1980
DOI: 10.1016/0304-4165(80)90289-5
|View full text |Cite
|
Sign up to set email alerts
|

Metabolism of sulfated glycosaminoglycans in rat hepatocytes synthesis of heparan sulfate and distribution into cellular and extracellular pools

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

2
11
0

Year Published

1981
1981
2006
2006

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 43 publications
(13 citation statements)
references
References 18 publications
2
11
0
Order By: Relevance
“…Earlier studies have shown that, when isolated rat hepatocytes are incubated with 35SO42-, most of the label is incorporated in heparan sulphate (Prinz et al, 1980;Ninomiya et al, 1981). In agreement with those studies, we found that when samples of detergent-solubilized cells were analysed by gel electrophoresis, most of the radioactive material hardly entered the separating gel (Marcks von Wurtemberg & Fries, 1989), a property characteristic of the heparan sulphate proteoglycan of hepatocytes.…”
Section: Discussionsupporting
confidence: 91%
“…Earlier studies have shown that, when isolated rat hepatocytes are incubated with 35SO42-, most of the label is incorporated in heparan sulphate (Prinz et al, 1980;Ninomiya et al, 1981). In agreement with those studies, we found that when samples of detergent-solubilized cells were analysed by gel electrophoresis, most of the radioactive material hardly entered the separating gel (Marcks von Wurtemberg & Fries, 1989), a property characteristic of the heparan sulphate proteoglycan of hepatocytes.…”
Section: Discussionsupporting
confidence: 91%
“…Thus, it appears that the xyloside-linked 35 S-GAGs in this system are composed mainly of heparan sulfate (70%) with some chondroitin sulfate (20%). These results are consistent with the finding that the major GAG synthesized by liver is heparan sulfate (48). Using p-nitrophenyl-␤-D-xyloside instead of 4-methylumbelliferyl-␤-D-xyloside as external acceptor gave similar results suggesting that it is the xylosyl group and not the aglycone moiety that drives the initiation for GAG synthesis.…”
Section: In Vitro Synthesis Of Gags By Rat Liver Golgi-incubation Of supporting
confidence: 81%
“…Previous papers report that HS is distributed throughout the extracellular, cell surface, and intracellular regions of cultured cells, and that the culture medium of cells will often contain a mixture of HS complexed into proteoglycans as well as liberated, free‐floating HS chains (Prinz et al, 1980; Robinson et al, 1984). Cells have previously been shown to contain several structurally different species of HS harvested from the different compartments.…”
Section: Discussionmentioning
confidence: 99%