Metabolic Response of Faecalibacterium prausnitzii to Cell-Free Supernatants from Lactic Acid Bacteria

Abstract

Interest in preventive or therapeutic strategies targeting gut microbiota is increasing. Such strategies may involve the direct replenishment of the gut microbiota with single strains or strain mixtures, or the manipulation of strain abundance through dietary intervention, including lactic acid bacteria. A few candidate species associated with health benefits have been identified, including Faecalibacterium prausnitzii. Given its growth requirements, modulation of this bacterium has not been extensively studied. In this investigation, we explored the capacity of cell-free supernatants of different Lactobacillus, Streptococcus, Lactococcus, and Bifidobacterium strains to stimulate the growth of F. prausnitzii A2-165. Modulation by four strains with the greatest capacity to stimulate growth or delay lysis, Lactococcus lactis subsp. lactis CNCM I-1631, Lactococcus lactis subsp. cremoris CNCM I-3558, Lactobacillus paracasei CNCM I-3689, and Streptococcus thermophilus CNCM I-3862, was further characterized by transcriptomics. The response of F. prausnitzii to cell-free supernatants from these four strains revealed several shared characteristics, in particular, upregulation of carbohydrate metabolism and cell wall-related genes and downregulation of replication and mobilome genes. Overall, this study suggests differential responses of F. prausnitzii to metabolites produced by different strains, providing protection against cell death, with an increase in peptidoglycan levels for cell wall formation, and reduced cell mobilome activity.

Metagenome-Predicted Growth Rate And Metatranscriptomic Analysis Reveal A Slow Growth Rate Of And High Butyrate Formation By Faecalibacterium Prausnitzii In The Rumen Of Low Methane-Emitting Sheep

“…Nevertheless, the upregulated expression of the F. prausnitzii genes involved in carbohydrate metabolism is consistent with an in-vitro study [50], which showed that carbohydrate metabolism was upregulated while DNA replication was downregulated in F. prausnitzii when expressed to cell-free supernatant of lactic acid bacteria. Although the present study did not detect any signi cant downregulation of growthrelated genes of F. prausnitzii, the lower growth rate of F. prausnitzii in the LMY sheep than in the HMY sheep suggests that the growth-related genes of F. prausnitzii were downregulated in the LMY sheep.…”

“…The lactate concentration in the rumen of the LMY sheep was much higher than in the HMY sheep (~ 0.9 vs. 0.014 mM) [8]. F. prausnitzii, as shown in the present study and the study by Lebas M, et al [50], might adjust its metabolism towards carbohydrate metabolism when exposed to lactate. Its ability to utilize lactate [46] might be one plausible explanation.…”

Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise

“…Conversely, both were detected by species-specific PCR in preliminary acute ingestion assays in feces (data not shown), although these results cannot be directly extrapolated to humans. Discussing a possible explanation for the functional effect of the formulation, even with a decrease in lactobacilli relative levels, is the potential capacity of extracellular metabolites of lactic acid bacteria to act as a prebiotic for key bacteria, influencing not only their growth and cell death, but also the expression of genes related to cell protection [ 54 ]. However, it seems that changes in microbiome do not directly correlate with the strains consumed, pointing that other mechanisms not necessary based on simple colonization might have a role on the results obtained.…”

Intraspecific Diversity of Microbial Anti-Inflammatory Molecule (MAM) from Faecalibacterium prausnitzii

“…By contrast, we observed strong divergence in the sequences upstream of the RBS. From recently published RNAseq data [20], we determined that transcription of the mam gene begins 110-bp upstream of its translational start site. This region contains a TATA box, where formation of the transcription pre-initiation complex takes place (Figure 2).…”