Metabolic flux change in Klebsiella pneumoniae L17 by anaerobic respiration in microbial fuel cell

Kim, Changman; Ainala, Satish Kumar; Oh, You-Kwan; Jeon, Byong‐Hun; Park, Sunghoon; Kim, Eun Jung

doi:10.1007/s12257-015-0777-6

Cited by 20 publications

(26 citation statements)

References 41 publications

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“…Figures (d) and 3(f) show the carbon paper surface, emphasizing the bright regions where the Ti nanoparticles are attached. According to our results, most of the Ti is present on the surface when deposited with an e‐beam evaporator, and more Ti is attached to the electrode dipped for a longer time (48 h) …”

Section: Resultsmentioning

confidence: 60%

“…Either 20 mmol L −1 dl ‐lactic acid or 5 g L −1 glucose was used as carbon source for MR‐1 and L17 respectively. Trace mineral and vitamin solutions were added at the same concentration as that reported in a previous study . The mineral solution stocks were used in the following composition (L −1 DW): 2.14 g nitrilotriacetic acid, 0.1 g MnCl 2 ·4H 2 O, 0.3 g FeSO 4 ·7H 2 O, 0.17 g CoCl 2 ·6H 2 O, 0.2 g ZnSO 4 ·7H 2 O, 0.3 g CuCl 2 ·2H 2 O, 0.005 g H 3 BO 3 , 0.09 g Na 2 MoO 4 , 0.11 g NiSO 4 ·6H 2 O, 0.2 g Na 2 WO 4 ·2H 2 O.…”

Section: Methodsmentioning

confidence: 99%

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Enhancement of bioelectricity generation by a microbial fuel cell using Ti nanoparticle‐modified carbon electrode

Kim

Heo

2019

J of Chemical Tech & Biotech

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BACKGROUND Microbial fuel cells (MFCs) are promising devices that can be used to generate electricity from organic wastewater through microbial redox reactions. Various strategies have been attempted to improve the power generation of MFCs, including electrode modification. Titanium (Ti) is a biocompatible metal which is commonly used in various applications. This study examined the improvement of voltage generation by Ti nanoparticle attachment to the carbon electrode surface of an MFC by simple dipping and e‐beam evaporation. Two microbes, namely Shewanella oneidensis MR‐1 and Klebsiella pneumoniae L17, having different electron transfer mechanisms were used to identify the effects of Ti nanoparticles on bioelectricity generation. RESULTS Voltage generation was significantly increased for the MFCs containing Ti nanoparticles, both using S. oneidensis MR‐1 and K. pneumoniae L17. Higher concentrations of DNA extracted from the electrode surface indicated that the Ti nanoparticles did not only assist the electron transfer process from the bacteria to the electrode but also microbial attachment on the carbon electrode. Energy‐dispersive X‐ray spectroscopy (EDS) experiments demonstrated the sustainability of the Ti nanoparticles, showing no significant changes in the attached Ti nanoparticles during an operation period of 3 weeks. CONCLUSION It was demonstrated that the Ti‐dipping method is applicable to MFCs as an electrode modification strategy by Ti nanoparticle formation, leading to a similar level of voltage generation (but through a much simpler process) as compared with conventional evaporation methods. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Enhancement of bioelectricity generation by a microbial fuel cell using Ti nanoparticle‐modified carbon electrode

Kim

Heo

2019

J of Chemical Tech & Biotech

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“…Maximization of the cell growth or 1,3‐PDO production pathways were the objective functions for the simulation in BESs and non‐BESs. Linear optimization using the MetaFluxNet program was implemented to solve the metabolic flux equations …”

Section: Methodsmentioning

confidence: 99%

“…The interaction between microbes and a carbon electrode has been reported to alter the microbial intracellular redox balance, shifting the conversion process towards a more reduced or oxidized state . Previous studies have shown that electrode‐based regulation triggered a change in the metabolic (carbon) fluxes, and concurrently produced more reduced/oxidized metabolites, such as 3‐hydroxypropionic acid, l ‐lysine, and acetoin . EF provides a potential platform to further improve the titer and yield, particularly for targeted oxidized/reduced products in biochemical reactions, which are difficult to achieve through conventional fermentation …”

Section: Introductionmentioning

confidence: 99%

“…Among the K. pneumoniae strains, K. pneumoniae L17 has the ability to exchange respiratory electrons with the electrode . Previous studies have reported that the application of an electrical potential through a carbon electrode in a bioelectrochemical system (BES) induced a change in the metabolic flux and gene expression of L17 . Such anodic EF increased the productivity of 3‐HP 1.8 fold using a +0.5 V (versus Ag/AgCl) polarized electrode in a BES …”

Section: Introductionmentioning

confidence: 99%

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Small Current but Highly Productive Synthesis of 1,3‐Propanediol from Glycerol by an Electrode‐Driven Metabolic Shift in Klebsiella pneumoniae L17

et al. 2020

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Electrofermentation actively regulates the bacterial redox state, which is essential for bioconversion and has been highlighted as an effective method for further improvements of the productivity of either reduced or oxidized platform chemicals. 1,3‐Propanediol (1,3‐PDO) is an industrial value‐added chemical that can be produced from glycerol fermentation. The bioconversion of 1,3‐PDO from glycerol requires additional reducing energy under anoxic conditions. The cathode‐based conversion of glycerol to 1,3‐PDO with various electron shuttles (2‐hydroxy‐1,4‐naphthoquinone, neutral red, and hydroquinone) using Klebsiella pneumoniae L17 was investigated. The externally poised potential of −0.9 V vs. Ag/AgCl to the cathode increased 1,3‐PDO (35.5±3.1 mm) production if 100 μm neutral red was used compared with non‐bioelectrochemical system fermentation (23.7±2.4 mm). Stoichiometric metabolic flux and transcriptional analysis indicated a shift in the carbon flux toward the glycerol reductive pathway. The homologous overexpression of glycerol dehydratase (DhaB) and 1,3‐PDO oxidoreductase (DhaT) enzymes synergistically enhanced 1,3‐PDO conversion (39.3±0.8 mm) under cathode‐driven fermentation. Interestingly, a small current uptake (0.23 mmol of electrons) caused significant metabolic flux changes with a concomitant increase in 1,3‐PDO production. This suggests that both an increase in 1,3‐PDO production and regulation of the cellular metabolic pathway are feasible by electrode‐driven control in cathodic electrofermentation.

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Measurement of crude-cell-extract glycerol dehydratase activity in recombinantEscherichia coliusing coupled-enzyme reactions

Sankaranarayanan

Seol

Kim

et al. 2017

Journal of Industrial Microbiology and Biotechnology

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Glycerol dehydratase (GDHt), which converts glycerol to 3-hydroxypropionaldehyde, is essential to the production of 1,3-propanediol (1,3-PDO) or 3-hydroxypropionic acid (3-HP). A reliable GDHt activity assay in crude-cell extract was developed. In the assay, GDHt converted 1,2-propanediol (1,2-PDO) to propionaldehyde, which was further converted to 1-propionic acid by aldehyde dehydrogenase (KGSADH) or to 1-propanol by yeast-alcohol dehydrogenase (yADH), while the NADH concentration change was monitored spectrophotometrically. Cells should be disintegrated by Bead Beater/French Press, not by chemical methods (BugBuster/B-PER™), because the reagents significantly inactivated GDHt and coupling enzymes. Furthermore, in the assay mixture, a much higher activity of KGSADH (>200-fold) or yADH (>400-fold) than that of GDHt should have been maintained. Under optimal conditions, both KGSADH and yADH showed practically the same activity. The coupled-enzyme assay method established here should prove to be applicable to recombinant strains developed for the production of 3-HP and/or 1,3-PDO from glycerol.

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Metabolic flux change in Klebsiella pneumoniae L17 by anaerobic respiration in microbial fuel cell

Cited by 20 publications

References 41 publications

Enhancement of bioelectricity generation by a microbial fuel cell using Ti nanoparticle‐modified carbon electrode

Enhancement of bioelectricity generation by a microbial fuel cell using Ti nanoparticle‐modified carbon electrode

Small Current but Highly Productive Synthesis of 1,3‐Propanediol from Glycerol by an Electrode‐Driven Metabolic Shift in Klebsiella pneumoniae L17

Measurement of crude-cell-extract glycerol dehydratase activity in recombinantEscherichia coliusing coupled-enzyme reactions

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