Metabolite Profiles in Starved Diporeia spp. Using Liquid Chromatography-Mass Spectrometry (LC-MS) Based Metabolomics

Maity, Suman Kumar; Jannasch, Amber; Adamec, Jiřı́; Gribskov, Michael; Nalepa, Thomas F.; Höök, Tomas O.; Sepúlveda, Marı́a S.

doi:10.1163/193724011x615578

Cited by 23 publications

(28 citation statements)

References 83 publications

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“…Zooplankton are DHA-limited in the detritivorous pathway throughout the parameter domain (Figure 3A). Recent work has shown that a range of aquatic invertebrates, including marine zooplankton, catabolize essential PUFAs at high rates (Mezek et al, 2010; Mayor et al, 2011, 2015; Maity et al, 2012) in which case our default zooplankton NPE for DHA of 0.9 (Anderson and Pond, 2000; Mayor et al, 2009) may be too high. Reducing the value of this parameter results in a proportional lowering of predicted zooplankton growth, to the extent that the detritivorous pathway becomes an inferior source of nutrition relative to the microbial pathway (in areas of the plane shown in Figure 3A that are lower than those of the corresponding parameter space shown in Figure 3B).…”

Section: Resultsmentioning

confidence: 94%

The Role of Microbes in the Nutrition of Detritivorous Invertebrates: A Stoichiometric Analysis

2017

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Detritus represents an important pool in the global carbon cycle, providing a food source for detritivorous invertebrates that are conspicuous components of almost all ecosystems. Our knowledge of how these organisms meet their nutritional demands on a diet that is typically comprised of refractory, carbon-rich compounds nevertheless remains incomplete. “Trophic upgrading” of detritus by the attached microbial community (enhancement of zooplankton diet by the inclusion of heterotrophic protozoans) represents a potential source of nutrition for detritivores as both bacteria and their flagellated protistan predators are capable of biosynthesizing essential micronutrients such as polyunsaturated fatty acids (PUFAs). There is however a trade-off because although microbes enhance the substrate in terms of its micronutrient content, the quantity of organic carbon is diminished though metabolic losses as energy passes through the microbial food web. Here, we develop a simple stoichiometric model to examine this trade-off in the nutrition of detritivorous copepods inhabiting the mesopelagic zone of the ocean, focusing on their requirements for carbon and an essential PUFA, docosahexaenoic acid (DHA). Results indicate that feeding on microbes may be a highly favorable strategy for these invertebrates, although the potential for carbon to become limiting when consuming a microbial diet exists because of the inefficiencies of trophic transfer within the microbial food web. Our study highlights the need for improved knowledge at the detritus-microbe-metazoan interface, including interactions between the physiology and ecology of the associated organisms.

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Section: Resultsmentioning

confidence: 94%

The Role of Microbes in the Nutrition of Detritivorous Invertebrates: A Stoichiometric Analysis

2017

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“…Increasing in LysoPC could be connected with the lower nutritional value of M. subterranean 848/1 and the attempt to produce energy by polar lipid catabolism in the juvenile mussels. The catabolism of phospholipids was also observed on starved amphipods [97] and on crab larvae approaching metamorphosis [98].…”

Section: Discussionmentioning

confidence: 99%

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulisL. 1758), a key economic and ecological species

Laudicella

Beveridge

Carboni

et al. 2019

Preprint

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Blue mussels (Mytilus edulis L.) are important components of coastal ecosystems functioning through benthopelagic coupling and ecosystem engineering. At the same time, mussel production is central in the economy of coastal areas. Therefore, understanding their nutritional, physiological and metabolic processes at key life stages is important for their management, both within food production systems and in wild populations.Lipids are crucial molecules for bivalve growth, but their diversity and roles have been considered from fatty acid (FA) perspective. In this paper, we applied lipidomics to bivalve nutrition. Lipidomics provides a holistic perspective on lipid patterns; by examining the lipidome, important physiological information can be acquired. Here, we use controlled laboratory experiments to elucidate the responses to changes in the diet of newly settled mussels juveniles, one of the most critical life stages. The diets considered in this study are single strains diet of Cylindrotheca fusiformis CCAP 1017/2 – CYL, Isochrysis galbana CCAP 927/1– ISO, Monodopsis subterranean CCAP 848/1 – MONO, Nannochloropsis oceanica CCAP 849/10– NANNO and a commercial algae paste –SP.The diets had a significant effect on spat GR and WI, and according to their efficacy resulted ranked as follows: ISO>NANNO/CYL>SP>MONO. Spat FA composition and neutral lipid content (principally triacylglycerols - TG), were influenced by the diets. Furthermore, untargeted lipidomics also showed shifts in several phospholipid species, with changes related to the essential PUFA available from the diet. TG content, neutral lipids and several TG and FA species were correlated (Spearman R2>0.8 FDR p<0.05) with spat WI, suggesting their possible application as markers of mussel juvenile condition. The availability of dietary essential PUFA deeply modified the spat lipidome both for neutral and for polar lipids. This change in the lipidome could have major impacts on their ecology and their production for food.

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“…We previously reported the presence of 1-I2MU in Diporeia that were starved for 60 d (Maity et al 2012b). 1-I2MU levels ranged widely across individuals and decreased during our starvation trials.…”

Section: Diatom Feeding Experimentsmentioning

confidence: 95%

“…Metabolomics data from whole organisms were collected using a combination of mass spectrometry coupled with two-dimensional gas chromatography (GCXGC) and liquid chromatography (LC) following protocols described by Maity et al (2012aMaity et al ( , 2012b and Ralston-Hooper et al (2008) with some modifications. Briefly, each sample (composed of a single Diporeia, 0.0021-0.0098 g) was homogenized, methanol (300 mL) and chloroform (450 mL) were added, and the sample was centrifuged in order to separate polar (methanol : water) and nonpolar (chloroform) phases.…”

Section: Metabolomic Analysesmentioning

confidence: 99%

“…This induced scarcity of food could result in compromised survival and reproductive output by Diporeia, as it did in other amphipod species (Sundelin et al 2008). Previously, impacts of such starvation on the physiology of Diporeia have been investigated in laboratory-based mesocosm studies using metabolomics and RNA : DNA ratios, which found disrupted protein and lipid metabolism and declining RNA : DNA ratios under stress (Ryan 2010;Maity et al 2012a).…”

Section: Introductionmentioning

confidence: 99%

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Elucidating Causes ofDiporeiaDecline in the Great Lakes via Metabolomics: Physiological Responses after Exposure to Different Stressors

Maity¹,

Jannasch²,

Adamec³

et al. 2013

Physiological and Biochemical Zoology

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The benthic macroinvertebrate Diporeia spp. have been extirpated from many areas of the Laurentian Great Lakes, but the mechanisms underlying such declines are not fully understood. Diporeia declines coinciding with the invasion of exotic dreissenid mussels (zebra and quagga) have led to the hypothesis that Diporeia declines are a result of decreased food availability from increasing competition with dreissenids for diatoms. There is additional evidence that Diporeia are negatively affected when in close proximity to dreissenids, probably because of exposure to toxins present in the mussels' pseudofeces. Diporeia are also known to be sensitive to anthropogenic contaminants (such as polychlorinated biphenyls [PCBs]) present in Great Lakes sediments. To better understand the physiological responses of Diporeia to diverse stressors, we conducted three 28-d experiments evaluating changes in the metabolomes of Diporeia (1) fed diatoms (Cyclotella meneghiniana) versus starved, (2) exposed (from Lake Michigan and Cayuga Lake) to quagga mussels (Dreissena bugensis), and (3) exposed to sediments contaminated with PCBs. The metabolomes of samples were examined using both two-dimensional gas and liquid chromatography coupled with mass spectrometry. Each stressor elicited a unique metabolome response characterized by enhanced citric acid cycle, fatty acid biosynthesis, and protein metabolism in diatom-fed Diporeia; impaired glycolysis, protein catabolism, and folate metabolism in Diporeia from Lake Michigan irrespective of quagga mussel exposure, suggesting lake-specific adaptation mechanisms; and altered cysteine and phospholipid metabolism during PCB exposure. Subsequent comparisons of these stressor-specific metabolic responses with metabolomes of a feral Diporeia population would help identify stressors affecting Diporeia populations throughout the Great Lakes.

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Metabolite Profiles in Starved Diporeia spp. Using Liquid Chromatography-Mass Spectrometry (LC-MS) Based Metabolomics

Cited by 23 publications

References 83 publications

The Role of Microbes in the Nutrition of Detritivorous Invertebrates: A Stoichiometric Analysis

The Role of Microbes in the Nutrition of Detritivorous Invertebrates: A Stoichiometric Analysis

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulisL. 1758), a key economic and ecological species

Elucidating Causes ofDiporeiaDecline in the Great Lakes via Metabolomics: Physiological Responses after Exposure to Different Stressors

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