Metabolic heat production as a measure of macrophage response to particles from orthopedic implant materials

Charlebois, S.; Daniels, A. U.; Smith, Richard A.

doi:10.1002/jbm.1230

Cited by 18 publications

(12 citation statements)

References 31 publications

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“…We have examined IC-21 macrophages after particle exposure in a related study 17 and showed by confocal microscopy that phagocytosis does indeed occur with these particles and this macrophage cell line. Also, the various changes in macrophage secretory responses suggest that binding of LPS to both HDPE and Co alloy particles (as well as to planar surfaces of polypropylene) occurred.…”

Section: Discussionmentioning

confidence: 98%

Macrophage cytokine response to particles and lipopolysaccharidein vitro

Daniels

Barnes

Charlebois

et al. 2000

J. Biomed. Mater. Res.

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Several investigators have suggested that biologic molecules adsorbed onto particles may play a key role in determining macrophage response. Adsorbed endotoxins (bacterial debris) may be of particular importance since they are widely present exogenously and endogenously and adhere strongly to many materials. Murine-transformed peritoneal macrophages (IC-21) were used in this in vitro study. Secretions of IL-1 beta, TNF alpha, and IL-6 were used as a measure of macrophage response to micron-range particles of high-density polyethylene and Co-Cr-Mo alloy, with and without adsorbed lipopolysaccharide (LPS) endotoxin. Little cytokine secretion was measured in response to particles (and to polypropylene experimental chambers) cleaned with ethanol and saline and not exposed to LPS. The lack of macrophage response to cleaned particles has been reported by others and may help reconcile conflicting reports in the literature. Cytokine secretion levels were high in all cases if the chambers (with or without particles) were exposed to LPS (and rinsed to minimize nonbound LPS). Secretion patterns were different with particles present and for polymer versus metal particles. Overall, these results suggest that (1) adsorbed molecules on material surfaces strongly affect macrophage response and (2) particle surface chemistry and microstructure affect the concentration and configuration of adsorbed molecules, further influencing particle interaction with macrophage surface receptors.

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Section: Discussionmentioning

confidence: 98%

Macrophage cytokine response to particles and lipopolysaccharidein vitro

Daniels

Barnes

Charlebois

et al. 2000

J. Biomed. Mater. Res.

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“…These studies have examined activation of signal transduction pathways, 40 -42 metabolic activity, 43 and production of proinflammatory cytokines 41,[43][44][45][46][47] and have examined virtually all orthopedically relevant types of particles, including titanium, 42,44,47 cobaltchrome, [43][44][45] PMMA, 40,42,46 polyethylene, 43,45 and hydroxyapatite. 42 Most importantly, Akisue et al 41 have further extended these studies to include authentic titanium alloy particles retrieved from patients with aseptic loosening.…”

Section: Endotoxin Increases Biological Activity Of Wear Particles Inmentioning

confidence: 99%

Does endotoxin contribute to aseptic loosening of orthopedic implants?

et al. 2004

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Aseptic loosening of orthopedic implants caused by wear particles is a major clinical problem. This review examines the hypothesis that bacterial endotoxin contributes to aseptic loosening. Clinical findings support this hypothesis: bacterial biofilms exist on many implants from patients with aseptic loosening and antibiotics in bone cement reduce the rate of aseptic loosening. Three approaches were used to demonstrate that adherent endotoxin increases bioactivity of titanium particles. These experiments measured cytokine production and osteoclast differentiation in vitro and murine calvarial osteolysis in vivo. First, removal of >99.9% of the adherent endotoxin from titanium particles significantly ablates their biological activity. Second, adding lipopolysaccharide back to these "endotoxin-free" particles restores their biological activity. Third, cells or mice that are genetically hyporesponsive to endotoxin are significantly less responsive to titanium particles than are wild-type controls. Other investigators have confirmed and extended these results to include virtually all orthopedically relevant types of particles, including authentic titanium alloy particles retrieved from patients with loosening. Our recent studies suggest that adherent endotoxin on orthopedic implants may also inhibit initial osseointegration of the implants. Taken together, these studies suggest that bacterial endotoxin may have a significant role in induction of aseptic loosening.

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“…The heat source production value used in this study resulted in temperature differences that approximate the values that have been reported in literature most recently. This value, 5 mW mm À3 , is higher than macrophage heat production values that have been reported in literature (Thore´n et al, 1990;Charlebois et al, 2002). In addition, the catheter geometry was simplified, whereas in reality it can be more complex.…”

Section: Discussionmentioning

confidence: 60%