Metabolic stress in dry cows and nets in development of subclinical ketosis

Авдеенко, В.С.; Kalyuzhny, Ivan; Tresnitsky, S.N.

doi:10.30896/0042-4846.2019.22.2.36-41

Cited by 2 publications

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“…На фоне роста продуктивности у молочных коров обострилась проблема распространения бесплодия [8,9]. Основными причинами снижения воспроизводительной функции у крупного рогатого скота, как правило, являются дисгормональные расстройства, которые возникают на фоне метаболических нарушений и патологий органов внутренней секреции [3,5,6]. Поэтому оценка гуморального статуса животных является одним из способов прогнозирования бесплодия и выбора объективной тактики коррекции гормональных нарушений [4,7,10].…”

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Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Nikolaev¹

2022

asj

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The optimal conditions for determining the concentration of progesterone in the blood plasma of cattle were selected by high-performance liquid chromatography-tandem mass spectrometry, and the relationship of the level of this progestin with fertilization after treatment of cows with hormonal preparations was studied. It was found that the time of progesterone peak release under the selected chromatography mode is within 2.7 minutes, and the optimal precursor ion is [M+H]+ m/z=315.3. The developed technique makes it possible to determine the endogenous level of the steroid in the blood of cattle with high accuracy (up to hundredths of ng/ml). Analysis of progesterone concentration in cows at the time of the final injection of the prostaglandin analogue showed that animals with different reproductive status have no significant differences in the content of this steroid. The most statistically significant indicators were obtained on the day of insemination. Thus, in infertile cows, the hormone concentration was 1.9...4.2 times lower than in fertilized cows (P<0.05). The higher steroid content in fertilized animals at the time of insemination most likely reflects the ovarian response to the preovulatory release of luteinizing hormone and the readiness of the follicle for ovulation. Keywords: cattle; high-performance liquid chromatomass spectrometry; progesterone; synchronization of sexual cyclicity; fertilization; infertility.

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Section: Original Articleunclassified

Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Nikolaev¹

2022

asj

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Ketosis and its role in Bos taurus reproductive impairment

SHIRYAEV

Владимирович²,

Stanislavovich

et al. 2020

Vestn. Ross. univ. družby nar., Ser. Agron. životnovod.

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The analysis of relevant and significant scientific research on the physiological and biochemical aspects of ketosis, and the main mechanisms of influence on reproductive function in this metabolic pathology is presented. Strengthening breeding potential and creating conditions for its implementation through modern feeding and maintenance technologies led to the spread of diseases of non-communicable etiology, including ketosis diseases. Ketotic diseases cause various disorders in carbohydrate-lipid, protein, water-electrolyte and vitamin-mineral metabolism. The monitoring of studies on the biological role of ketosis showed its effect on animal fertility, quality of female gametes and fetal development. When considering ketosis, it is important to understand precisely the mechanisms of influence, since many methods for diagnosing and combating ketosis diseases are based on their knowledge. The key mechanisms of ketone bodies formation, the reasons for increase in their concentration were considered. It is of great practical importance for development of diagnostic tests that make it possible to determine the direction of disturbances in energy and plastic processes. Based on the data presented in the studies, conclusions were drawn about the role of -hydroxybutyric acid as a direct factor in reducing the reproductive function of Bos taurus .

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Metabolic stress in dry cows and nets in development of subclinical ketosis

Cited by 2 publications

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Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Determination of progesterone levels in the blood of cattle by liquid chromatography-tandem mass spectrometry and the relationship of steroid concentration with fertilization

Ketosis and its role in Bos taurus reproductive impairment

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