Metabolism of inorganic sulphur compounds byThiobacillus ferrooxidans and some comparative studies onThiobacillus A2 andT. neapolitanus

Kelly, Donovan P.; Tuovinen, Olli H.

doi:10.1007/bf01928477

Cited by 24 publications

(4 citation statements)

References 33 publications

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“…Trithionate was proposed to be reductively dismuted to thiosulphate and sulphite in T. thiooxidans (Okuzumi 1966^). Anaerobically, T. neapolitanus will decompose both trithionate and tetrathionate (Trudinger 1964 c, d;Kelly & Tuovinen 1975). Trudinger believed that the processes result from hydrolytic scission of either substrate to produce thiosulphate and sulphate, with sulphur or a hydrated sulphur compound of the form S(O H )2 from tetrathionate.…”

Section: A Common Pathway For Oxidation Of Sulphur Compoundsmentioning

confidence: 99%

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Biochemistry of the chemolithotrophic oxidation of inorganic sulphur

Kelly

1982

Phil. Trans. R. Soc. Lond. B

233

108

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A historical review is presented of the elucidation of the mechanisms of oxidation of inorganic sulphur compounds and of electron transport in the thiobacilli. A unitary mechanism, consistent with current knowledge, is proposed. The significance of polythionates is discussed. The relations between oxidation mechanisms, substrate-level and electron transport-dependent phosphorylation, energy-dependent NAD+ reduction and efficiency of growth are assessed in order to evaluate the efficiency of energy conservation in different species. The unresolved problems are identified for the benefit of those planning further assaults on the last redoubts of the thiobacilli.

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Section: A Common Pathway For Oxidation Of Sulphur Compoundsmentioning

confidence: 99%

“…An argument against an obligatory role for polythionates in a common oxidation mechanism is the total inability of some strains (e.g. Thiobacillus A2 (Kelly 1973;Kelly & Tuovinen 1975)) to produce or metabolize them. Trudinger (1964c?…”

Section: A Common Pathway For Oxidation Of Sulphur Compoundsmentioning

confidence: 99%

Biochemistry of the chemolithotrophic oxidation of inorganic sulphur

Kelly

1982

Phil. Trans. R. Soc. Lond. B

233

108

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“…thiopara had been grown only on sulphur, sulphide or thiosulphate (Caldwell et al, 1976;Brannan, 1981); T . neapolitanus grows on thiosulphate, although it is known also to use trithionate and tetrathionate (Trudinger, 1964;Kelly & Syrett, 1966); T. versutus grows on thiosulphate (Taylor & Hoare, 1969) but cannot metabolize polythionates (Kelly & Tuovinen, 1975); T. acidophilus and T. thiooxidans grow on elemental sulphur but while the latter has been shown to use thiosulphate, T . acidophilus was originally reported as unable to use soluble inorganic sulphur compounds (Guay & Silver, 1975), but was recently shown to use tetrathionate (Norris et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Chemolithotrophic and Autotrophic Growth of Thermothrix thiopara and Some Thiobacilli on Thiosulphate and Polythionates, and a Reassessment of the Growth Yields of Thx. thiopara in Chemostat Culture

1987

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Several thiobacilli and Thermothrix thiopara were grown in chemostat culture with inorganic sulphur compounds as growth-limiting energy substrates for autotrophic growth. Thiobacillus neapolitanus, Thiobacillus thiooxidans and Thiobacillus acidophilus were all able to use thiosulphate, trithionate or tetrathionate as sole energy substrates, as was Thx. thiopara grown at 72 "C. 'True growth yields' (Y,,,) were estimated for the organisms and showed yields of T . neapolitanus to be the same on trithionate and thiosulphate, thereby suggesting that only oxidative, and not substrate-level, phosphorylation is involved in energy conservation in this organism. The yield data suggested that substrate-level phosphorylation could, however, be significant in T. acidophilus. Thiobacillus versutus only grew with thiosulphate, with which it gave yield values similar to those of T . neapolitanus and T. thiooxidans. Thx. thiopara exhibited maximum specific growth rates on thiosulphate, trithionate and tetrathionate of 0.55,0.23-0.25 and <0-06 h-I respectively. Its yields on all three were much greater than those of the thiobacilli: the Y,,, on thiosulphate was in the range 18-0-22.8 g mol-I, but the organisms contained only about 29% carbon and 48% protein. These values do, however, make Thx. thiopara the highest-yielding inorganic-sulphur-oxidizing chemolithoautotroph yet described.

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“…Anaerobic tetrathionate metabolism was studied with Halothiobacillus neapolitanus [30] as well as Acidithiobacillus ferrooxidans [31]. In both cases, a stoichiometric ratio of 1:1 for degraded tetrathionate and produced thiosulphate resulted.…”

Section: Anaerobic Growth By Tetrathionate Disproportionationmentioning

confidence: 99%

Tetrathionate Disproportionation by Thiomonas intermedia K12

Wentzien

Sand²

2004

Engineering in Life Sciences

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Thiomonas intermedia K12, a moderately acidophilic bacterium, which oxidises sulphur compounds, ± exhibited the capability to use tetrathionate under oxic and anoxic conditions. Whereas under oxic conditions, the reduced sulphur tetrathionate compound was oxidised, under anoxic conditions, the organism disproportionated the compound. In both cases, trithionate and sulphate were produced but in different amounts. The results of the tetrathionate degradation experiments under oxic conditions pointed towards a cyclic degradation process with a transient formation of trithionate and sulphate as the final products, similar to the mechanism described for acidophilic sulphur compound oxidising bacteria. The results of the tetrathionate degradation experiments under anoxic conditions hinted to a partial reduction of tetrathionate to thiosulphate and a fractional oxidation to trithionate and sulphate. 4 M tetrathionate were converted to 6 M thiosulphate, 1 M trithionate, 1 M sulphate, and 8 M protons. The DG 0 ' of this reaction was found to be ±16.1 kJ per mol tetrathionate degraded. Additionally, Thiomonas intermedia K12 grew under anoxic conditions with tetrathionate as the sole energy source. The cell numbers increased from 10 5 as the start value to 10 7 /mL at the end. Organic compounds, excluding traces of yeast extract, did not enhance growth. Therefore, it is proposed that tetrathionate disproportionation is a novel lithotrophic metabolism, which allowed Thiomonas intermedia K12 to survive changing conditions of oxygen supply in sulphur-compound-rich environments and even to grow during this reaction. The extensive sulphur compound analysis was carried out by ion-pair chromatography.

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Metabolism of inorganic sulphur compounds byThiobacillus ferrooxidans and some comparative studies onThiobacillus A2 andT. neapolitanus

Cited by 24 publications

References 33 publications

Biochemistry of the chemolithotrophic oxidation of inorganic sulphur

Biochemistry of the chemolithotrophic oxidation of inorganic sulphur

Chemolithotrophic and Autotrophic Growth of Thermothrix thiopara and Some Thiobacilli on Thiosulphate and Polythionates, and a Reassessment of the Growth Yields of Thx. thiopara in Chemostat Culture

Tetrathionate Disproportionation by Thiomonas intermedia K12

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