Synth. Biol.

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Acetyl-CoA is an essential central metabolite in living organisms and a key precursor for various value-added products as well. However, the intracellular availability of acetyl-CoA limits the efficient production of these target products due to complex and strict regulation. Here, we proposed a new acetyl-CoA pathway, relying on two enzymes, threonine aldolase and acetaldehyde dehydrogenase (acetylating), which can convert one L-threonine into one acetyl-CoA, one glycine, and generate one NADH, without carbon loss. Introducing the acetyl-CoA pathway could increase the intracellular concentration of acetyl-CoA by 8.6-fold compared with the wild-type strain. To develop a cost-competitive and genetically stable acetyl-CoA platform strain, the new acetyl-CoA pathway, driven by the constitutive strong promoter, was integrated into the chromosome of Escherichia coli. We demonstrated the practical application of this new acetyl-CoA pathway by high titer production of β-alanine, mevalonate, and N-acetylglucosamine. At the same time, this pathway achieved a high-yield production of glycine, a value-added commodity chemical for the synthesis of glyphosate and thiamphenicol. This work shows the potential of this new acetyl-CoA pathway for the industrial production of acetyl-CoA-derived compounds.

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Section: Resultsmentioning

confidence: 99%

Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds

Nie,

Wang,

Zhang

2023

ACS Synth. Biol.

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“…Genetically encoded biosensors that enable in vivo monitoring of cellular metabolism were created to get around this problem (Liu et al, 2015). According to Yuan et al (2022), E. coli ' s targeted evolution with a biosensor improved the production of β‐alanine. As per the studies, through biosensor‐assisted screening, E. coli mutants with increased 4HPAA production were found.…”

Section: Biosensors Application In Food Industriesmentioning

confidence: 99%

A systematic review on recent trends and perspectives of biosensors in food industries

Singh

Pandey

Srivastava

et al. 2023

Journal of Food Safety

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The food industry has inappropriate techniques for process and quality control and requires techniques that can provide information on the physical and chemical properties of food quickly and affordably. Food applications mostly focus on the identification of contaminants, with a few significant analytes, such as sugars, alcohols, amino acids, flavors, and sweeteners, as exceptions. Biosensors can bring about an analytical revolution to address the problems facing the food and agriculture industries. Potential applications for biosensors include contaminant detection, product freshness monitoring, content verification, and raw material conversion monitoring. These studies are costly and time‐consuming due to the extraction or pre‐treatment of samples. This review gives an overview of biosensors with their classification and application in different food industries such as the fruits and vegetable industry, dairy, and meat industries. The application of biosensors in detection, quality assurance, and food safety is discussed in detail with their potential application.

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Additive Manufacturing of Engineered Living Materials with Bio‐Augmented Mechanical Properties and Resistance to Degradation

Altin-Yavuzarslan

Brooks

Yuan

et al. 2023

Adv Funct Materials

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Engineered living materials (ELMs) combine living cells with polymeric matrices to yield unique materials with programmable functions. While the cellular platform and the polymer network determine the material properties and applications, there are still gaps in the ability to seamlessly integrate the biotic (cellular) and abiotic (polymer) components into singular materials, then assemble them into devices and machines. Herein, the additive-manufacturing of ELMs wherein bioproduction of metabolites from the encapsulated cells enhanced the properties of the surrounding matrix is demonstrated. First, aqueous resins are developed comprising bovine serum albumin (BSA) and poly(ethylene glycol diacrylate) (PEGDA) with engineered microbes for vat photopolymerization to create objects with a wide array of 3D form factors. The BSA-PEGDA matrix afforded hydrogels that are mechanically stiff and tough for use in load-bearing applications. Second, the continuous in situ production of l-DOPA, naringenin, and betaxanthins from the engineered cells encapsulated within the BSA-PEGDA matrix is demonstrated. These microbial metabolites bioaugmented the properties of the BSA-PEGDA matrix by enhancing the stiffness (l-DOPA) or resistance to enzymatic degradation (betaxanthin). Finally, the assembly of the 3D printed ELM components into mechanically functional bolts and gears to showcase the potential to create functional ELMs for synthetic living machines is demonstrated.

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Metabolic engineering of E. coli for β-alanine production using a multi-biosensor enabled approach

Cited by 20 publications

References 50 publications

Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds

Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds

A systematic review on recent trends and perspectives of biosensors in food industries

Additive Manufacturing of Engineered Living Materials with Bio‐Augmented Mechanical Properties and Resistance to Degradation

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