Metabolic engineering of Escherichia coli for producing adipic acid through the reverse adipate-degradation pathway

Zhao, Mei; Huang, Dixuan; Zhang, Xiaojuan; Koffas, Mattheos; Zhou, Jingwen; Deng, Yu

doi:10.1016/j.ymben.2018.04.002

Cited by 125 publications

(96 citation statements)

References 33 publications

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“…[ 94,100 ] Similar to PBS, all monomers of PBAT, such as 1,4‐BDO, adipic acid, and even TPA, can also be produced by engineered microbial strains (Table 1 and Figure 2). [ 71–80 ] In this respect, both PBAT and PBS can also be regarded as biodegradable biopolymers, which was originally thought for only PLA.…”

Section: Biodegradable Biopolymersmentioning

confidence: 99%

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Sohn

Kim

Baritugo

et al. 2020

Biotechnology Journal

116

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Advances in scientific technology in the early twentieth century have facilitated the development of synthetic plastics that are lightweight, rigid, and can be easily molded into a desirable shape without changing their material properties. Thus, plastics become ubiquitous and indispensable materials that are used in various manufacturing sectors, including clothing, automotive, medical, and electronic industries. However, strong physical durability and chemical stability of synthetic plastics, most of which are produced from fossil fuels, hinder their complete degradation when they are improperly discarded after use. In addition, accumulated plastic wastes without degradation have caused severe environmental problems, such as microplastics pollution and plastic islands. Thus, the usage and production of plastics is not free from environmental pollution or resource depletion. In order to lessen the impact of climate change and reduce plastic pollution, it is necessary to understand and address the current plastic life cycles. In this review, "sustainable biopolymers" are suggested as a promising solution to the current plastic crisis. The desired properties of sustainable biopolymers and bio-based and bio/chemical hybrid technologies for the development of sustainable biopolymers are mainly discussed.

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Section: Biodegradable Biopolymersmentioning

confidence: 99%

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Sohn

Kim

Baritugo

et al. 2020

Biotechnology Journal

116

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“…Second, CRISPR-Cas systems are versatile and can be used to transcriptionally repress or activate gene expression. CRISPRi and genome editing have been successfully applied in metabolic engineering to optimize gene expression levels for improved production of various molecules, including lycopene [ 117 ], isoprene [ 117 ], 4,4′-dihydroxybiphenyl (4HB) [ 118 ], malate [ 119 ], n-butanol [ 120 ], naringenin 7-sulfate [ 121 ], uridine [ 122 ], adipic acid [ 123 ], etc. Wu et al constructed the CRISPRi system to perturb the expression of multiple genes in the central metabolic pathway and achieved improved malonyl-CoA level by 223% [ 124 ].…”

Section: Applications Of Crispr-cas9/cas12a Biotechnology In Bacteriamentioning

confidence: 99%

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

Yao

Liu

Jia

et al. 2018

Synthetic and Systems Biotechnology

116

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CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and CRISPR-Cas12a, which have been most widely studied and used. We discuss the biological mechanisms of CRISPR-Cas as immune defense systems, recently-discovered anti-CRISPR-Cas systems, and the emerging Cas variants (such as xCas9 and Cas13) with unique characteristics. Then, we highlight various CRISPR-Cas biotechnologies, including nuclease-dependent genome editing, CRISPR gene regulation (including CRISPR interference/activation), DNA/RNA base editing, and nucleic acid detection. Last, we summarize up-to-date applications of the biotechnologies for synthetic biology and metabolic engineering in various bacterial species.

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“…By using various optimization methods, the adipate production level was gradually improved [40][41][42]46] and the highest titer reached 2.5 g/L [42]. Very recently, the adipate biosynthetic pathway from T. fusca B6 was expressed in an engineered E. coli, which could produce up to 68 g/L adipate in fed-batch fermentation [114]. The identified native pathway was basically same as the engineered reversal βoxidation pathway, and the maximal titer of adipate was 2.23 g/L from 50 g/L glucose [46].…”

Section: Reversal β-Oxidation Pathwaymentioning

confidence: 99%

“…This finding on native-occurring biosynthetic pathway is undoubtedly helpful for establishing advanced DCA overproducers. Very recently, the adipate biosynthetic pathway from T. fusca B6 was expressed in an engineered E. coli, which could produce up to 68 g/L adipate in fed-batch fermentation [114].…”

Section: Reversal β-Oxidation Pathwaymentioning

confidence: 99%

Advances in bio‐based production of dicarboxylic acids longer than C4

Qian

Zhong

2018

Engineering in Life Sciences

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Growing concerns of environmental pollution and fossil resource shortage are major driving forces for bio‐based production of chemicals traditionally from petrochemical industry. Dicarboxylic acids (DCAs) are important platform chemicals with large market and wide applications, and here the recent advances in bio‐based production of straight‐chain DCAs longer than C4 from biological approaches, especially by synthetic biology, are reviewed. A couple of pathways were recently designed and demonstrated for producing DCAs, even those ranging from C5 to C15, by employing respective starting units, extending units, and appropriate enzymes. Furthermore, in order to achieve higher production of DCAs, enormous efforts were made in engineering microbial hosts that harbored the biosynthetic pathways and in improving properties of biocatalytic elements to enhance metabolic fluxes toward target DCAs. Here we summarize and discuss the current advantages and limitations of related pathways, and also provide perspectives on synthetic pathway design and optimization for hyper‐production of DCAs.

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Metabolic engineering of Escherichia coli for producing adipic acid through the reverse adipate-degradation pathway

Cited by 125 publications

References 33 publications

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

Advances in bio‐based production of dicarboxylic acids longer than C4

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