2015
DOI: 10.1021/acs.jafc.5b04279
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Metabolic Engineering of Wheat Provitamin A by Simultaneously Overexpressing CrtB and Silencing Carotenoid Hydroxylase (TaHYD)

Abstract: Increasing the provitamin A content in staple crops via carotenoid metabolic engineering is one way to address vitamin A deficiency. In this work a combination of methods was applied to specifically increase β-carotene content in wheat by metabolic engineering. Endosperm-specific silencing of the carotenoid hydroxylase gene (TaHYD) increased β-carotene content 10.5-fold to 1.76 μg g(-1) in wheat endosperm. Overexpression of CrtB introduced an additional flux into wheat, accompanied by a β-carotene increase of … Show more

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Cited by 63 publications
(38 citation statements)
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“…10-fold enrichment [293,294]. Overexpression of crtB together with endospermspecific silencing of an endogenous BCH gene (TaHYD) was also successful to increase β-carotene content to 2.45 μg/g DW in wheat endosperm [295].…”
Section: Other Cerealsmentioning
confidence: 95%
“…10-fold enrichment [293,294]. Overexpression of crtB together with endospermspecific silencing of an endogenous BCH gene (TaHYD) was also successful to increase β-carotene content to 2.45 μg/g DW in wheat endosperm [295].…”
Section: Other Cerealsmentioning
confidence: 95%
“…By simultaneously introducing the PaCRTB and the PaCRTI genes into a low carotenoid bread wheat varieties, the same authors achieved 8-fold increase in the total carotenoid content up to 4.76 µg/g and 76-fold increase in the provitamin A carotenoid content that reached 3.82 µg/g, most of which was represented by β-carotene (3.21 µg/g) [38] (Table 1). Further increase in the β-carotene content was obtained by the same research group by combining the endosperm-specific silencing of the HYD gene and the overexpression of the bacterial CRTB gene [39]. Through this combination of methods, significant levels of β-carotene accumulation were obtained, corresponding to an increase of up to 31-fold and a concentration of up to 5.04 µg/g (Table 1).…”
Section: Compositionmentioning
confidence: 95%
“…To achieve a high level of β-carotene accumulation, suppressing the activity of LYCE by silencing StLCY-e in potato or the activity of HYDB by silencing StCHY-𝜀 in potato is adopted to ensure the appropriate direction of the metabolic flux (Diretto et al, 2007; Van Eck et al, 2007). In wheat, simultaneous overexpression of CrtB and silencing of HYDB achieves an up to 31-fold increase of β-carotene up to 5.06 μg g -1 (Zeng et al, 2015). Another approach is to make use of the Orange ( Or ) gene from cauliflower ( Brassica oleracea var.…”
Section: Manipulation Of Vitamin Metabolismmentioning
confidence: 99%