Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique

Choi, Hyung‐Kyoon; Choi, Young Hae; Verberne, Marianne C.; Lefeber, Alfons W.M.; Erkelens, C.; Verpoorte, Robert

doi:10.1016/j.phytochem.2004.01.019

Cited by 181 publications

(143 citation statements)

References 23 publications

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“…Consequently, as the derivative of choline, phosphocholine was decreased consistently with the decreased choline. The decrease of glucose in salinity-treated plants after 1 week exposure indicated the rapid utilization of this compound for the production of other compounds such as citrate, malate, and fumarate, or a lower production of carbohydrates (Choi et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Liu

You

et al. 2011

Plant Mol Biol Rep

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Suaeda salsa is a native halophyte in saline soils. Salinity is the most important environmental constraint for plant productivity in the Yellow River Delta. In this work, we investigated the salt-induced effects in root of S. salsa exposed to two environmentally relevant salinities for 1 week and 1 month using nuclear magnetic resonance-based metabolomics. Our results indicated that salt stress inhibited the growth of S. salsa and induced significant metabolic responses including decreased amino acids, lactate, 4-aminobutyrate, malate, choline, phosphocholine, and increased betaine, sucrose, and allantoin in root tissues of S. salsa. In addition, salinity exposures upregulated the activities of superoxide dismutase, glutathione S-transferases, peroxidase, catalase, and glutathione peroxidase in the aboveground part of seedlings of S. salsa after exposures. Overall, these results demonstrated the osmotic and oxidative stresses, disturbances in protein biosynthesis/degradation, and energy metabolism in S. salsa exposed to salinities.

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Section: Discussionmentioning

confidence: 99%

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Liu

You

et al. 2011

Plant Mol Biol Rep

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“…In fact, NMR-based metabolomics has been widely used to decode the broad range of chemical compounds that might be implicated in the plant defence against microbe attacks (Choi et al, 2006;Ward et al, 2010). Our group has been applying this technology to the detection and structure elucidation of metabolites in plants upon 105 treatment with a wide range of biotic and abiotic stress agents (Choi et al, 2004;Simoh et al, 2009;Jahangir et al, 2009;Mirnezhad et al, 2010;Simoh et al, 2010). In recent years, very detailed NMR metabolomic studies on both compatible and incompatible plant-pathogen interactions have been reported (Lima et al, 2010;Bollina et al, 2010;Ward et al, 2010;Jones et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

López-Gresa

Lisón

Kim

et al. 2012

Journal of Plant Physiology

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general time-dependent decrease in organic acids, amino acids (excluding asparagine), phenylpropanoids and rutin was observed in individual leaves. In addition, metabolite alterations were also found to correlate with the developmental stage of the leaf: high levels of organic acids, some amino acids, phenylpropanoids, and flavonoids were found in lower leaves while elevated amounts of sugars were present in the upper ones. 35Moreover, a marked variation in the content of some metabolites was also observed to be associated to the asymptomatic ToMV infection both in inoculated and systemically infected leaves. While flavonoids accumulated in virus-inoculated leaves, increased levels of phenylpropanoids were observed in non-inoculated leaves where ToMV actively replicates. Finally, diurnal changes in the metabolite content were also 40 observed: an increase of amino acids and organic acids (except glutamic acid) were observed in the samples collected in the morning, whereas sugars and secondary metabolite levels increased in the tomato leaves harvested in the evening.

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“…From a practical point of view, monitoring of unexpected effects of metabolic engineering is clearly of great importance and metabolic profiling analyses that cover the widest range of metabolites may prove particularly informative. For this purpose, non-targeted methods based on GC-MS (Fiehn et al 2000;Garrat et al 2005;Roessner-Tunali et al 2003), HPLC-UV (photodiode array detection) (Fraser et al2000), HPLC-MS (Huhman and Sumner 2002;Roepenack-Lahaye et al 2004), 1 H-NMR (Baker et al 2006;Choi et al 2004;Gall et al 2003;Manetti et al 2004; see also the review by Ward et al 2007), and high-resolution MS (Hirai et al 2004;Oikawa et al 2006) have been applied. Given the wide chemical and quantitative diversity of plant metabolites, however, no single analytical methodology can provide a definitive and absolute metabolite profile.…”

Section: Methodology Of Metabolite Profiling Of Plantsmentioning

confidence: 99%

“…Among the various multi-variate analysis techniques, principal component analysis (PCA) appears to be used most frequently in order to evaluate metabolic changes in GM crops (Choi et al 2004;Fiehn et al 2000;Gall et al 2003). Evaluation of loading plots can be used to find those metabolites most affected by genetic modification and which most contribute to the genotype difference (Baker et al 2006;Defernez et al 2004;Taylor et al 2002).…”

Section: Methodology Of Metabolite Profiling Of Plantsmentioning

confidence: 99%

Metabolomics for metabolically manipulated plants: effects of tryptophan overproduction

et al. 2007

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Advances in molecular breeding technologies have enabled manipulation of the concentrations of specific plant components by modifying the genes that play a key role in their production. This has provided new opportunities to enhance the nutritional quality of major crops. However, given that metabolic pathways form a highly integrated network, any alteration in a given biosynthetic pathway is most likely to effect secondary and unpredicted changes in the metabolite profile of other pathways. Metabolomics technologies can contribute to the efficient detection of such unexpected effects caused by genetic modification. This has relevance not only from the perspective of safety evaluations of newly developed crops, but to basic science focused on uncovering hitherto unknown regulatory mechanisms associated with the biosynthesis and catabolism of primary and secondary metabolites in plants. In this review, recent advances in plant metabolic engineering for the overproduction of tryptophan (Trp), one of the essential amino acids, are described. In particular, the efficacy of a transgene OASA1D that encodes a mutant anthranilate synthase (AS) a subunit of rice in specifically elevating levels of Trp without marked secondary effects on the metabolite profile of rice is demonstrated. Related topics, such as regulation of Trp biosynthesis, possible interactions between the biosyntheses of Trp and other aromatic amino acids, and translocation of Trp in are discussed based on findings derived from metabolomic analyses of Trp-overproducing transgenic plants.

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Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique

Cited by 181 publications

References 23 publications

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Metabolic fingerprinting of Tomato Mosaic Virus infected Solanum lycopersicum

Metabolomics for metabolically manipulated plants: effects of tryptophan overproduction

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