Metabolic profiling of stages of healthy pregnancy in Hu sheep using nuclear magnetic resonance (NMR)

Sun, Lingwei; Guo, Yanliang; Fan, Yixuan; Nie, Haitao; Wang, Ruocheng; Wang, Rui

doi:10.1016/j.theriogenology.2017.01.025

Cited by 20 publications

(18 citation statements)

References 47 publications

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“…Metabolomics approaches seem in principle promising for setting up diagnostic methods that might be more convenient in specific contexts, due to the possibility to quantify a high number of molecules at the same time. However, previous studies performed in domestic animals were focused on serum [34,35], a sub-optimal sample from the point of view of non-invasivity. Therefore, despite the restricted number of samples analyzed in the present study, the obtained data can provide a preliminary urinary fingerprint of pregnancy in giraffes.…”

Section: Effect Of Pregnancymentioning

confidence: 99%

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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The urine from 35 giraffes was studied by untargeted 1H-NMR, with the purpose of obtaining, for the first time, a fingerprint of its metabolome. The metabolome, as downstream of the transcriptome and proteome, has been considered as the most representative approach to monitor the relationships between animal physiological features and environment. Thirty-nine molecules were unambiguously quantified, able to give information about diet, proteins digestion, energy generation, and gut-microbial co-metabolism. The samples collected allowed study of the effects of age and sex on the giraffe urinary metabolome. In addition, preliminary information about how sampling procedure and pregnancy could affect a giraffe’s urinary metabolome was obtained. Such work could trigger the setting up of methods to non-invasively study the health status of giraffes, which is utterly needed, considering that anesthetic-related complications make their immobilization a very risky practice.

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Section: Effect Of Pregnancymentioning

confidence: 99%

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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“…Hu sheep is a local breed that originated in the Tai Lake Valley in Southeast China [ 25 , 26 ]. It has become an important economical animals in most areas of China as it has advantages of early sexual maturation, good reproductive performance with multiple offspring, genetic stability, and good adaptability to the environment [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant

et al. 2020

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Our previous study demonstrated that a vegetable oil consisting of soybean oil, vitamin E, and ginseng saponins (SO-VE-GS) had an adjuvant effect on a foot-and-mouth disease (FMD) vaccine in a mouse model. The present study was to compare the adjuvant effects of SO-VE-GS and the conventional ISA 206 on an FMD vaccine in Hu sheep. Animals were intramuscularly (i.m.) immunized twice at a 3-week interval with 1 mL of an FMD vaccine adjuvanted with SO-VE-GS (n = 10) or ISA 206 (n = 9). Animals without immunization served as control (n = 10). Blood was sampled prior to vaccination and at 2, 4, 6, and 8 weeks post the booster immunization to detect FMD virus (FMDV)-specific IgG. Blood collected at 8 weeks after the booster was used for the analyses of IgG1 and IgG2, serum neutralizing (SN) antibody, IL-4 and IFN-γ production, and proteomic profiles. The results showed that IgG titers rose above the protection level (1:128) in SO-VE-GS and ISA 206 groups after 2 and 4 weeks post the booster immunization. At 6 weeks post the booster, the ISA 206 group had 1 animal with IgG titer less than 1:128 while all the animals in the SO-VE-GS group retained IgG titers of more than 1:128. At 8 weeks post the booster, 6 of 9 animals had IgG titers less than 1:128 with a protective rate of 33.3% in the ISA 206 group, while only 1 of 10 animals had IgG titer less than 1:128 with a protective rate of 90% in the SO-VE-GS group, with statistical significance. In addition, IgG1, IgG2, SN antibodies, IL-4, and IFN-γ in the SO-VE-GS group were significantly higher than those of the ISA 206 group. Different adjuvant effects of SO-VE-GS and ISA 206 may be explained by the different proteomic profiles in the two groups. There were 39 and 47 differentially expressed proteins (DEPs) identified in SO-VE-GS compared to the control or ISA 206 groups, respectively. In SO-VE-GS vs. control, 3 immune related gene ontology (GO) terms and 8 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were detected, while 2 immune related GO terms and 5 KEGG pathways were found in ISA 206 vs. control. GO and KEGG analyses indicated that ‘positive regulation of cytokine secretion’, ‘Th1/Th2 cell differentiation’, and ‘Toll-like receptor signaling pathways’, were obviously enriched in the SO-VE-GS group compared to the other groups. Coupled with protein–protein interaction (PPI) analysis, we found that B7TJ15 (MAPK14) was a key DEP for SO-VE-GS to activate the immune responses in Hu sheep. Therefore, SO-VE-GS might be a promising adjuvant for an FMD vaccine in Hu sheep.

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“…Additionally, genotypes of the same indel loci were distributed differently among the five sheep breeds. This may be because the tested sheep were scattered across different geographic areas [25][26][27]; environmental factors may have led to the differences in mutant frequencies and distribution. Moreover, most indels had moderate polymorphism (PIC value 0.3) in the Chinese sheep breeds.…”

Section: Discussionmentioning

confidence: 99%

“…They are characterized by many potential advantages, such as strong endurance [24], rough feedstuff resistance [25], and disease resistance [26]. Particularly, the Hu sheep is one of the most extensively distributed livestock, and its high reproductive capacity can bring huge economic benefits [27]. Owing to the regulation of phenotypic traits by PRNP, and the intron indel polymorphisms of PRNP being limited in Mongolian and Chinese sheep breeds, this study aimed to detect the potential indel loci of the sheep PRNP gene in these representative sheep breeds, and investigate their association with growth traits.…”

Section: Introductionmentioning

confidence: 99%

Genetic effects of PRNP gene insertion/deletion (indel) on phenotypic traits in sheep

Erdenee

Zhang

et al. 2018

Prion

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Prion protein (PRNP) gene is well known for affecting mammal transmissible spongiform encephalopathies (TSE), and is also reported to regulate phenotypic traits (e.g. growth traits) in healthy ruminants. To identify the insertion/deletion (indel) variations of the PRNP gene and evaluate their effects on growth traits, 768 healthy individuals from five sheep breeds located in China and Mongolia were identified and analyzed. Herein, four novel indel polymorphisms, namely, Intron-1-insertion-7bp (I1-7bp), Intron-2-insertion-15bp (I2-15bp), Intron-2-insertion-19bp (I2-19bp), and 3' UTR-insertion-7bp (3' UTR-7bp), were found in the sheep PRNP gene. In five analyzed breeds, the minor allelic frequencies (MAF) of the above indels were in the range of 0.008 to 0.986 (I1-7bp), 0.113 to 0.336 (I2-15bp), 0.281 to 0.510 (I2-19bp), and 0.040 to 0.238 (3' UTR-7bp). Additionally, there were 15 haplotypes and the haplotype 'I-D-D-D' had the highest frequency, which varied from 0.464 to 0.629 in five breeds. Moreover, association analysis revealed that all novel indel polymorphisms were significantly associated with 13 different growth traits (P < 0.05). Particularly, the influences of I2-15bp on chest width (P = 0.001) in Small Tail Han sheep (ewe), 3' UTR-7bp on chest circumference (P = 0.003) in Hu sheep, and I2-19bp on tail length (P = 0.001) in Tong sheep, were highly significant (P < 0.01). These findings may be a further step toward the detection of indel-based typing within and across sheep breeds, and of promising target loci for accelerating the progress of marker-assisted selection in sheep breeding.

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Metabolic profiling of stages of healthy pregnancy in Hu sheep using nuclear magnetic resonance (NMR)

Cited by 20 publications

References 47 publications

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant

Genetic effects of PRNP gene insertion/deletion (indel) on phenotypic traits in sheep

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