Metabolic rate of blue mussels (Mytilus edulis) under varying post-harvest holding conditions

Abstract

The mussel (Mytilus edulis) is successfully grown in aquaculture in Europe. Mussels are usually sold live and wet storage is becoming more common. In this study, oxygen demand and ammonia excretion were assessed at increasing water temperatures and different post-harvest situations. This information was used to calculate minimal flow rates per unit biomass of live mussels sufficient to keep oxygen above 5 mg L −1 or 50% saturation, and avoid accumulation of ammonia in commercial wet storage. In this study, rope-grown mussels were kept out of water for 8 h to simulate harvesting conditions and then re-immersed in holding tanks at 5, 10 and 15 • C. Oxygen and ammonia concentrations were measured immediately after mussels were re-immersed (0 h), after 6 h and then every day for 3 days. After this period, the mussels were again kept out of water for 48 h to simulate long-distance transport and once again re-immersed for the same period as before. In the first 6 h after re-immersion, the oxygen consumption was between 7.5 and 12.2 μmol g −1 h −1 (dry flesh) and after this period it decreased to a standard level of around 4.0 ± 0.9 μmol g −1 h −1 and was independent of temperature. There were no major differences in oxygen consumption between mussels having spent 8 and 48 h out of water at any of the subsequent water temperatures used for re-immersion. In contrast, the ammonia excretion showed greater differences according to temperature and time out of water. Ammonia excretion was lowest at 5 • C (<0.01 μmol g −1 h −1). The implications of these results for the industry and authorities are discussed considering the water flow rate, depuration specifications and energy costs.

Cockle (Anadara granosa) Tolerance to Ammonia Exposed to Various Concentrations

“…The duration of exposure to ammonia was 96 hours and observation recorded hourly. Cockles were not fed during the observation to reduce ammonia production/interference [23]. The death of the cockles was confirmed by its inability to close the valves upon mechanical stimulus such as touching it with glass rod [24] and immediately removed from the container.…”

A GC-MS-based metabolomics investigation on scallop (Chlamys farreri) during semi-anhydrous living-preservation

“…Aquatic products are not an exception, but even more restricted regarding freshness. Living preservation and transportation have become hot spots of investigation to increase the quality and value of the aquatic product supply chain (Anacleto, et al, 2013;Barrento, Lupatsch, Keay, & Christophersen, 2013;Barrento, Marques, Pedro, Vaz-Pires, & Nunes, 2008;Fotedar & Evans, 2011). Transportation and preservation of live aquatic animals in waterless conditions were developed Cho, Kim, Lee, & Choi, A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 6 technology.…”

“…Transportation and preservation of live aquatic animals in waterless conditions were developed Cho, Kim, Lee, & Choi, A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 6 technology. Conventional optimizing methods, based on limited information revealed by physiology indexes (Anacleto, et al, 2013;Barrento, et al, 2013;Barrento, et al, 2008;Fotedar, et al, 2011;Funatsu, Kawasaki, Usui, Nakategawa, Shimizu, & Abe, 2007), are usually superficial and aimless. We utilized a metabolomics approach to establish practical guidance for optimization of preservation and transportation parameters and discover the mechanisms of the stresses the scallops suffered.…”

The effect of transportation and re-watering strategies on the survival, physiology and batch weight of the blue mussel, Mytilus edulis

“…Different numbers denote statistical significance at least P b 0.05%, inside discrete supply chain stage. strategy, which we have termed "overpacking" (Barrento et al, 2013b). Overall, in this study, depurated mussels lost less weight during transport (3%) than the numbers reported by mussel farmers (8%).…”

“…fresh smell). To improve the industry it is essential to understand the ecology and physiology of M. edulis and relate it to commercial practices and economic demand particularly for the rope grown mussel sub-sector (Barrento et al, 2013a(Barrento et al, , 2013bGallardi et al, 2014). In this study we compared husbandry practices, at pilot commercial scale, for rope grown M. edulis before, during and after transport, in order to inform and improve the quality, quantity and efficiency.…”