Metabolism in Myocutaneous Flaps Studied by in Situ Microdialysis

Röjdmark, Jonas; Blomqvist, Lennart; Malm, Maj; Adams-Ray, Bill; Ungerstedt, Urban

doi:10.1080/02844319850158921

Cited by 56 publications

(39 citation statements)

References 20 publications

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“…The procedure has reached widespread application in neurosurgery 12,13 and the neurointensive care unit, 14-16 cardiovascular 17 and plastic surgery. 18,19 Furthermore, it allows monitoring metabolism in skeletal muscle, 20,21 adipose tissue, [22][23][24] and tissue glucose in diabetes mellitus diseases. 25 It also has been used to monitor liver grafts in rats.…”

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confidence: 99%

Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig model

Nowak

Ungerstedt

Wernerman

et al. 2002

Liver Transplantation

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Microdialysis provides the opportunity to continuously monitor metabolic changes in tissue. The aim of the study is to monitor metabolic changes in the liver graft over time during transplantation in a pig model. Fourteen littermate female pigs with a body weight of 30 to 34 kg were used for seven orthotopic liver transplantations. Intrahepatic implantation of a microdialysis catheter into the liver graft was performed in the donor. Microdialysis samples were collected at 20-minute intervals during the donor operation, cold preservation, and for 7 hours after reperfusion in the recipient. Glucose, lactate, pyruvate, and glycerol concentrations were measured. After cold perfusion, glucose, lactate, and glycerol levels increased, whereas pyruvate levels decreased rapidly. During cold storage, glucose and glycerol levels increased, whereas lactate levels remained stable and pyruvate levels were undetectable. During implantation of the liver graft, glucose, lactate, and glycerol levels showed an accelerated increase. After portal reperfusion, glucose, lactate, and glycerol levels continued to increase for another 40 to 60 minutes, after which they decreased and finally settled at normal levels. At this time, pyruvate levels increased, with a peak within 2 hours after reperfusion, and then decreased to normal levels. Calculated lactate-pyruvate ratio increased after cold perfusion and remained stable during cold storage. During rewarming, it showed an accelerated increase, but after reperfusion, it decreased rapidly. Rewarming and reperfusion are most harmful to the liver, reflected by an accelerated increase in glucose and glycerol levels and lactate-pyruvate ratio. High intrahepatic glucose levels during ischemia appear to be a liver-specific event, which may represent glycogen degradation in injured hepatocytes. (Liver Transpl 2002;8:424-432.)

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confidence: 99%

Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig model

Nowak

Ungerstedt

Wernerman

et al. 2002

Liver Transplantation

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“…Microdialysis has been utilized previously for muscle metabolic studies at rest, where 20 min of total ischaemia by the use of a tourniquet approximately doubled the microdialysate lactate concentration [10], also during static exercise where microdialysate lactate concentrations increased with increasing contraction force [11] and under perioperative ischaemic conditions in skin flaps [12]. The technique has also recently been used in dynamically exercising muscle where it was shown that microdialysate lactate and glucose recovery, i.e.…”

Section: Discussionmentioning

confidence: 99%

Erratum to: Lactate concentrations in human skeletal muscle biopsy, microdialysate and venous blood during dynamic exercise under blood flow restriction

Lundberg

Olofsson

Ungerstedt

et al. 2001

Pflugers Arch - Eur J Physiol

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The intramuscular microdialysate lactate concentration during dynamic exercise with various degrees of blood flow restriction and its relation to lactate concentration in skeletal muscle biopsy and venous blood were studied. Nine healthy males performed three one-legged knee extension exercises (Ex 1-3). Blood flow was restricted stepwise by applying supra-atmospheric pressure over the working leg. Microdialysate mean (range) lactate concentrations at the end of the exercise periods were 3.2 (0.5-6.6), 4.4 (1.1-9.8) and 7.9 (1.1-11.6) mmol·l -1 during unrestricted, moderately restricted and severely restricted blood flow respectively. There was a significant correlation between microdialysate and venous lactate concentrations at the end of all three exercise periods. Microdialysate lactate concentration correlated significantly to skeletal muscle biopsy lactate concentration at the end of Ex 1. In conclusion, microdialysate lactate concentration in the 1 working muscle increased step-wise with increasing blood flow restriction. It showed a better correlation to venous than to muscle biopsy lactate, which is possibly partly explained by the characteristics of diffusion between body compartments and differences in time resolution between the methods used.Keywords Human · Ischaemia · Lactate · Microdialysis · Muscle biopsy An erratum to this article can be found at http://dx

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“…Various techniques have been used to demonstrate oxygen delivery [9], oxygen homeostasis or blood flow by dye diffusion [10], laser Doppler flowmetry [11], oxygen measurements [12,13] or microdialysis [14,15,16]. However, all these techniques have their limitations in assessing blood perfusion only indirectly.…”

Section: Introductionmentioning

confidence: 99%

Morphology and Hemodynamics during Vascular Regeneration in Critically Ischemic Murine Skin Studied by Intravital Microscopy Techniques

Schweizer

Merz²,

Schlosser³

et al. 2011

Eur Surg Res

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Background: With the understanding of angiogenesis and arteriogenesis, new theories about the orchestration of these processes have emerged. The aim of this study was to develop an in vivo model that enables visualization of vascular regenerating mechanisms by intravital microscopy techniques in collateral arteriolar flap vascularity. Methods: A dorsal skin flap (15 × 30 mm) was created in mice and fixed into a skinfold chamber to allow for assessment of morphology and microhemodynamics by intravital fluorescence microscopy (IVFM). Laser scanning confocal microscopy (LSCM) was utilized for three-dimensional reconstruction of the microvascular architecture. Results: Flap tpO₂ was 5.3 ± 0.9 versus 30.5 ± 1.2 mm Hg in controls (p < 0.01). The collateral arterioles in the flap tissue were dilated (29.4 ± 5.3 µm; p < 0.01 vs. controls) and lengthened in a tortuous manner (tortuosity index 1.00 on day 1 vs. 1.35± 0.05 on day 12; p < 0.01). Functional capillary density was increased from 121.00 ± 25 to 170 ± 30 cm/cm² (day 12; p < 0.01) as a result of angiogenesis. Morphological evidence of angiogenesis on capillary level and vascular remodeling on arteriolar level could be demonstrated by IVFM and LSCM. Conclusions: Present intravital microscopy techniques offer unique opportunities to study structural changes and hemodynamic effects of vascular regeneration in this extended axial pattern flap model.

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Metabolism in Myocutaneous Flaps Studied by in Situ Microdialysis

Cited by 56 publications

References 20 publications

Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig model

Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig model

Erratum to: Lactate concentrations in human skeletal muscle biopsy, microdialysate and venous blood during dynamic exercise under blood flow restriction

Morphology and Hemodynamics during Vascular Regeneration in Critically Ischemic Murine Skin Studied by Intravital Microscopy Techniques

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