Metabolism of 4-hydroxynonenal, a cytotoxic product of lipid peroxidation, in rat precision-cut liver slices

Laurent, Alexia; Perdu-Durand, E.; Alary, J.; Debrauwer, Laurent; Cravedi, Jean‐Pierre

doi:10.1016/s0378-4274(99)00301-x

Cited by 68 publications

(57 citation statements)

References 29 publications

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“…Glutathione conjugates are removed outside the cell, and this is one of the stages of detoxication of xenobiotics. That is also the way for binding and removing lipid peroxidation products [10,11,50]. Methionine did not have an advantageous influence upon the system of glutathione enzymes.…”

Section: Discussionmentioning

confidence: 95%

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Influence of Fluoride on Rat Kidney Antioxidant System: Effects of Methionine and Vitamin E

Błaszczyk

Grucka-Mamczar

Kasperczyk

et al. 2007

Biol Trace Elem Res

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The aim of the study has been to determine and compare the influence upon the kidney antioxidative system, exercised by administration of vitamin E, and vitamin E in combination with methionine, under conditions of oxidative stress induced by sodium fluoride. The experiment was carried out on Wistar FL rats (adult males) that, for 35 days, were administered water, NaF, NaF with vitamin E, or vitamin E with methionine (doses: 10 mg NaF/kg of body mass/24 h, 3 mg vitamin E per 10 microl per rat for 24 h, 2 mg methionine per rat for 24 h). The influence of administered sodium fluoride and antioxidants upon the antioxidative system in kidney was examined by analyzing the concentration of malondialdehyde (MDA) and the activity of the most important antioxidative enzymes (SOD, total and both its isoenzymes, GPX, GST, GR, and CAT). The studies carried out confirmed the disadvantageous effect of the administered dose of NaF upon the antixodiative system in rats (increase in the concentration MDA, decrease activity of all antioxidative enzymes). The administration of vitamin E increased the activity of studied enzymes with the exception of glutathione reductase GR; it also reduced the processes of lipid peroxidation. It has been found that combined doses of vitamin E and methionine were most effective in inhibiting lipid peroxidation processes. The results confirmed the antioxidative properties of methionine.

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Section: Discussionmentioning

confidence: 95%

“…The glutathione system (glutathione, GSH, GPX, and GR) is a key defense against hydrogen peroxide and other peroxides [7,9]. Glutathione transferases participate, among other things, in removal of lipid peroxidation products [10,11].…”

mentioning

confidence: 99%

Influence of Fluoride on Rat Kidney Antioxidant System: Effects of Methionine and Vitamin E

Błaszczyk

Grucka-Mamczar

Kasperczyk

et al. 2007

Biol Trace Elem Res

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“…Under oxidative stress conditions, reactive oxygen species induce DNA fragmentation, cellular injury, protein damage and lipid peroxidation [36,37]. In addition, isoniazid/ rifampicin-induced rats exhibited significantly declined renal GSH content which is a potent antioxidant forming S-conjugates with products of lipid peroxidation [38]. Furthermore, activity of the antioxidant enzymes SOD and GPx was declined in kidneys of the isoniazid/rifampicin-induced rats.…”

Section: Discussionmentioning

confidence: 99%

Ruta graveolens Protects Against Isoniazid/Rifampicin-Induced Nephrotoxicity through Modulation of Oxidative Stress and Inflammation

M¹

2016

Glob J Biotechnol Biomater Sci

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Background and Aim: Drug-induced nephrotoxicity is a renal dyfunction that arises as a result of exposure to nephrotoxic drugs. Anti-tuberculosis therapy can cause nephrotoxicity and permanent kidney damage. The current study was designed to evaluate the possible protective effects of Ruta graveolens L. leaves extract against isoniazid/rifampicin-induced nephrotoxicity in rats. Methods:The experimental rats received isoniazid and rifampicin at dose level of 50 mg/kg, and 50 or 100 mg/kg/day Ruta graveolens leaves extract orally for 45 days.Results: Isoniazid/rifampicin administration induced kidney injury evidenced by the histopathological alterations as well as significant (P<0.001) increase in serum creatinine, urea and uric acid. Isoniazid/rifampicin-intoxicated rats exhibited a significant increase in serum tumor necrosis factor alpha (P<0.001), and renal lipid peroxidation (P<0.01) and nitric oxide (P<0.001) levels. On the other hand, reduced glutathione level, and activity of superoxide dismutase and glutathione peroxidase were markedly (P<0.001) declined in kidney of isoniazid/rifampicin-induced rats. Concomitant supplementation with either 50 or 100 mg/kg dose of Ruta graveolens leaves extract prevented the isoniazid/rifampicin-induced biochemical and histopathological alterations. Conclusion:The present investigation confers new information on the protective mechanism of Ruta graveolens leaves extract on anti-tuberculosis therapy-induced nephrotoxicity. Ruta graveolens protects against isoniazid/rifampicin-induced renal injury through attenuation of inflammation and oxidative stress, and potentiation of the antioxidant defense system.

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“…2). Such Michael adducts have also been observed with peptides containing thiol groups such as glutathione (GSH), whose reaction with HNE is a well-recognized pathway of detoxification in those biological systems (e.g., erythrocytes, liver tissue) where GSH concentrations are particularly high (Laurent et al, 2000;Srivastava et al, 2000). Anyway, the facile reactivity of HNE with thiols, which makes it an ideal substrate for GSH conjugation, results in the depletion of cellular GSH and disruption of cellular redox status.…”

Section: B Hne Reactivity and Reaction Mechanisms With Target Proteinsmentioning

confidence: 99%

Mass spectrometry for detection of 4‐hydroxy‐trans‐2‐nonenal (HNE) adducts with peptides and proteins

Carini

Aldini

Facino

2004

Mass Spectrometry Reviews

167

157

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Despite the great technical advancement of mass spectrometry, this technique has contributed in a limited way to the discovery and quantitation of specific/precocious markers linked to free radical-mediated diseases. Unsaturated aldehydes generated by free radical-induced lipid peroxidation of polyunsaturated fatty acids, and in particular 4-hydroxy-trans-2 nonenal (HNE), are involved in the onset and progression of many pathologies such as cardiovascular (atherosclerosis, long-term complications of diabetes) and neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, and cerebral ischemia). Most of the biological effects of HNE are attributed to the capacity of HNE to react with the nucleophilic sites of proteins and peptides (other than nucleic acids), to form covalently modified biomolecules that can disrupt important cellular functions and induce mutations. By considering the emerging role of HNE in several human diseases, an unequivocal analytical approach as mass spectrometry to detect/elucidate the structure of protein-HNE adducts in biological matrices is strictly needed not only to understand the reaction mechanism of HNE, but also to gain a deeper insight into the pathological role of HNE. This with the aim to provide intermediate diagnostic biomarkers for human diseases. This review sheds focus on the "state-of-the-art" of mass spectrometric applications in the field of HNE-protein adducts characterization, starting from the fundamental early studies and discussing the different MS-based approaches that can provide detailed information on the mechanistic aspects of HNE-protein interaction. In the last decade, the increases in the accessible mass ranges of modern instruments and advances in ionization methods have made possible a fundamental improvement in the analysis of protein-HNE adducts by mass spectrometry, and in particular by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) tandem mass spectrometry. The recent developments and uses of combined analytical approaches to detect and characterize the type/site of interaction have been highlighted, and several other aspects, including sample preparation methodologies, structure elucidation, and data analysis have also been considered.

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Metabolism of 4-hydroxynonenal, a cytotoxic product of lipid peroxidation, in rat precision-cut liver slices

Cited by 68 publications

References 29 publications

Influence of Fluoride on Rat Kidney Antioxidant System: Effects of Methionine and Vitamin E

Influence of Fluoride on Rat Kidney Antioxidant System: Effects of Methionine and Vitamin E

Ruta graveolens Protects Against Isoniazid/Rifampicin-Induced Nephrotoxicity through Modulation of Oxidative Stress and Inflammation

Mass spectrometry for detection of 4‐hydroxy‐trans‐2‐nonenal (HNE) adducts with peptides and proteins

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