Metabolism of Cyclamate and Its Conversion to Cyclohexylamine

Collings, A.

doi:10.2337/diacare.12.1.50

Cited by 20 publications

(3 citation statements)

References 12 publications

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“…It is interesting to note that not all humans have the ability to convert cyclamate to cyclohexylamine. This process takes place in the gastrointestinal tract by intestinal flora [33]. In 1970 cyclamate was entirely outlawed by the FDA.…”

Section: Cyclamatementioning

confidence: 99%

Synthetic sweeteners and their impact on the gut microbiota - current state of knowledge

Karolina

Sebastian

Magda

2023

J Educ Health Sport

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Introduction and purpose: The aim of artificial sweeteners is to replace the sweetness of sugar without contributing to the higher energy consumption or unfavorable metabolic effects conventional sugar causes. Synthetic sweeteners - one of the most popular groups - are labeled as safe for use in foods and represented by acesulfame K, aspartame, neotame, saccharin, sucralose, cyclamate and advantame. Although they underwent safety procedures, their possible unbeneficial effects are still being evaluated. In this paper we focus on their impact on the gut microbiome. Brief description of the state of knowledge: For now, the advantage of search conducted on animals is observed. Those papers show the possibility of a relationship between artificial sweeteners and composition of the gut microbiota. Changes that were observed affected the gut microbiota in a negative way as they may be responsible for causing disbalance in the conglomerate of microorganisms. Studies on humans are limited. Long term consumption of acesulfame K and sucralose is linked with modulation of the composition of the microbiota. It has also been shown that synthetic sweeteners are able to increase the ability of bacteria to form a biofilm. Conclusions: Heretofore published paperworks about the impact of synthetic sweeteners on the gut microbiota are not sufficient to conduct valuable and unambiguous conclusions. Evidence collected until now is leading to a deduction that gut microbiota can be affected by the consumption of the sugar surrogates although more data is needed to clearly determine their possible role.

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Section: Cyclamatementioning

confidence: 99%

Synthetic sweeteners and their impact on the gut microbiota - current state of knowledge

Karolina

Sebastian

Magda

2023

J Educ Health Sport

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“…In the process of production and usage, CHAM is released into the atmosphere, water, and soil. Furthermore, It was found that sodium N-cyclohexylsulfamate, which is used as a food additive and produced 100,000 tons annually in China, could be decomposed into CHAM by intestinal bacteria ( Collings, 1989 ). CHAM is classified as a volatile organic compound that can enter human body via inhalation or skin contact.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp. YT-02

et al. 2018

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Cyclohexylamine (CHAM) is widely used in various industries, but it is harmful to human beings and the environment. Acinetobacter sp. YT-02 can degrade CHAM via cyclohexanone as an intermediate. In this study, the cyclohexylamine oxidase (CHAO) gene from Acinetobacter sp. YT-02 was cloned. Amino acid sequence alignment indicated that the cyclohexylamine oxidase (CHAOYT–02) was 48% identical to its homolog from Brevibacterium oxydans IH-35A (CHAOIH–35). The enzyme was expressed in Escherichia coli BL21 (DE3), and purified to apparent homogeneity by Ni-affinity chromatography. The purified enzyme was proposed to be a dimer of molecular mass of approximately 91 kDa. The enzyme exhibited its maximum activity at 50°C and at pH 7.0. The enzyme was thermolabile as demonstrated by loss of important percentage of its maximal activity after 30 min incubation at 50°C. Metal ions Mg2+, Co2+, and K+ had certain inhibitory effect on the enzyme activity. The kinetic parameters Km and Vmax were 0.25 ± 0.02 mM and 4.3 ± 0.083 μM min−1, respectively. The biochemical properties, substrate specificities, and three-dimensional structures of CHAOYT–02 and CHAOIH–35 were compared. Our results are helpful to elucidate the mechanism of microbial degradation of CHAM in the strain YT-02. In addition, CHAOYT–02, as a potential biocatalyst, is promising in controlling CHAM pollution and deracemization of chiral amines.

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“…Durante muitos anos o ciclamato foi considerado, sob vários aspectos, um aditivo ideal. Até então, acreditava se que sua absorção era lenta e parcial e que era eliminado de forma rápida e inalterada, com mínima ação tóxica (COLLINGS, 1991). Porém a segurança do ciclamato começou a ser questionada após observação em 1966, de que alguns indivíduos eram capazes de metabolizar ciclamato a ciclohexilamina e de que a ingestão crônica de misturas de ciclamato, sacarina e ciclohexilamina aumentava a incidência de tumores na bexiga de ratos (COLLINGS, 1991;FERNANDEZ, 1990).…”

Section: Ciclamato De Sódiounclassified

Avaliação dos níveis de ingestão diária de edulcorantes pelo consumo de adoçantes líquidos de mesa

Bianchi¹,

Michelle²

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Metabolism of Cyclamate and Its Conversion to Cyclohexylamine

Cited by 20 publications

References 12 publications

Synthetic sweeteners and their impact on the gut microbiota - current state of knowledge

Synthetic sweeteners and their impact on the gut microbiota - current state of knowledge

Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp. YT-02

Avaliação dos níveis de ingestão diária de edulcorantes pelo consumo de adoçantes líquidos de mesa

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