Metabolism of Fatty Acids by Ovine Spermatozoa

Neill, AR; Masters, C.J.

doi:10.1530/jrf.0.0340279

Cited by 45 publications

(18 citation statements)

References 12 publications

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“…Dott and Dingle (1968) detected DHA in ram sperm, whereas Scott et al (1967) did not report it as present in their extracts. Neill and Masters (1973) reported 65.1% DHA in total phospholipids of ovine sperm, consistent with the high proportion of DHA in ram sperm lipid in this study.…”

Section: Discussionsupporting

confidence: 90%

Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm

Samadian

Towhidi

Rezayazdi

et al. 2010

Animal

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The fatty acid composition of sperm affects the fertilization rate. The objective was to investigate the effects of dietary fish oil (as a source of n-3 fatty acids) on semen quality and sperm fatty acid composition in sheep. Eight Zandi fat-tailed rams were randomly allocated into two groups and fed either a control diet or a diet supplemented with fish oil. Both diets were isocaloric and isonitrogenous and were fed for 13 weeks, starting in the middle of the breeding season. Semen samples were collected weekly and their characteristics evaluated by standard methods, whereas samples collected at the start and end of the study were assessed (gas chromatography) for sperm lipid composition. Mean (6s.e.m.) sperm concentrations (4.3 3 10 9 6 1.3 3 10 8 v. 3.9 3 10 9 6 1.3 3 10 8 sperm/ml and percentages of motile (77.25 6 3.34 v. 60.8 6 3.34) and progressively motile sperm (74.13 6 1.69 v. 62.69 6 1.69) were significantly higher in the fish oil group than control. Dietary fish oil increased the proportion of docosahexaenoic acid (DHA, C22:6 n-3) in sperm fatty acid composition. We concluded that feeding fish oil as a source of n-3 fatty acids attenuated seasonal declines in semen quality in rams, perhaps through increased DHA in sperm.

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Section: Discussionsupporting

confidence: 90%

Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm

Samadian

Towhidi

Rezayazdi

et al. 2010

Animal

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“…By the early 1970s, the very high concentration in brain synaptosomal plasma membranes (6) and synaptic vesicles (7) was described by Breckenridge and co-workers. Table 1 presents the DHA composition in the aminophospholipids of brain and other selected mammalian tissues (1,(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). It is apparent that the DHA content of the nervous system is very high.…”

Section: Dha Compositionmentioning

confidence: 99%

Mechanisms of action of docosahexaenoic acid in the nervous system

Salem

Litman

Kim

et al. 2001

Lipids

825

508

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This review describes (from both the animal and human literature) the biological consequences of losses in nervous system docosahexaenoate (DHA). It then concentrates on biological mechanisms that may serve to explain changes in brain and retinal function. Brief consideration is given to actions of DHA as a nonesterified fatty acid and as a docosanoid or other bioactive molecule. The role of DHA-phospholipids in regulating G-protein signaling is presented in the context of studies with rhodopsin. It is clear that the visual pigment responds to the degree of unsaturation of the membrane lipids. At the cell biological level, DHA is shown to have a protective role in a cell culture model of apoptosis in relation to its effects in increasing cellular phosphatidylserine (PS); also, the loss of DHA leads to a loss in PS. Thus, through its effects on PS, DHA may play an important role in the regulation of cell signaling and in cell proliferation. Finally, progress has been made recently in nuclear magnetic resonance studies to delineate differences in molecular structure and order in biomembranes due to subtle changes in the degree of phospholipid unsaturation.Paper no. L8776 in Lipids 36, 945-959 (September 2001)

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“…DHA has been shown to be rapidly incorporated into various cells, primarily into phospholipids [46]. High concentration of DHA is found in retinal rod outer segment membranes [53], sperm [54], and synaptosomes [55], and didocosahexaenoyl phospholipid species have been isolated from several tissues [35][36][37]. In this experiment, diDHA did not affect Aβ polymerization, suggesting that free DHA, but not didocosahexaenoyl glycerol, exerted the inhibitory effect on Aβ 1-42 polymerization.…”

Section: Discussionmentioning

confidence: 79%

Evaluation of the inhibitory effect of docosahexaenoic acid and arachidonic acid on the initial stage of amyloid β1-42 polymerization by fluorescence correlation spectroscopy

Miwa¹,

Hashimoto²,

Hossain³

et al. 2013

AAD

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Amyloid β (Aβ) 1-42 fibrillation is a crucial step in the development of pathological hallmarks, such as neuritic plaques and neurofibrillary tangles, of Alzheimer's disease (AD). In this study, we evaluated the effects of free docosahexaenoic acid (DHA), an essential brain polyunsaturated fatty acid (PUFA), on the inhibition of Aβ 1-42 fibrillation by fluorescence correlation spectroscopy (FCS), a technique capable of detecting molecular movements and interactions in solution. We also examined whether free arachidonic acid (AA), eicosapentaenoic acid (EPA), and metabolites of DHA, including neuroprotectin D1 (NPD1, 10S, 17S-dihydroxy-DHA), resolvin D1 (RvD1, 7S, 8R, 17S-trihydroxy-DHA), and didocosahexaenoyl glycerol (diDHA), affect Aβ 1-42 polymerization. The results of the FCS study reveal that DHA and AA significantly reduced the diffusion time of TAMRA (5-carboxytetramethylrhodamine)-Aβ 1-42 by 28% and 31%, respectively, while EPA, NPD1, RvD1, and diDHA had no effects on diffusion time. These results indicate that DHA and AA inhibited Aβ 1-42 polymerization and that their inhibitory effects occurred at the initial stage of Aβ 1-42 polymerization. This study will advance the research on PUFAs in preventing AD progression.

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Metabolism of Fatty Acids by Ovine Spermatozoa

Cited by 45 publications

References 12 publications

Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm

Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm

Mechanisms of action of docosahexaenoic acid in the nervous system

Evaluation of the inhibitory effect of docosahexaenoic acid and arachidonic acid on the initial stage of amyloid β1-42 polymerization by fluorescence correlation spectroscopy

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