Metabolites from Microalgae Schizochytrium mangrovei

Hang, Nguyen Thi Minh; Giap, Tran Huu; Le, Thanh Nguyen; Oanh, Nguyen Thi Tu; Hồng, Đặng Diễm; Minh, Châu Văn

doi:10.1007/s10600-019-02868-4

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…The ALA, LA, EPA, and DHA polyunsaturated fatty acids were extracted and purified as described by Hang et al [76]. The structures of purified compounds were confirmed with nuclear magnetic resonance (NMR) spectroscopy.…”

Section: Extraction and Purification Of Ala La Epa And Dhamentioning

confidence: 99%

Characterization and Optimization of Culture Conditions for Aurantiochytrium sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture

Hien

Thom

et al. 2022

Marine Drugs

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Aurantiochytrium is a heterotrophic marine microalga that has potential industrial applications. The main objectives of this study were to isolate an Aurantiochytrium strain from Sand Cay (Son Ca) Island, Vietnam, optimize its culture conditions, determine its nutritional composition, extract polyunsaturated fatty acids (PUFAs) in the free (FFA) and the alkyl ester (FAAE) forms, and evaluate the antioxidation and neuroprotection properties of the PUFAs. Aurantiochytrium sp. SC145 can be grown stably under laboratory conditions. Its culture conditions were optimized for a dry cell weight (DCW) of 31.18 g/L, with total lipids comprising 25.29%, proteins 7.93%, carbohydrates 15.21%, and carotenoid at 143.67 µg/L of DCW. The FAAEs and FFAs extracted from Aurantiochytrium sp. SC145 were rich in omega 3–6–9 fatty acids (40.73% and 44.00% of total fatty acids, respectively). No acute or subchronic oral toxicity was determined in mice fed with the PUFAs in FFA or FAAE forms at different doses over 90 days. Furthermore, the PUFAs in the FFA or FAAE forms and their main constituents of EPA, DHA, and ALA showed antioxidant and AChE inhibitory properties and neuroprotective activities against damage caused by H2O2- and amyloid-ß protein fragment 25–35 (Aβ25-35)-induced C6 cells. These data suggest that PUFAs extracted from Aurantiochytrium sp. SC145 may be a potential therapeutic target for the treatment of neurodegenerative disorders.

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Section: Extraction and Purification Of Ala La Epa And Dhamentioning

confidence: 99%

Characterization and Optimization of Culture Conditions for Aurantiochytrium sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture

Hien

Thom

et al. 2022

Marine Drugs

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“…HX-308 ( Ren et al, 2015 ), Schizochytrium limacinum B4D1 ( Zhu et al, 2022 ), Schizochytrium sp. S31 ( Jiang et al, 2020 ), Schizochytrium mangrovei PQ6 ( Hang et al, 2019 ), and Schizochytrium sp. MYA1381 ( Li et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Elucidation of sterol biosynthesis pathway and its co-regulation with fatty acid biosynthesis in the oleaginous marine protist Schizochytrium sp.

Bi¹,

Guo²,

Liu³

et al. 2023

Front. Bioeng. Biotechnol.

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Sterols constitute vital structural and regulatory components of eukaryotic cells. In the oleaginous microorganism Schizochytrium sp. S31, the sterol biosynthetic pathway primarily produces cholesterol, stigmasterol, lanosterol, and cycloartenol. However, the sterol biosynthesis pathway and its functional roles in Schizochytrium remain unidentified. Through Schizochytrium genomic data mining and a chemical biology approach, we first in silico elucidated the mevalonate and sterol biosynthesis pathways of Schizochytrium. The results showed that owing to the lack of plastids in Schizochytrium, it is likely to use the mevalonate pathway as the terpenoid backbone pathway to supply isopentenyl diphosphate for the synthesis of sterols, similar to that in fungi and animals. In addition, our analysis revealed a chimeric organization of the Schizochytrium sterol biosynthesis pathway, which possesses features of both algae and animal pathways. Temporal tracking of sterol profiles reveals that sterols play important roles in Schizochytrium growth, carotenoid synthesis, and fatty acid synthesis. Furthermore, the dynamics of fatty acid and transcription levels of genes involved in fatty acid upon chemical inhibitor-induced sterol inhibition reveal possible co-regulation of sterol synthesis and fatty acid synthesis, as the inhibition of sterol synthesis could promote the accumulation of fatty acid in Schizochytrium. Sterol and carotenoid metabolisms are also found possibly co-regulated, as the inhibition of sterols led to decreased carotenoid synthesis through down-regulating the gene HMGR and crtIBY in Schizochytrium. Together, elucidation of the Schizochytrium sterol biosynthesis pathway and its co-regulation with fatty acid synthesis lay the essential foundation for engineering Schizochytrium for the sustainable production of lipids and high-value chemicals.

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“…In addition to PUFAs and squalene exploitation (Dang Diem Hong & Hoang Thi Lan Anh, 2016), the study of Hang et al (2019) is the first publication on bioactive compounds extracted from Schizochytrium mangrovei PQ6 including ten compounds as 4α-methyl-5α-ergosta-7,22-dien-3β-ol…”

Section: Introductionmentioning

confidence: 99%

Optimization of cultivation conditions of the heterotrophic marine microalga \(\textit{Thraustochytrium aureum}\) BT6 oriented to exploit bioactive compounds

Thom

Hien

Thu

et al. 2021

AJB

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Thraustochytrium is a heterotrophic marine microalgae genus belonging to the Labyrinthula class, Thraustochytriaceae (Thraustochytrids) family. Recently, studied results have showed that some species/strains of Thraustochytrium genus are potential candidates to produce biomass rich in lipid containing high polyunsaturated fatty acid (PUFAs), especially docosahexaenoic (DHA C22: 6 ω-3), carotenoid, extracellular polysaccharides (EPSs) and enzymes which play a crucial role in human health. Therefore, throughout many decades, numerous studies have been conducted in exploiting bioactive compounds, especially PUFAs. Biomass and PUFAs yield of some strains Thraustochytrium spp. depend on environmental conditions. In this paper, the results on the effects of different culture conditions on the growth of Thraustochytrium aureum BT6 (isolated from coastal regions of Binh Thuan in 2010) in 500 mL erlenmeyer flask and 30 L fermentors is presented. Suitable conditions for the growth of strain BT6 were: Bajpai medium, 2% glucose, 0.5% yeast extract, temperature of 20−28 oC, salt concentration 0.5%; and initial algal cell concentration of 2 g/L. The dry biomass, lipid and DHA contents of strain BT6 in 500 mL erlenmeyer flasks and 30 L fermentor reached the highest levels of 8.56 ± 0.12 and 6.92 ± 0.27 g/L, 18.36 ± 1.15 and 5.50 ± 0.133% DCW, 2.00 ± 0.05 and 0.14 ± 0.01% DCW, respectively, after 4 days of cultivation. Carotenoid content in flask and 30L fermentor achieved 44.45 ± 1.4 and 28.84 ± 1.12 mg/kg DCW, respectively after 4 days of cultivation. Obtained results have indicated that the algae biomass met the requirements in orientation to exploit valuable bioactive compounds.

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Metabolites from Microalgae Schizochytrium mangrovei

Cited by 4 publications

References 9 publications

Characterization and Optimization of Culture Conditions for Aurantiochytrium sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture

Characterization and Optimization of Culture Conditions for Aurantiochytrium sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture

Elucidation of sterol biosynthesis pathway and its co-regulation with fatty acid biosynthesis in the oleaginous marine protist Schizochytrium sp.

Optimization of cultivation conditions of the heterotrophic marine microalga \(\textit{Thraustochytrium aureum}\) BT6 oriented to exploit bioactive compounds

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