Metabolic enzyme activities in black bream (Acanthopagrus butcheri) from the Swan-Canning Estuary, Western Australia

Webb, Diane; Gagnon, Marthe Monique; Rose, Tom

doi:10.1016/j.cbpc.2005.07.010

Cited by 7 publications

(6 citation statements)

References 21 publications

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“…CCO activity was similar in fish collected from Claisebrook Cove, relative to the reference site, Burswood Lake. Webb et al (2005) noted a reduction in CCO activity in the gills of black bream collected at a site contaminated by PAHs. As CCO activity correlates well with the actual oxygen consumption rates in various tissues, it appears that the Claisebrook Cove black bream aerobic metabolism in the gills was not affected by exposure to bioavailable contaminants.…”

Section: Discussionmentioning

confidence: 99%

Biomarker responses and PAH ratios in fish inhabiting an estuarine urban waterway

Duarte

Sadauskas-Henrique

Almeida-Val

et al. 2017

Environmental Toxicology

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Many cities worldwide are established adjacent to estuaries and their catchments resulting in estuarine contamination due to intense anthropogenic activities. The aim of this study was to evaluate if fish living in an estuarine urban waterway were affected by contamination, via the measurement of a suite of biomarkers of fish health. Black bream (Acanthopagrus butcheri) were sampled in a small urban embayment and a suite of biomarkers of fish health measured. These were condition factor (CF), liver somatic index (LSI), gonadosomatic index (GSI), hepatic EROD activity, polycyclic aromatic hydrocarbon (PAH) biliary metabolites, serum sorbitol dehydrogenase (s-SDH) and branchial enzymes cytochrome C oxidase (CCO), and lactate dehydrogenase (LDH) activities. The biomarkers of exposure EROD activity, and pyrene- and B(a)P-type biliary metabolites confirmed current or recent exposure of the fish and that fish were metabolizing contaminants. Relative to a reference site, LSI was higher in fish collected in the urban inlet as was the metabolic enzyme LDH activity. CF, GSI, s-SDH, CCO, and naphthalene-type metabolites were at similar levels in the urban inlet relative to the reference site. PAH biliary metabolite ratios of high-molecular-weight to low-molecular-weight suggest that fish from the urban inlet were exposed to pyrogenic PAHs, likely from legacy contamination and road runoff entering the embayment. Similarly, the sediment PAH ratios and the freshness indices suggested legacy contamination of a pyrogenic source, likely originating from the adjacent historic gasworks site and a degree of contamination of petrogenic nature entering the inlet via storm water discharge. Biomarkers of exposure and effect confirmed that black bream collected in the Claisebrook Cove inlet, Western Australia, are currently exposed to contamination and are experiencing metabolic perturbations not observed in fish collected at a nearby reference site.

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Section: Discussionmentioning

confidence: 99%

Biomarker responses and PAH ratios in fish inhabiting an estuarine urban waterway

Duarte

Sadauskas-Henrique

Almeida-Val

et al. 2017

Environmental Toxicology

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“…Metals bioaccumulated in fish organs bind to or interact with cytosolic molecules and can interfere with the cell functions, leading to various deleterious effects [6][7] . On the other hand, metals also play an important physiological role in the living organisms, since many biological functions critically depend on the interaction with certain metals within the cell; for example, several proteins require a metal ion to be able to perform their catalytic activities, to stabilize their structures and to properly perform their functions [8][9][10] . Therefore, metal physiological functions, as well as their possible toxic effects on metabolism and homeostasis of fish organisms can be assessed by analyses of various metal binding biomolecules within the cells, such as various metalloenzymes and other metalloproteins [11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Characterization and identification of selected metal-binding biomolecules from hepatic and gill cytosols of Vardar chub (Squalius vardarensis Karaman, 1928) using various techniques of liquid chromatography and mass spectrometry

et al. 2019

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“…White muscle LDH activity determined in this study in the control groups (1,475.2 ± 129.8 U/mg protein in the control and acetone control, respectively) is lower than that reported by Antognelli et al [29] for the same species. However, they are in the range reported for the black bream, Acanthopagrus butcheri (between 700 and 4,000 U/mg protein) [30], and higher than the corresponding values determined in the Australian sea bass, Macquaria novemaculeata (∼600 U/mg protein) [31].…”

Section: Discussionmentioning

confidence: 57%

Toxicity ranking of estuarine sediments on the basis of Sparus aurata biomarkers

Cunha

Neuparth

Caeiro

et al. 2007

Enviro Toxic and Chemistry

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Sparus aurata biomarkers were used to rank sediments from the Sado River estuary (Portugal) according to their toxicity. Initially, the activities of liver ethoxyresorufin-O-deethylase, liver and gill glutathione S-transferases, muscle lactate dehydrogenase, and brain acetylcholinesterase were tested in a laboratory bioassay with the reference compound benzo[a]pyrene. Enzymatic activities were determined in different tissues of fish exposed for 48, 96, or 240 h to three concentrations of benzo[a]pyrene (25, 50, and 100 microg/L). Induction of liver ethoxyresorufin-O-deethylase was observed at all the exposure periods and concentrations, suggesting a continuous response of this system to toxicant exposure. Induction of liver glutathione S-transferases activity was only observed after 240 h of exposure, whereas gill glutathione S-transferases activity was significantly inhibited at all the exposure periods, suggesting a direct or indirect effect of the toxicant on these enzymes. Inhibition of lactate dehydrogenases activity was only observed after 96 h of exposure to 25 microg/L of benzo[a]pyrene. No significant effects were observed on acetylcholinesterase activity, suggesting that cholinergic function of S. aurata is not affected by benzo[a]pyrene. In a second phase, fish were exposed for 240 h to sediments collected at five sites of the Sado River estuary, and the same biomarkers were analyzed. For all the enzymes assayed, significant differences among sites were found. In this study, the battery of biomarkers used allowed to discrimination among sites with different types of contamination, levels of contamination, or both, after multivariate data analysis. Discrimination of sites was similar to the ranking provided by a more complex and parallel study (including chemical analysis of sediments, macrobenthic community analysis, amphipod mortality toxicity tests, and sea urchin abnormality embryo assays), suggesting its suitability to evaluate the toxicity of estuarine sediments.

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Metabolic enzyme activities in black bream (Acanthopagrus butcheri) from the Swan-Canning Estuary, Western Australia

Cited by 7 publications

References 21 publications

Biomarker responses and PAH ratios in fish inhabiting an estuarine urban waterway

Biomarker responses and PAH ratios in fish inhabiting an estuarine urban waterway

Characterization and identification of selected metal-binding biomolecules from hepatic and gill cytosols of Vardar chub (Squalius vardarensis Karaman, 1928) using various techniques of liquid chromatography and mass spectrometry

Toxicity ranking of estuarine sediments on the basis of Sparus aurata biomarkers

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