Metabolic responses in Scophthalmus maximus kidney subjected to thermal stress

Yang, Shuangshuang; Zhao, Tingting; Ma, Aijun; Huang, Zhihui; Liu, Zhifeng; Cui, Wenxiao; Zhang, Jinsheng; Zhu, Chunyue; Guo, Xiaoli; Yuan, Chenhao

doi:10.1016/j.fsi.2020.04.003

Cited by 35 publications

(16 citation statements)

References 57 publications

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“…This effect was previously observed in other teleost fish under stress conditions, including the plasmatic activity of ALT and AST under high densities and metal pollution in rohu (Labeo rohita) [39,40], metal toxicity and pesticides in Nile tilapia (Oreochromis niloticus) [41,42] and spotted snakehead (Channa punctatus) [43], and elevated ALT, AST and AP under handling stress in red cusk-eel [25]. In terms of high-temperature stress, different results have been observed, with an increase in the plasmatic activity of AST and ALT in pufferfish (Takifugu obscurus) [44], which is consistent with our results for red cusk-eel, while no effect for AST and ALT was observed in Turbot (Scophthalmus maximus) [45] under high-temperature stress, evidencing that the hepatic response to thermal stress could vary according to fish species. The increased levels on ALT, AST an AP enzymes could be indicative of liver dysfunction, reflecting hepatocyte damage due to thermal stress, which is concordant with the results observed in mammals [46].…”

Section: High-temperature Effect On Hepatic Enzymessupporting

confidence: 87%

High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

Dettleff

Zuloaga

Fuentes

et al. 2022

Biology

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Environmental stressors, such as temperature, are relevant factors that could generate a negative effect on several tissues in fish. A key fish species for Chilean aquaculture diversification is the red cusk-eel (Genypterus chilensis), a native fish for which knowledge on environmental stressors effects is limited. This study evaluated the effects of high-temperature stress on the liver of red cusk-eel in control (14 °C) and high-temperature (19 °C) groups using multiple approaches: determination of plasmatic hepatic enzymes (ALT, AST, and AP), oxidative damage evaluation (AP sites, lipid peroxidation, and carbonylated proteins), and RNA-seq analysis. High-temperature stress generated a significant increase in hepatic enzyme activity in plasma. In the liver, a transcriptional regulation was observed, with 1239 down-regulated and 1339 up-regulated transcripts. Additionally, high-temperature stress generated oxidative stress in the liver, with oxidative damage and transcriptional modulation of the antioxidant response. Furthermore, an unfolded protein response was observed, with several pathways enriched, as well as a heat shock response, with several heat shock proteins up regulated, suggesting candidate biomarkers (i.e., serpinh1) for thermal stress evaluation in this species. The present study shows that high-temperature stress generated a major effect on the liver of red cusk-eel, knowledge to consider for the aquaculture and fisheries of this species.

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Section: High-temperature Effect On Hepatic Enzymessupporting

confidence: 87%

High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

Dettleff

Zuloaga

Fuentes

et al. 2022

Biology

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“…In the fish, kidney tissues have a crucial role in stress responses and usually require mobilization of energy substrates to deal with stress (Costas et al ., 2011; Yang et al ., 2020; Zhou et al ., 2011). Nonetheless, in P. bifasciatus kidney, there was no variation in many enzymes associated with energy metabolism, such as HK, PFK, PK, LDH, MDH and CS, under high temperature stress.…”

Section: Discussionmentioning

confidence: 99%

“…In fish, amino acids constitute a main substrate for energy production and are derived from the diet through protein breakdown (Bever et al ., 1981; Jia et al ., 2017; Yang et al ., 2020). The transamination or deamination of amino acids facilitates the gluconeogenesis pathway (Dalvi et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In freshwater fish, the kidney is important as it removes water that passively enters the gill through the generation of dilute urine (Martinez, 2017). In addition, fish kidneys are part of the hypothalamic–pituitary–interrenal axis, and its activation is essential for the maintenance of vital functions in response to stressful events (Yang et al ., 2020). Although fish gills represent the primary sites for acid–base regulation in fish, the kidney also plays an essential role in modulating HCO 3 − levels in plasma by reabsorbing the filtered and accumulated HCO 3 − (Martinez, 2017; Perry & Gilmour, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Effects of heat shock on energy metabolism and antioxidant defence in a tropical fish speciesPsalidodon bifasciatus

Resende

Pereira

Schleger

et al. 2022

Journal of Fish Biology

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Predictions about global warming have raised interest in assessing whether ectothermic organisms will be able to adapt to these changes. Understanding the physiological mechanisms and metabolic adjustment capacity of fish subjected to heat stress can provide subsidies that may contribute to decision-making in relation to ecosystems and organisms subjected to global climate change. This study investigated the antioxidant defence system and energy metabolism of carbohydrate and protein responses in the gill, liver and kidney tissues of Psalidodon bifasciatus (Garavello & Sampaio 2010), a Brazilian freshwater fish used in aquaculture and in biological studies, following exposure to heat shock at 31 C for 2, 6, 12, 24 and 48 h. The fish pres-

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“…Therefore, researchers have begun to pay attention to the effects of heat stress on turbot. Yang et al (2020) found through metabolomics analysis that the maintenance of renal aerobic metabolism and activation of gluconeogenesis are important metabolic strategies of turbot against heat stress. Based on both transcriptomic and proteomic analyses, Yue et al (2018) identified a number of differentially expressed molecular targets in immune‐stimulated turbot at high temperatures.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and structural characterization of PPARα gene from turbot ( Scophthalmus maximus ) and functional exploration of lipid metabolism in response to thermal stress

Zhao

Huang

et al. 2022

Aquaculture Research

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Peroxisome proliferator‐activated receptor alpha (PPARα), as a transcription factor, plays an important role in the regulation of lipid metabolism. To better understand the regulatory role of PPARα in lipid metabolism in turbot, we analyzed the sequence structure of PPARα and its stress response to a high temperature. We also investigated the regulation of genes related to lipid metabolism after the knockdown of PPARα. The CDS of PPARα was 1410 bp, encoding 469 amino acids. The relative molecular weight of the protein was 52.64 kDa, and the predicted pI was 5.63. The architecture of the PPARα gene exhibited a ZnF‐C4 domain and a HOLI domain. Phylogenetic analysis indicated that PPARα has been highly conserved during evolution. The qPCR results showed that the mRNA level of PPARα was significantly increased, and the expression of the lipid synthesis genes FASN and SCD was significantly downregulated under high‐temperature stress. PPARα interference and inhibition results showed that the expression levels of FASN and SCD were significantly increased. Our results suggest that high temperatures activate PPARα and inhibit lipid synthesis by downregulating the expression of FASN and SCD in order to reduce lipid deposition and maintain lipid metabolism disorder caused by high‐temperature stress. This is the first report of PPARα in turbot. It will help for studying the evolution of PPARα and provide a reliable reference for studying the regulation mechanism of lipid metabolism in fish under stress.

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Metabolic responses in Scophthalmus maximus kidney subjected to thermal stress

Cited by 35 publications

References 57 publications

High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

Effects of heat shock on energy metabolism and antioxidant defence in a tropical fish speciesPsalidodon bifasciatus

Molecular cloning and structural characterization of PPARα gene from turbot ( Scophthalmus maximus ) and functional exploration of lipid metabolism in response to thermal stress

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