Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish

Rosa, Carlos Eduardo da; Figueiredo, Márcio Azevedo; Lanes, Carlos Frederico Ceccon; Almeida, Daniela Volcan; Monserrat, José M.; Marins, Luís Fernando

doi:10.1016/j.cbpb.2007.09.010

Cited by 59 publications

(42 citation statements)

References 35 publications

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“…GH or IGF-1 overexpression increases skeletal myocyte mitogenesis and consequently increases the metabolic rate in fishes and elevates levels of oxygen consumption [39,102]. The elevation of GH levels in GH-transgenic common carp may influence food intake and feeding behavior by up-regulating the hypothalamic orexigenic factor, agouti-related peptide 1 [103].…”

Section: Balance Between Of Somatic Growth and Metabolismmentioning

confidence: 99%

Neuroendocrine regulation of somatic growth in fishes

Dai

Zhang

Zhuo

et al. 2015

Sci. China Life Sci.

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Growth is a polygenic trait that is under the influence of multiple physiological pathways regulating energy metabolism and muscle growth. Among the possible growth-regulating pathways in vertebrates, components of the somatotropic axis are thought to have the greatest influence. There is growing body of literature focusing on the somatotropic axis and its role regulating growth in fish. This includes research into growth hormone, upstream hypothalamic hormones, insulin-like growth factors, and downstream signaling molecules. Many of these signals have both somatic effects stimulating the growth of tissues and metabolic effects that play a role in nutrient metabolism. Signals of other endocrine axes exhibit profound effects on the function of the somatotropic axis in vivo. In this review we highlight recent advances in our understanding of the teleost fish endocrine somatotropic axis, including emerging research using genetic modified models. These studies have revealed new aspects and challenges associated with regulation of the important steps of somatic growth. somatic growth, endocrine regulation, fish Citation:

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Section: Balance Between Of Somatic Growth and Metabolismmentioning

confidence: 99%

Neuroendocrine regulation of somatic growth in fishes

Dai

Zhang

Zhuo

et al. 2015

Sci. China Life Sci.

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“…Our group has recently developed a stable transgenic zebrafish (Danio rerio) model (named the F0104 lineage) which overexpresses the marine silverside (Odonthestes argentinensis) GH cDNA under strong transcriptional control of the carp (Cyprinus carpio) b-actin promoter (Figueiredo et al 2007). Using this model, Rosa et al (2008) observed an induction in the metabolic rate and a consequent increase in ROS production in the muscle of homozygous (HO) transgenic zebrafish as well as reduced expression of the glutamate cysteine ligase catalytic subunit (GCLC) gene. Considering that the liver is an important site of ADS activity, we have evaluated the effects of GH overexpression in this particular tissue, analyzing a wider range of ADSrelated genes in different genotypes of GH-transgenic zebrafish from the F0104 lineage.…”

Section: Introductionmentioning

confidence: 98%

Genotype-dependent gene expression profile of the antioxidant defense system (ADS) in the liver of a GH-transgenic zebrafish model

Rosa

Figueiredo

Lanes³

et al. 2010

Transgenic Res

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The aim of this study was to evaluate the effects of growth hormone (GH) overexpression on the gene expression profile of multiple components of the antioxidant defense system (ADS) of different genotypes of a GH-transgenic zebrafish (Danio rerio) model. Several ADS-related genes were analyzed by semiquantitative reverse transcription-PCR in the liver of hemizygous (HE) and homozygous (HO) transgenic zebrafish. The results showed a significant reduction in the glutamate cysteine ligase catalytic subunit (GCLC) and the gene expression of two glutathione S-transferase (GST) isoforms and an increase in the glutathione reductase gene in the HO group compared to non-transgenic controls. The expression of the Cu, Zn-superoxide dismutase (SOD1) and catalase (CAT) genes was reduced in HO and HE groups, respectively. Among the ten genes analyzed, two were altered in HE transgenic zebrafish and five were altered in HO transgenic zebrafish. These findings indicate a genotype-dependent gene expression profile of the ADS-related genes in the liver of our GH-transgenic zebrafish model and are in agreement with the general effects of GH hypersecretion in the fish and mouse, which involves a reduction in the capability of the tissues to deal with oxidative stress situations. The GH-transgenic zebrafish model used here seems to be an interesting tool for analyzing the effect of different GH expression levels on physiological processes.

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“…It is expected that the antioxidant system in GH-transgenic fish might be up-regulated to cope with increased ROS production based on the high metabolic demand due to accelerated growth rate in these transgenic fish. The glutathione antioxidant system was found to be enhanced in GH-transgenic coho salmon (Leggatt et al 2003); however, a resistance effect to excess GH caused a decrease in the catalytic subunit expression of glutamate-cysteine ligase, an enzyme responsible for glutathione synthesis, in GH-transgenic zebrafish (Rosa et al 2008). The reduction in the efficiency of the antioxidant defence system due to the overexpression of SLs may result in the greater susceptibility of SL-overexpressing fish to oxidative stress, and such altered stress capacity may affect their health and life span.…”

Section: Gh Slb Slamentioning

confidence: 98%

“…The decreases in these two key enzymes in the antioxidant defence system in the SL-transgenic fish suggest a reduction in the capability to deal with an oxidative stress situation (Leggatt et al 2003). An increased growth rate in transgenic zebrafish was found to be associated with an increased metabolic rate (Rosa et al 2008). It is expected that the antioxidant system in GH-transgenic fish might be up-regulated to cope with increased ROS production based on the high metabolic demand due to accelerated growth rate in these transgenic fish.…”

Section: Gh Slb Slamentioning

confidence: 99%

A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae

Wan¹,

Chan²

2010

Journal of Molecular Endocrinology

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Somatolactin (SL) is a fish-specific hormone that belongs to the prolactin (PRL) and GH family. Recently, two forms of SL, SLa and SLb, have been found in some species, and may have different actions and functions. To investigate the role of SL in fish growth and metabolism, we generated transgenic fish founders with ectopic expression of SLa and SLb to study the physiological functions and actions of these SLs among several marker genes. We fused the cDNAs encoding the precursor SLs in frame to a zebrafish b-actin gene promoter to generate transgenic zebrafish lines that were coinjected with a green fluorescent protein (GFP) driven by the same promoter. The transgenic zebrafish were selected based on GFP expression and confirmed by genomic PCR, Southern blot analysis, and transgene expression. Investigations into the expression of marker genes in larvae on different pathways using real-time PCR have provided a general understanding of the actions of SLs. This study found that the overexpression of SLa and SLb in vivo significantly enhanced the transcription of IGFs, insulin, leptin, sterol regulatory element binding protein 1, and fatty acid synthase, as well as the expression level of vitellogenin and proopiomelanocortin, while causing reduced levels of catalase and glutathione S-transferase in the larvae of transgenic zebrafish.

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Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish

Cited by 59 publications

References 35 publications

Neuroendocrine regulation of somatic growth in fishes

Neuroendocrine regulation of somatic growth in fishes

Genotype-dependent gene expression profile of the antioxidant defense system (ADS) in the liver of a GH-transgenic zebrafish model

A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae

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