Metabolite profile changes in xylem sap and leaf extracts of strategy I plants in response to iron deficiency and resupply

Rellán‐Álvarez, Rubén; El-Jendoubi, Hamdi; Wohlgemuth, Gert; Abadı́a, Anunciación; Fiehn, Oliver; Abadı́a, Javier; Álvarez‐Fernández, Ana

doi:10.3389/fpls.2011.00066

Cited by 45 publications

(50 citation statements)

References 69 publications

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“…Metabolite extraction was carried out as previously described for leaf extracts (Fiehn and Weckwerth, 2003; Fiehn et al , 2008; Rellán-Álvarez et al , 2011). Dried extracts were derivatized as described elsewhere (Fiehn et al , 2008).…”

Section: Methodsmentioning

confidence: 99%

Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an Arabidopsis ferritin mutant

Sudre¹,

Gutiérrez-Carbonell²,

Lattanzio³

et al. 2013

Journal of Experimental Botany

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Iron homeostasis is an important process for flower development and plant fertility. The role of plastids in these processes has been shown to be essential. To document the relationships between plastid iron homeostasis and flower biology further, a global study (transcriptome, proteome, metabolome, and hormone analysis) was performed of Arabidopsis flowers from wild-type and triple atfer1-3-4 ferritin mutant plants grown under iron-sufficient or excess conditions. Some major modifications in specific functional categories were consistently observed at these three omic levels, although no significant overlaps of specific transcripts and proteins were detected. These modifications concerned redox reactions and oxidative stress, as well as amino acid and protein catabolism, this latter point being exemplified by an almost 10-fold increase in urea concentration of atfer1-3-4 flowers from plants grown under iron excess conditions. The mutant background caused alterations in Fe–haem redox proteins located in membranes and in hormone-responsive proteins. Specific effects of excess Fe in the mutant included further changes in these categories, supporting the idea that the mutant is facing a more intense Fe/redox stress than the wild type. The mutation and/or excess Fe had a strong impact at the membrane level, as denoted by the changes in the transporter and lipid metabolism categories. In spite of the large number of genes and proteins responsive to hormones found to be regulated in this study, changes in the hormonal balance were restricted to cytokinins, especially in the mutant plants grown under Fe excess conditions.

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Section: Methodsmentioning

confidence: 99%

Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an Arabidopsis ferritin mutant

Sudre¹,

Gutiérrez-Carbonell²,

Lattanzio³

et al. 2013

Journal of Experimental Botany

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show abstract

“…In addition to the increased accumulation of organic acids, particularly citric acid, in roots facilitating Fe mobilization (Rellán-Álvarez et al, 2011;Valentinuzzi et al, 2015), metabolite profiles reported in Figure 8 and Table II showed that Fe deficiency induced an accumulation of Glc and Fru. A drop of pyruvic acid in plants exposed to sulfate deficiency indicates a slowdown of the glycolytic pathway, especially prevalent under combined Fe and S starvation, where additionally Glc-6-P levels were reduced (Fig.…”

Section: Is There a Differential Metabolic Response To Fe Deficiency mentioning

confidence: 97%

The interplay between sulfur and iron nutrition in tomato

et al. 2015

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“…Although Fe is one of the most abundant elements in the earth’s crust, its bioavailability is severely restricted due to an extremely low solubility at neutral or basic pH. Approximately 30% of the cultivated plants are grown on calcareous soils, making Fe deficiency a major constraint for crop yield and quality (Rellan-Alvarez et al, 2011). Excess Fe is cytotoxic due to the formation of potentially harmful reactive oxygen species.…”

Section: Introductionmentioning

confidence: 99%

A Digital Compendium of Genes Mediating the Reversible Phosphorylation of Proteins in Fe-Deficient Arabidopsis Roots

Lan

Schmidt

2013

Front. Plant Sci.

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Post-translational modifications of proteins such as reversible phosphorylation provide an important but understudied regulatory network that controls important nodes in the adaptation of plants to environmental conditions. Iron (Fe) is an essential mineral nutrient for plants, but due to its low solubility often a limiting factor for optimal growth. To understand the role of protein phosphorylation in the regulation of cellular Fe homeostasis, we analyzed the expression of protein kinases (PKs) and phosphatases (PPs) in Arabidopsis roots by mining differentially expressed PK and PP genes. Transcriptome analysis using RNA-seq revealed that subsets of 203 PK and 39 PP genes were differentially expressed under Fe-deficient conditions. Functional modules of these PK and PP genes were further generated based on co-expression analysis using the MACCU toolbox on the basis of 300 publicly available root-related microarray data sets. Results revealed networks comprising 87 known or annotated PK and PP genes that could be subdivided into one large and several smaller highly co-expressed gene modules. The largest module was composed of 58 genes, most of which have been assigned to the leucine-rich repeat protein kinase superfamily and associated with the biological processes “hypotonic salinity response,” “potassium ion import,” and “cellular potassium ion homeostasis.” The comprehensive transcriptional information on PK and PP genes in iron-deficient roots provided here sets the stage for follow-up experiments and contributes to our understanding of the post-translational regulation of Fe deficiency and potassium ion homeostasis.

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Metabolite profile changes in xylem sap and leaf extracts of strategy I plants in response to iron deficiency and resupply

Cited by 45 publications

References 69 publications

Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an Arabidopsis ferritin mutant

Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an Arabidopsis ferritin mutant

The interplay between sulfur and iron nutrition in tomato

A Digital Compendium of Genes Mediating the Reversible Phosphorylation of Proteins in Fe-Deficient Arabidopsis Roots

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