Metabolic Engineering ofCorynebacterium glutamicumfor Cadaverine Fermentation

Mimitsuka, Takashi; Sawai, Hideki; Hatsu, Masahiro; Yamada, Katsushige

doi:10.1271/bbb.60699

Cited by 210 publications

(124 citation statements)

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“…It has been reported that recombinant strains of C. glutamicum carrying heterologous genes that encode lysine decarboxylases produce 1,5-diaminopentane. For example, Mimitsuka et al (2007) examined the effect of the introduction of the cadA gene of Escherichia coli encoding lysine decarboxylase on 1,5-diaminopentane production by a lysine-producing strain of C. glutamicum. Moreover, 1,5-diaminopentane production by a recombinant strain of C. glutamicum carrying the ldcC gene of E. coli encoding the other lysine decarboxylase, as well as the systems metabolic engineering of C. glutamicum for its enhanced production has been reported (Kind et al, 2010a(Kind et al, , 2011.…”

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confidence: 99%

Enhancement of 1,5-diaminopentane production in a recombinant strain of Corynebacterium glutamicum by Tween 40 addition

Matsushima

Hirasawa

Shimizu

2016

J. Gen. Appl. Microbiol.

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confidence: 99%

Enhancement of 1,5-diaminopentane production in a recombinant strain of Corynebacterium glutamicum by Tween 40 addition

Matsushima

Hirasawa

Shimizu

2016

J. Gen. Appl. Microbiol.

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“…While C. glutamicum has been used in industrial production of the amino acids L-glutamate and L-lysine (1,2), recent studies demonstrate its use as a platform for microbial production of other valuable compounds, including organic acids, diamines, and biofuels (3)(4)(5)(6)(7)(8)(9). Furthermore, C. glutamicum is also important as a model organism for closely related pathogenic species, such as Corynebacterium diphtheriae and Mycobacterium tuberculosis.…”

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Expanding the Regulatory Network Governed by the Extracytoplasmic Function Sigma Factor σ^Hin Corynebacterium glutamicum

et al. 2014

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bThe extracytoplasmic function sigma factor H is responsible for the heat and oxidative stress response in Corynebacterium glutamicum. Due to the hierarchical nature of the regulatory network, previous transcriptome analyses have not been able to discriminate between direct and indirect targets of H . Here, we determined the direct genome-wide targets of H using chromatin immunoprecipitation with microarray technology (ChIP-chip) for analysis of a deletion mutant of rshA, encoding an antifactor of H . Seventy-five H -dependent promoters, including 39 new ones, were identified. H -dependent, heat-inducible transcripts for several of the new targets, including ilvD encoding a labile Fe-S cluster enzyme, dihydroxy-acid dehydratase, were detected, and their 5= ends were mapped to the H -dependent promoters identified. Interestingly, functional internal H -dependent promoters were found in operon-like gene clusters involved in the pentose phosphate pathway, riboflavin biosynthesis, and Zn uptake. Accordingly, deletion of rshA resulted in hyperproduction of riboflavin and affected expression of Zn-responsive genes, possibly through intracellular Zn overload, indicating new physiological roles of H . Furthermore, sigA encoding the primary factor was identified as a new target of H . Reporter assays demonstrated that the H -dependent promoter upstream of sigA was highly heat inducible but much weaker than the known A -dependent one. Our ChIP-chip analysis also detected the H -dependent promoters upstream of rshA within the sigH-rshA operon and of sigB encoding a group 2 factor, supporting the previous findings of their H -dependent expression. Taken together, these results reveal an additional layer of the sigma factor regulatory network in C. glutamicum.

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“…Recent studies also showed the successful employment of C. glutamicum for the production of the diamines putrescine and cadaverine (5)(6)(7)(8)(9)(10), the organic acids D-lactate, succinate, 2-ketoisovalerate, and pyruvate (11)(12)(13)(14)(15), the biofuels ethanol and isobutanol (16)(17)(18), xylitol (19), and heterologous proteins (20,21).…”

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Platform Engineering of Corynebacterium glutamicum with Reduced Pyruvate Dehydrogenase Complex Activity for Improved Production of l -Lysine, l -Valine, and 2-Ketoisovalerate

Buchholz

Schwentner

Brunnenkan

et al. 2013

Appl Environ Microbiol

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Exchange of the native Corynebacterium glutamicum promoter of the aceE gene, encoding the E1p subunit of the pyruvate dehydrogenase complex (PDHC), with mutated dapA promoter variants led to a series of C. glutamicum strains with gradually reduced growth rates and PDHC activities. Upon overexpression of the L-valine biosynthetic genes ilvBNCE, all strains produced L-valine. Among these strains, C. glutamicum aceE A16 (pJC4 ilvBNCE) showed the highest biomass and product yields, and thus it was further improved by additional deletion of the pqo and ppc genes, encoding pyruvate:quinone oxidoreductase and phosphoenolpyruvate carboxylase, respectively. In fed-batch fermentations at high cell densities, C. glutamicum aceE A16 ⌬pqo ⌬ppc (pJC4 ilvBNCE) produced up to 738 mM (i.e., 86.5 g/liter) L-valine with an overall yield (Y P/S ) of 0.36 mol per mol of glucose and a volumetric productivity (Q P ) of 13.6 mM per h [1.6 g/(liter ؋ h)]. Additional inactivation of the transaminase B gene (ilvE) and overexpression of ilvBNCD instead of ilvBNCE transformed the L-valine-producing strain into a 2-ketoisovalerate producer, excreting up to 303 mM (35 g/liter) 2-ketoisovalerate with a Y P/S of 0.24 mol per mol of glucose and a Q P of 6.9 mM per h [0.8 g/(liter ؋ h)]. The replacement of the aceE promoter by the dapA-A16 promoter in the two C. glutamicum L-lysine producers DM1800 and DM1933 improved the production by 100% and 44%, respectively. These results demonstrate that C. glutamicum strains with reduced PDHC activity are an excellent platform for the production of pyruvate-derived products.

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Metabolic Engineering ofCorynebacterium glutamicumfor Cadaverine Fermentation

Cited by 210 publications

References 18 publications

Enhancement of 1,5-diaminopentane production in a recombinant strain of <i>Corynebacterium glutamicum</i> by Tween 40 addition

Enhancement of 1,5-diaminopentane production in a recombinant strain of <i>Corynebacterium glutamicum</i> by Tween 40 addition

Expanding the Regulatory Network Governed by the Extracytoplasmic Function Sigma Factor σ^Hin Corynebacterium glutamicum

Platform Engineering of Corynebacterium glutamicum with Reduced Pyruvate Dehydrogenase Complex Activity for Improved Production of l -Lysine, l -Valine, and 2-Ketoisovalerate

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Metabolic Engineering ofCorynebacterium glutamicumfor Cadaverine Fermentation

Cited by 210 publications

References 18 publications

Enhancement of 1,5-diaminopentane production in a recombinant strain of <i>Corynebacterium glutamicum</i> by Tween 40 addition

Enhancement of 1,5-diaminopentane production in a recombinant strain of <i>Corynebacterium glutamicum</i> by Tween 40 addition

Expanding the Regulatory Network Governed by the Extracytoplasmic Function Sigma Factor σHin Corynebacterium glutamicum

Platform Engineering of Corynebacterium glutamicum with Reduced Pyruvate Dehydrogenase Complex Activity for Improved Production of l -Lysine, l -Valine, and 2-Ketoisovalerate

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Expanding the Regulatory Network Governed by the Extracytoplasmic Function Sigma Factor σ^Hin Corynebacterium glutamicum