Metabolic Labeling of Peptidoglycan with NIR‐II Dye Enables In Vivo Imaging of Gut Microbiota

Wang, Wei; Yang, Qinglai; Du, Yahui; Zhou, Xiaobo; Du, Xiaochen; Wu, Qiuyue; Lin, Liang; Song, Yanling; Li, Fuyou; Yang, Chaoyong; Tan, Weihong

doi:10.1002/anie.201910555

Cited by 89 publications

(60 citation statements)

References 43 publications

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“…), where the quantity of bacteria is relatively low and the distributions are often random and irregular [2a, 10] . We recently reported a near infrared II (NIR‐II)‐based macrographic imaging method to visualize the distribution of gut microbiota in vivo [11] . However, owing to the diffraction limit of NIR‐II fluorescence, [12] this method cannot provide satisfactory spatial resolutions to precisely localize bacteria tissue‐wide.…”

Section: Introductionmentioning

confidence: 99%

Three‐Dimensional Quantitative Imaging of Native Microbiota Distribution in the Gut

Wang

Zhang

et al. 2020

Angew Chem Int Ed

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Owing to the challenges to acquire detailed spatial information of gut bacteria in situ, three‐dimensional (3D) microbiota distributions in the gut remain largely uncharted. Here, we propose a tissue clearing‐based and D‐amino acid labeling‐facilitated (TiDaL) strategy that combines a novel microbiota in vivo labeling protocol, CUBIC‐based tissue clearing and whole‐mount tissue imaging, to achieve 3D imaging of indigenous gut microbiota. We demonstrate high‐resolution 3D acquisition of their biogeography in different gut sections, and present quantitative spatial details in relation to the host epithelium. We unexpectedly observe microbiota in the small intestine crypts, which were thought to be bacteria‐free. Significant bacterial overgrowth in the first two‐thirds of the small intestine is detected in an enteritis model. We expect that this quantitative 3D imaging strategy for native gut microbiota will provide insightful information into the host–microbiota interactions.

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Section: Introductionmentioning

confidence: 99%

Three‐Dimensional Quantitative Imaging of Native Microbiota Distribution in the Gut

Wang

Zhang

et al. 2020

Angew Chem Int Ed

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“…Significant labeling was observed with millimolar concentrations of d ‐amino acids (Figure 2B); this is comparable with previous publications, in which a concentration of 0.1–10 m m was often used. [ 24–28 ] The fluorescence intensity versus incubation time plateaued at 30 min, which is close to the doubling time (generation time) of S. aureus (Figure S1, Supporting Information). It was also found that 10 min sufficed to complete the reaction when 20 μ m of Cy5‐Tz was used (Figure S2, Supporting Information).…”

Section: Resultsmentioning

confidence: 58%

“…[ 21–23 ] Imaging bacteria with d ‐amino acids in complex biological environment has demonstrated excellent bacteria targeting performance. [ 24–28 ]…”

Section: Introductionmentioning

confidence: 99%

Metabolic Labeling Mediated Targeting and Thermal Killing of Gram‐Positive Bacteria by Self‐Reporting Janus Magnetic Nanoparticles

Hou

Mahadevegowda

et al. 2020

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Nanoparticles have been widely used in detection and killing of bacteria; however, targeting bacteria is still challenging. Delicate design of nanoparticles is required for simultaneous targeting, detection, and therapeutic functions. Here the use of Au/MnFe2O4 (Au/MFO) Janus nanoparticles to target Gram‐positive bacteria via metabolic labeling is reported and realize integrated self‐reporting and thermal killing of bacteria. In these nanoparticles, the Au component is functionalized with tetrazine to target trans‐cyclooctene group anchored on bacterial cell wall by metabolic incorporation of d‐amino acids, and the MFO part exhibits peroxidase activity, enabling self‐reporting of bacteria before treatment. The spatial separation of targeting and reporting functions avoids the deterioration of catalytic activity after surface modification. Also important is that MFO facilitates magnetic separation and magnetic heating, leading to easy enrichment and magnetic thermal therapy of labeled bacteria. This method demonstrates that metabolic labeling with d‐amino acids is a promising strategy to specifically target and kill Gram‐positive bacteria.

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“…recognition and transduction is more utilized due to its high flexibility in designing and engineering for different application scenarios. [306,307]. Until now, the fluorescent reporting strategies still play major roles in nucleic acidbased bioanalysis, especially in the biological and biomedical applications.…”

Section: Signal Recognition and Transduction Strategiesmentioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

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In recent years, multi-modal entity linking (MEL) has garnered increasing attention in the research community due to its significance in numerous multi-modal applications. Video, as a popular means of information transmission, has become prevalent in people's daily lives. However, most existing MEL methods primarily focus on linking textual and visual mentions or offline videos's mentions to entities in multi-modal knowledge bases, with limited efforts devoted to linking mentions within online video content. In this paper, we propose a task called Online Video Entity Linking (OVEL), aiming to establish connections between mentions in online videos and a knowledge base with high accuracy and timeliness. To facilitate the research works of OVEL, we specifically concentrate on live delivery scenarios and construct a live delivery entity linking dataset called LIVE. Besides, we propose an evaluation metric that considers timelessness, robustness, and accuracy. Furthermore, to effectively handle OVEL task, we leverage a memory block managed by a Large Language Model and retrieve entity candidates from the knowledge base to augment LLM performance on memory management. The experimental results prove the effectiveness and efficiency of our method.

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Metabolic Labeling of Peptidoglycan with NIR‐II Dye Enables In Vivo Imaging of Gut Microbiota

Cited by 89 publications

References 43 publications

Three‐Dimensional Quantitative Imaging of Native Microbiota Distribution in the Gut

Three‐Dimensional Quantitative Imaging of Native Microbiota Distribution in the Gut

Metabolic Labeling Mediated Targeting and Thermal Killing of Gram‐Positive Bacteria by Self‐Reporting Janus Magnetic Nanoparticles

Nucleic Acids Analysis

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