Metabolic pathway of magnetized fluid‐induced relaxation effects on heart muscle

Ayrapetyan, Gayane; Papanyan, Alexander; Hayrapetyan, Hovik; Ayrapetyan, Sinerik

doi:10.1002/bem.20145

Cited by 20 publications

(19 citation statements)

References 17 publications

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“…metabolism has a crucial role (Adey 1993;Blackman et al 1985Blackman et al , 1988. It was also shown that SMF had elevation effect on intracellular cyclic guanosine monophosphate (cGMP) (Ayrapetyan et al 1994(Ayrapetyan et al , 2005, which leads to the activation of Na ? /Ca 2?…”

Section: Introductionmentioning

confidence: 97%

Age dependency of high-affinity ouabain receptors and their magnetosensitivity

et al. 2012

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Age dependency of [ 3 H]-ouabain binding, 45 Ca 2? eflux and its magnetosensitivity in rats' brain cortex and heart muscle tissues were studied. Curves of dose-dependent [ 3 H]-ouabain binding consisted of three components with different affinities (10 -7 -10 -4 M (a 1 ); 10 -9 -10 -7 M (a 2 ); and 10 -11 -10 -9 M (a 3 )). These curves were also characterized by different dose-dependent kinetics. [ 3 H]-ouabain binding with a 3 receptors in brain cortex and heart muscle tissues of young and adult animals had a dose-dependent character, while that in old ones had a doseindependent character. A 0.2 T static magnetic field (SMF) exposure had modulation effect on ouabain binding with a 1 , a 2 and a 3 receptors in young rats, while in adult ones, only a 3 receptors were magnetosensitive. In old animals, SMF exposure had no significant effect on ouabain binding with a 3 receptors in brain cortex, while in heart muscle, it had inhibitory effect on it. Age-dependent effect of ouabain impact on 45 Ca 2? efflux showed that all concentrations of ouabain lead to inhibitory effect in young animals' brain cortex and heart muscle (with the exception of 10 -10 and 10 -6 M), while in old ones, it had activation effect as compared with data received without ouabain. SMF exposure in young animals had activation effect on 45 Ca 2? efflux from brain cortex and heart muscle in data without ouabain, and in old rats, 45 Ca 2? efflux from brain cortex was magnetic insensitive. In old animals, SMF increased 45 Ca 2? efflux even after extra low concentration of ouabain. It is suggested that a 3 receptors having a crucial role in the regulation of Na ? /Ca 2? exchange serve as age-dependent magnetosensors of excitable cells.

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Section: Introductionmentioning

confidence: 97%

Age dependency of high-affinity ouabain receptors and their magnetosensitivity

et al. 2012

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“…Then, the question was raised-Which is the reason for the IR-induced increase in the number of a 3 ouabain receptors as compared to non-radiated cells? In previous study was shown that ouabain at high and low concentrations, leads to the increase in cAMP-dependent membrane phosphorylation causing a decrease in the membrane receptors affinity to ligand (Arvanov et al 1992;Ayrapetyan et al 2005) and the activation of Na ? /Ca 2?…”

Section: Resultsmentioning

confidence: 98%

High-affinity ouabain receptors: primary membrane sensors for ionizing radiation

2012

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Although the high sensitivity of the Na/K pump in cell membrane to ionizing radiation is well known in literature, the individual role of different isoforms of pump in determination of its radio-sensitivity is not clear yet. This is the subject of the present investigation. Using isotope, electro-physiological and enzymological methods, the effect of c-ionizing radiation on cell membrane voltagecurrent characteristics, acetylcholine-induced membrane current, 22 Na ? and 45 Ca 2? exchange between cells and bathing solution, Na?K ? -ATPase activity, dose-dependent ouabain binding with cell membrane, intracellular cAMP and membrane phosphorylation in snail neurons were studied. The changes in neurons as a result of 30-min c-radiation exposure of snails to 5.16 Ci/kg at the end of the first 30 min of post-radiation period were as follows: the increase in membrane ionic conductance reversed the ouabain sensitivity of acetylcholine-induced currents, stimulation of 22 Na ? and 45 Ca 2? uptakes, inhibition of Na/K pump, activation Na/Ca exchange in reversed mode, increase in ouabain binding with high-affinity a 3 and decrease with a 2 middle-affinity receptors, decrease in intracellular cAMP content and membrane dephosphorylation. On the basis of the obtained data, it is suggested that both a 3 and a 2 catalytic subunits of Na ? ?K ? -ATPase serve primary membrane sensors through the activation of which the biological effect of c-radiation on neurons is realized. The IR has activation effects on a 3 -dependent Na ? /Ca 2? exchange in forward and its inactivation on a 2 -dependent reverse modes.

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“…The up and down movements of the baseline of records showed the heart contraction and relaxation, respectively. The setup for the registration of heart muscle contractility was schematically represented and described in detail in Ayrapetyan et al [2005], Figure 1. 45 Ca uptake by muscles incubated in normal and H 2 O 2 -containing PS was measured by 45 Ca isotope having specific activity: 0.185-1.85 GBq/mg Ca, 5-50 mCi/mg Ca (production of Amersham Life Science, Inc., Arlington, TX).…”

Section: Methodsmentioning

confidence: 99%

Exogenous hydrogen peroxide as a possible messenger for the stimulation effect of magnetized physiological solution on heart contractility

Ayrapetyan

Dadasyan

Hayrapetyan

et al. 2008

Bioelectromagnetics

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The dual effect of magnetized physiological solution (MPS) on snail heart muscle contractility (muscle relaxation and stimulation of heartbeat) was shown previously. The MPS-induced relaxation of the heart muscle has been explained by activation of cGMP-dependent Ca(2+) effluxes from the muscle; however, the mechanism of the stimulating effect of MPS on heartbeat remains unclear. As in the presence of paramagnetic oxygen molecules, magnetic fields could generate the exogenous reactive oxygen species such as hydrogen peroxide (H(2)O(2)), we hypothesize that H(2)O(2) may play a role as the possible messengers through which the activation effect of MPS on heartbeat is realized. To test this hypothesis, the dose-dependent effects of exogenous H(2)O(2) on heart muscle contractility and (45)Ca uptake were studied. Here we compared the obtained data with the previous results of the effects of MPS on heart muscle contractility and (45)Ca uptake. We found that exogenous H(2)O(2) and MPS have similar effects on Na(+)-K(+) pump-induced transient inhibition of muscle contractility and (45)Ca uptake. The Na(+)-K(+) pump-induced depression of H(2)O(2)-sensitivity of muscle contractility is determined by activation of Ca(2+) efflux from the cell. On the basis of these data we suggest the exogenous H(2)O(2) as a possible messenger through which the stimulation effect of MPS on heart muscle is realized.

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Metabolic pathway of magnetized fluid‐induced relaxation effects on heart muscle

Cited by 20 publications

References 17 publications

Age dependency of high-affinity ouabain receptors and their magnetosensitivity

Age dependency of high-affinity ouabain receptors and their magnetosensitivity

High-affinity ouabain receptors: primary membrane sensors for ionizing radiation

Exogenous hydrogen peroxide as a possible messenger for the stimulation effect of magnetized physiological solution on heart contractility

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