Metabolism of Bovine Semen. IX. Glutamic-Oxaloacetic and Glutamic-Pyruvic Transaminase Activities

Flipse, R.J.

doi:10.3168/jds.s0022-0302(60)90237-x

Cited by 47 publications

(19 citation statements)

References 10 publications

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“…Our data agree with the findings of Flipse (1960) that the GOT activity of bull seminal plasma, although high (Roussel and Stallcup 1965b), is distinctly lower than in the spermatozoa. Much of the GOT activity in the seminal plasma ofthe ram clearly comes from the epididymal fluid (Alumot and Schindler 1965) and seminal vesicles.…”

Section: (E) Experiments 5: Effect Of Cold Shock and Deep Freezingsupporting

confidence: 83%

Studies of the Distribution and Source of Enzymes in Mammalian Semen

Murdoch¹,

White²

1968

Aust. Jnl. Of Bio. Sci.

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SummarySuccinate dehydrogenase (SDH), lactate dehydrogenase (LDH), glutamateoxaloacetate transaminase (GOT), and acid and alkaline phosphatase are present in ram, bull, dog, rabbit, and human semen but the concentrations differ from one species to another. Amylase is found in the semen of all these species except the ram, and glucose·6-phosphate dehydrogenase (GDH) in all except the bull.Studies of the ram and bull showed that if a particular enzyme is present in whole semen, it occurs in both the spermatozoa and seminal plasma but there was often a great difference in the activity of the two fractions, e.g.· SDH and LDH activity was greater in the spermatozoa than in the plasma. SDH appears to be very tightly bound in ram spermatozoa since, unlike LDH, it is not released by sonic disruption. LDH, GOT, GDH, and acid phosphatase are leached out of ram and bull spermatozoa by sudden cooling (cold shock) or deep freezing.The enzyme content of ram testicular fluid is low compared with epididymal and vesicular fluids and some of the enzymes found in the seminal plasma probably have their origin in the epididymis and seminal vesicles.

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Section: (E) Experiments 5: Effect Of Cold Shock and Deep Freezingsupporting

confidence: 83%

Studies of the Distribution and Source of Enzymes in Mammalian Semen

Murdoch¹,

White²

1968

Aust. Jnl. Of Bio. Sci.

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“…Two principal transaminases were investigated: glutamic-aspartic transaminase (got) and glutamic-alanine transaminase (gpt). The presence of these enzymes has been established in human, bovine and rabbit seminal plasma (Flipse, 1960;Povoa & Villela, 1960;Gregoire, Rakoff & Ward, 1961), but no report on their presence in the epididymal fluid was found in the literature. Table 1 transamination activity in ram epididymal fluid, seminal The procedure of Reitman & Frankel (1957) for the determination of transaminases in blood serum was adapted to the epididymal fluid as follows: samples of epididymal semen were centrifuged with equal volumes of isotonic (0\m=.\15m) phosphate buffer at pH 7\m=.\3; the supernatant was diluted with 4 vol.…”

mentioning

confidence: 94%

“…Grégoire et al (1961) found that in normal human seminal plasma got activity was about twenty times greater than in blood serum, Povoa & Villela (1960) found it ten times greater, and Flipse (1960) found that the gpt activity in bovine seminal plasma was very weak as compared to the got activity, which was forty times greater.…”

mentioning

confidence: 95%

Transaminases in the Epididymal Fluid of the Ram

Alumot¹,

Schindler²

1965

Reproduction

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“…The occurrence of aspartate aminotransferase* and alanine aminotransferase in semen from man and certain mammals has been demonstrated by Povoa & Villela (1960), Flipse (1960), Grégoire, Rakoff 8c Ward (1961), Searcy, Craig & Bergquist (1962), Pumpianski & Sharon (1965, Joel & Herzberg (1965) and others. The aspartate aminotransferase activity in human semen was usually 10 to 20 times that in normal blood plasma.…”

Section: Introductionmentioning

confidence: 85%

“…There appeared to be a correlation between the enzyme activity and the sperm density both in human and bull semen. It has been suggested that part of the aspartate aminotrans¬ ferase activity could be due to a leakage of enzymes from the spermatozoa (Flipse, 1960).…”

Section: Introductionmentioning

confidence: 99%

Transaminase in Human Semen

Eliasson¹

1967

Reproduction

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Determinations of aspartate and alanine aminotransferase activity in human semen were carried out by the spectrophotometric and colorimetric methods. It was found that the occurrence of interfering substances in semen makes the spectrophotometric method unsuitable without prior partial purification of the enzyme. The mean activity determined colorimetrically in 417 samples of human semen was 320 Sigma-Frankel units/ml (s.e.m. \m=+-\8,range 10 to 1070) for aspartate aminotransferase and 30 units/ml (s.e.m. \ m=+-\ 3, range 0 to 97, n = 40) for alanine aminotransferase. As regards aspartate aminotransferase, the activity of this enzyme was highest at +60\s=deg\ C. Pyridoxal phosphate increased the activity, particularly if added to semen samples with initially low activity. Gel electrophoresis of seminal plasma showed one band migrating towards the anode. However, in homogenized spermatozoa the bulk of enzyme migrated towards the cathode and only a weak band could be demonstrated on the side of the anode.

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Metabolism of Bovine Semen. IX. Glutamic-Oxaloacetic and Glutamic-Pyruvic Transaminase Activities

Cited by 47 publications

References 10 publications

Studies of the Distribution and Source of Enzymes in Mammalian Semen

Studies of the Distribution and Source of Enzymes in Mammalian Semen

Transaminases in the Epididymal Fluid of the Ram

Transaminase in Human Semen

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