Metabolite Profiling: A Tool for the Biochemical Characterisation of Mycobacterium sp.

Drapal, Margit; Fraser, Paul D.

doi:10.3390/microorganisms7050148

Cited by 7 publications

(4 citation statements)

References 72 publications

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“…This enabled the identification of various host-associated metabolic pathways linked to the progression of TB in the mouse model [ 149 ]. Metabolomics can be further applied to studies of mechanisms of actions of previously uncharacterized antimicrobial compounds [ 150 ], characterization of vaccine candidates [ 151 ] and have potential in drug discovery programs of Mtb [ 152 ].…”

Section: Systems Biology Methodology and Novel Applications In mentioning

confidence: 99%

Metabolic Versatility of Mycobacterium tuberculosis during Infection and Dormancy

Chang

Guan

2021

Metabolites

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Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), is a highly successful intracellular pathogen with the ability to withstand harsh conditions and reside long-term within its host. In the dormant and persistent states, the bacterium tunes its metabolism and is able to resist the actions of antibiotics. One of the main strategies Mtb adopts is through its metabolic versatility—it is able to cometabolize a variety of essential nutrients and direct these nutrients simultaneously to multiple metabolic pathways to facilitate the infection of the host. Mtb further undergo extensive remodeling of its metabolic pathways in response to stress and dormancy. In recent years, advancement in systems biology and its applications have contributed substantially to a more coherent view on the intricate metabolic networks of Mtb. With a more refined appreciation of the roles of metabolism in mycobacterial infection and drug resistance, and the success of drugs targeting metabolism, there is growing interest in further development of anti-TB therapies that target metabolism, including lipid metabolism and oxidative phosphorylation. Here, we will review current knowledge revolving around the versatility of Mtb in remodeling its metabolism during infection and dormancy, with a focus on central carbon metabolism and lipid metabolism.

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Section: Systems Biology Methodology and Novel Applications In mentioning

confidence: 99%

Metabolic Versatility of Mycobacterium tuberculosis during Infection and Dormancy

Chang

Guan

2021

Metabolites

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“…Compared to other bacteria, the growth of most mycobacterial species was slow, up to 20 hours on commonly used media. A natural division existed between slow-growing and fast-growing mycobacterial species (Drapal & Fraser 2019). Slow-growing mycobacteria required more than seven days to colonize on solid media from aqueous inoculum under ideal culture conditions.…”

Section: Resultsmentioning

confidence: 99%

Castor Plant (Ricinus communis L.) Leaf Extract as Potential Antibacterial Against The Growth of Mycobacterium Tuberculosis

Istaufa

Subagio

Suswati

et al. 2022

FMI

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Highlight : Patients experiencing failure of first-line drug and developing multidrug resistant tuberculosis (MDR-TB) has increased throughout 2011-2015. Castor plant leaf extract (Ricinus communis L.) has an antibacterial potential against the growth of Mycobacterium tuberculosis bacteria. Abstract: Mycobacterium tuberculosis is the cause of pulmonary tuberculosis that can reduce human health. In the therapy of the disease, patients can develop resistance to tuberculosis drugs. Based on the 2015 health profiles of Indonesia, 15,380 people were suspected to have multidrug-resistant tuberculosis (MDR-TB), while 1,860 people were confirmed patients with MDR-TB. There is a need for innovation to develop the latest treatments using natural ingredients, one of which is castor plant (Ricinus communis L.) that contains antibacterial compounds against Mycobacterium tuberculosis. This study aimed to understand the antimicrobial potential of castor plant (Ricinus communis L.) leaf extract against the growth of Mycobacterium tuberculosis. This scientific paper was a quantitative systematic review study. Literature in the form of journal articles and books were obtained through search engines, i.e. ebook database, Google Scholar, Cochrane, Wiley, and PubMed. The results of the literature source search were 19 journal articles and 4 ebooks, as well as 4 journal articles that were in accordance with the title of this literature review and discussed the effects of castor plants on the growth of Mycobacterium tuberculosis. The results of the analysis showed that castor plant (Ricinus communis L.) leaf extract has the potential in the antibacterial activity against the growth of Mycobacterium tuberculosis because it contains phytochemicals in the form of flavonoids, saponins, alkaloids, tannins, and fatty acid amides derived from ricinoleic acid as the main constituent of castor plants (Ricinus communis L.). There is antimicrobial potential for castor plant (Ricinus communis L.) leaf extract against the growth of Mycobacterium tuberculosis.

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“…This approach has led to the discovery of the biochemical functions of many essential enzymes in Mtb, including enzymes in central carbon metabolism and lipid and amino acid biosynthesis. 88 Conversely, the dynamic change and intracellular levels of small molecules can be mapped to a genotype as well. Understanding the changes in the levels of these biological molecules has been exploited as a useful tool in the identification of antibiotic function and more specifically in validating the function of an metabolic enzyme target.…”

Section: Metabolic Adaptations To Antibiotic Perturbations Can Be Studiedmentioning

confidence: 99%

The pursuit of mechanism of action: uncovering drug complexity in TB drug discovery

2021

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Metabolite Profiling: A Tool for the Biochemical Characterisation of Mycobacterium sp.

Cited by 7 publications

References 72 publications

Metabolic Versatility of Mycobacterium tuberculosis during Infection and Dormancy

Metabolic Versatility of Mycobacterium tuberculosis during Infection and Dormancy

Castor Plant (Ricinus communis L.) Leaf Extract as Potential Antibacterial Against The Growth of Mycobacterium Tuberculosis

The pursuit of mechanism of action: uncovering drug complexity in TB drug discovery

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