Metabolic profiling of chronic obstructive pulmonary disease model rats and the interventional effects of HuaTanJiangQi decoction using UHPLC-Q-TOF/MSE

Fang, Wei; Li, ChenHui; Wu, Qingqing; Yao, Zhaomin; Wu, Jie; Huang, Peng; Wang, Dianlei; Li, Zegeng

doi:10.1016/j.jpba.2019.113078

Cited by 10 publications

(8 citation statements)

References 23 publications

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“…Several studies have found distinct differences in the metabolomic profile between patients with COPD and without COPD, and similar findings have been obtained in humans and animals [ 24 , 26 , 42 , 50 , 51 ]. Fang et al showed that 32 dysregulated metabolites in plasma are associated with the COPD status in rats [ 26 ]. Van der Does et al revealed that the levels of free alpha-linolenic acid, linoleic acid, eicosapentaenoic acid (EPA), omega-3, and EPA- and docosahexaenoic acid-derived oxylipins in the sputum of patients with COPD are noticeably lower than those in the sputum of healthy subjects [ 36 ].…”

Section: Discussionsupporting

confidence: 65%

“…Xu et al found that both the glycerophospholipids levels in both serum and the bronchoalveolar lavage fluid (BALF) are upregulated in rats with LPS-induced acute lung injury (ALI) [ 58 ]. In addition, some studies have revealed that the glycerophospholipid metabolism pathway is involved in COPD [ 22 , 26 , 61 ]. Telenga et al found that the concentrations of glycerophospholipids and fatty acids in induced sputum are significantly lower in smokers with COPD than in smokers without COPD [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

“…Mounting evidence supports the notion that metabolomics plays a hub role in the PPPM approach for different aspects of COPD [ 11 , 12 , 21 – 23 ]. Moreover, recent studies have shown that differential metabolomic profiles are associated with the COPD status in both patients and animals [ 22 , 24 – 26 ]. Cruickshank-Quinn et al reported that 2999 differential metabolites in plasma are associated with COPD outcomes, including the lung function, exacerbation frequency, degree of emphysema, and bronchodilator response (BDR) [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cruickshank-Quinn et al reported that 2999 differential metabolites in plasma are associated with COPD outcomes, including the lung function, exacerbation frequency, degree of emphysema, and bronchodilator response (BDR) [ 22 ]. Pinto-Plata V et al identified 79 differential plasma metabolites between surviving and nonsurviving COPD patients [ 25 ], and Fang W et al found that 32 differential metabolites in plasma are associated with the COPD status in rats [ 26 ]. Therefore, a comprehensive understanding of metabolomic profiles might provide insights for the PPPM strategy for COPD.…”

Section: Introductionmentioning

confidence: 99%

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Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD) severity: potential use for predictive, preventive, and personalized medicine

Zhu

Wang

et al. 2020

EPMA Journal

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Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease, and metabolomics plays a hub role in predictive, preventive, and personalized medicine (PPPM) related to COPD. This study thus aimed to reveal the role of induced sputum metabolomics in predicting COPD severity. In this pilot study, a total of 20 COPD patients were included. The induced sputum metabolites were assayed using a liquid chromatography-mass spectrometry (LC-MS/MS) system. Five oxidative stress products (myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione (GSH), neutrophil elastase (NE), and 8-iso-PGF2α) in induced sputum were measured by ELISA, and the metabolomic profiles were distinguished by principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA). The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for pathway enrichment analysis, and a significant difference in induced sputum metabolomics was observed between moderate and severe COPD. The KEGG analysis revealed that the glycerophospholipid metabolism pathway was downregulated in severe COPD. Due to the critical role of glycerophospholipid metabolism in oxidative stress, significant negative correlations were discovered between glycerophospholipid metabolites and three oxidative stress products (SOD, MPO, and 8-iso-PGF2α). The diagnostic values of SOD, MPO, and 8-iso-PGF2α in induced sputum were found to exhibit high sensitivities and specificities in the prediction of COPD severity. Collectively, this study provides the first identification of the association between induced sputum metabolomic profiles and COPD severity, indicating the potential value of metabolomics in PPPM for COPD management. The study also reveals the correlation between glycerophospholipid metabolites and oxidative stress products and their value for predicting COPD severity.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD) severity: potential use for predictive, preventive, and personalized medicine

Zhu

Wang

et al. 2020

EPMA Journal

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“…Glycerophospholipid has structural functions in bacteria, facilitates bacterial adaptation to environmental conditions, and is involved in bacteria–host interactions [28]. Glycerophospholipid is also associated with the pathophysiology of chronic obstructive pulmonary disease (COPD) [29]. These findings suggest that sn-glycerol 3-phosphoethanolamine may affect lower-airway microbiome composition and respiratory health in preterm infants.…”

Section: Discussionmentioning

confidence: 99%

The lower-airway microbiome and metabolome in preterm infants: Identifying potential predictive biomarkers of bronchopulmonary dysplasia

et al. 2020

Preprint

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The lower-airway microbiome may influence the pathogenesis of lung disease. Bronchopulmonary dysplasia (BPD) is a serious morbidity associated with preterm birth that may be influenced by lower-airway microbial or metabolic alterations. This study used16S rRNA gene sequencing, metabolomic analyses, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to investigate the lower-airway microbiome and metabolome in a cohort of preterm infants with mild, moderate, or severe BPD or no BPD. Differences in the diversity and composition of the infants' lower airway microbiota, as well as metabolic status, were initially observed, but became less pronounced at 7 days of life. Decreased diversity of the lower-airway microbiome, increased abundance of Stenotrophomonas, and increased level of sn-glycerol 3-phosphoethanolamine were associated with increased BPD severity, and have potential as predictive biomarkers for BPD. Stenotrophomonas may contribute to the development of BPD and influence the composition of the lower-airway microbiome through its metabolite, sn-glycerol 3-phosphoethanolamine. These findings provide novel insights into the lower-airway microbiome and its role in BPD.

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Serum metabolomics using ultra‐high performance liquid chromatography–Q‐Exactive tandem mass spectrometry reveals the mechanism of action of exercise training on chronic obstructive pulmonary disease rats

Tian

et al. 2022

Biomedical Chromatography

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Exercise training is the cornerstone component of pulmonary rehabilitation, which results in symptom‐reducing, psychosocial, and health economic benefits for chronic obstructive pulmonary disease (COPD) patients. However, the potential mechanisms of its action are poorly understood. This study conducted serum metabolomics using ultra‐high performance liquid chromatography–Q‐Exactive tandem mass spectrometry to determine the metabolic changes in COPD rats, and the effects of exercise training on improvement in COPD were further investigated. Twelve differential metabolites—which are primarily related to tryptophan metabolism, sphingolipid metabolism, glycerophospholipid metabolism, riboflavin metabolism, pantothenate and CoA biosynthesis, and lysine degradation—were identified in relation to COPD. After the intervention of exercise training, the levels of most metabolites were restored, and the changes in five metabolites were statistically significant, which suggested that exercise training provided effective protection against COPD and might play its role by rebalancing disordered metabolism pathways. This work enhanced our comprehension of the protective mechanism of exercise training on COPD.

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Metabolic profiling of chronic obstructive pulmonary disease model rats and the interventional effects of HuaTanJiangQi decoction using UHPLC-Q-TOF/MSE

Cited by 10 publications

References 23 publications

Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD) severity: potential use for predictive, preventive, and personalized medicine

Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD) severity: potential use for predictive, preventive, and personalized medicine

The lower-airway microbiome and metabolome in preterm infants: Identifying potential predictive biomarkers of bronchopulmonary dysplasia

Serum metabolomics using ultra‐high performance liquid chromatography–Q‐Exactive tandem mass spectrometry reveals the mechanism of action of exercise training on chronic obstructive pulmonary disease rats

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