Metabolites from the endophytic fungus Cylindrocarpon sp. isolated from tropical plant Sapium ellipticum

Kamdem, Ramsay S.T.; Wafo, Pascal; Rehberg, Nidja; Geelen, Lasse van; Höfert, Simon-Patrick; Knedel, Tim‐Oliver; Janiak, Christoph; Sureechatchaiyan, Parichat; Kassack, Matthias U.; Lin, Wenhan; Kalscheuer, Rainer; Liu, Zhen; Proksch, Peter

doi:10.1016/j.fitote.2018.05.020

Cited by 14 publications

(11 citation statements)

References 20 publications

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“…In our research, ethyl acetate extracts of 15 endophytic fungi (15% of total screened) showed inhibitory activity against pathogenic microorganisms. These 15 isolates represent fungal genera previously reported to produce antimicrobial compounds (Ouyang et al, 2018;Kamdem et al, 2018;Yu et al, 2018;Deshmukh, Verekar & Bhave, 2014). However, new bioactive compounds are constantly being discovered from plant endophytes (Rateb et al, 2011;Sayed et al, 2019;Chen et al, 2019a).…”

Section: Antimicrobial Activity Screening Of the Ethyl Acetate Extracts From Endophytic Fungal Culture Filtratesmentioning

confidence: 99%

Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

An¹,

Ma²,

Shi³

et al. 2020

PeerJ

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Endophytic fungi have been emerged as fruitful resources for producing structurally fascinating and biologically active secondary metabolites. However, endophytic fungi from medicinal plants of Qinling Mountains–the most important natural climatic boundary between the subtropical and warm temperate zones of China with an astonishingly high level of biodiversity–have rarely been explored as potential sources of novel fungal species and active secondary metabolites. In this study, a total of 371 fungal colonies were successfully isolated from 510 tissue segments of the medicinal Tupistra chinensis Baker collected from Qinling Mountains, China. Roots of T. chinensis Baker are used as a folk medicine to ameliorate pharyngitis and treat rheumatic diseases. A total of 100 representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), seven classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Microbotryomycetes, Agaricomycetes, Leotiomycetes, Mortierellomycetes), and at least 35 genera. The genera of Collectotrichum (IF, 29.92%), Fusarium (IF, 8.36%), Aspergillus (IF, 8.09%), and Dactylonectria (IF, 5.39%) were most frequently isolated from the tissues of T. chinensis Baker. The Species Richness Index (S, 65) and the Shannon-Wiener Index (H′, 3.7914) indicated that T. chinensis Baker harbored abundant fungal resources. Moreover, five isolates were potential new taxa because of low similarity of ITS sequences ranged from 95.09%∼96.61%. Fifteen out of 100 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Two important lead compounds produced by two stains (F8047 and F8075) with high antimicrobial activities were identified using high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC–QTOF MS) analyses. In addition, it was noteworthy that the strain F8001, which may be a potential new species, showed antimicrobial activity and should be investigated further. Overall, these results indicated that the endophytic fungi from T. chinensis Baker could be exploited as a novel source of bioactive compounds.

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Section: Antimicrobial Activity Screening Of the Ethyl Acetate Extracts From Endophytic Fungal Culture Filtratesmentioning

confidence: 99%

Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

An¹,

Ma²,

Shi³

et al. 2020

PeerJ

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“…The structures of 135-139 were established as (2R)-6-hydroxy-2,4-dimethoxy-5-methylphthalide ( 135), 4,6-dihydroxy-5-hydroxymethylphthalide9 ( 136), 4,6-dihydroxy-5-methoxymethylphthalide ( 137), (2R)-4,5dihydroxy-2,6-dimethoxy-2-pentylphthalide ( 138), (E)-4,5-dihydroxy-2-(4-hydroxypentylidene)-6-methoxyphthalide (139), respectively [45]. Three new polyketides, cylindrocarpones A-C 147-149 (Figure 5), were isolated from the endophytic fungus, Cylindrocarpon sp., obtained from the tropical plant Sapium ellipticum [46]. Six new xanthone-derived polyketides, named phomoxanthones F-K 150-155 (Figure 5), were isolated from Phomopsis sp.…”

Section: Ketone Compounds 221 Polyketidesmentioning

confidence: 99%

“…ASCLA isolated from leaf tissues of the medicinal plant Callistemon subulatus [68]. From the endophytic fungus, Cylindrocarpon sp., obtained from the tropical plant Sapium ellipticum, a new pyrone cylindropyrone 213 (Figure 6) was isolated [46]. One new benzophenone derivative, named tenllone I 214 (Figure 6), was isolated from the endophytic fungus Diaporthe lithocarpus A740 [41].…”

Section: Other Ketonesmentioning

confidence: 99%

“…A new alkaloid, 1, 2-dihydrophenopyrrozin 218 (Figure 7), was isolated from an axenic culture of the endophytic fungus, Bionectria sp., obtained from seeds of the tropical plant Raphia taedigera [70]. Two new pyridone alkaloids, cylindrocarpyridones A−B 219-220 (Figure 7), were isolated from the endophytic fungus, Cylindrocarpon sp., obtained from the tropical plant Sapium ellipticum [46]. From Aspergillus versicolor, an endophyte derived from leaves of the Egyptian water hyacinth Eichhornia crassipes (Pontederiaceae), one new compound aflaquinolone H 221 (Figure 7) belonging to dihydroquinolone alkaoids was obtained [71].…”

Section: Alkaloids and Their Derivativesmentioning

confidence: 99%

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Biological Activities of Some New Secondary Metabolites Isolated from Endophytic Fungi: A Review Study

Zheng

Zhang

et al. 2021

IJMS

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Secondary metabolites isolated from plant endophytic fungi have been getting more and more attention. Some secondary metabolites exhibit high biological activities, hence, they have potential to be used for promising lead compounds in drug discovery. In this review, a total of 134 journal articles (from 2017 to 2019) were reviewed and the chemical structures of 449 new metabolites, including polyketides, terpenoids, steroids and so on, were summarized. Besides, various biological activities and structure-activity relationship of some compounds were aslo described.

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“…There are other cases related to the biosynthesis of fungi-derived compounds by Streptomyces, such as aspergilazine A (isolated from the marine-derived fungus Aspergillus taichungensis [98] and Streptomyces sp. NRRL S-1868 [99]) and violapyrone J (isolated from the fungus Cylindrocarpon [100] and Streptomyces somaliensis SCSIO ZH66 [101]). Other intriguing examples that linked the biosynthetic pathways of fungi and Streptomyces are the β-lactams cephalosporin C (isolated from the fungus Acremonium [102]) and O-carbamoyl-deacetylcephalosporin C (isolated from S. clavuligerus [103]).…”

Section: Metabolite Profile Of Promising Actinomycetes End-productsmentioning

confidence: 99%

Cliona varians-Derived Actinomycetes as Bioresources of Photoprotection-Related Bioactive End-Products

et al. 2021

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Sunscreen and sunblock are crucial skincare products to prevent photoaging and photocarcinogenesis through the addition of chemical filters to absorb or block ultraviolet (UV) radiation. However, several sunscreen and sunblock ingredients, mostly UV filters, have been associated with human and environmental safety concerns. Therefore, the exploration and discovery of promising novel sources of efficient and safer compounds with photoprotection-related activities are currently required. Marine invertebrates, particularly their associated microbiota, are promising providers of specialized metabolites with valuable biotechnological applications. Nevertheless, despite Actinobacteria members being a well-known source of bioactive metabolites, their photoprotective potential has been poorly explored so far. Hence, a set of methanolic extracts obtained from Cliona varians-derived actinomycetes was screened regarding their antioxidant and UV-absorbing capacities (i.e., photoprotection-related activities). The active extract-producing strains were identified and classified within genera Streptomyces, Micrococcus, Gordonia, and Promicromonospora. This is the first report of the isolation of these microorganisms from C. varians (an ecologically important Caribbean coral reef-boring sponge). The in vitro cytotoxicity on dermal fibroblasts of oxybenzone and the selected active extracts revealed that oxybenzone exerted a cytotoxic effect, whereas no cytotoxic effect of test extracts was observed. Accordingly, the most active (SPFi > 5, radical scavenging > 50%) and nontoxic (cell viability > 75%) extracts were obtained from Streptomyces strains. Finally, LC-MS-based characterization suggested a broad chemical space within the test strains and agreed with the reported streptomycetes’ chemodiversity. The respective metabolite profiling exposed a strain-specific metabolite occurrence, leading to the recognition of potential hits. These findings suggest that marine Streptomyces produce photoprotectants ought to be further explored in skincare applications.

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Metabolites from the endophytic fungus Cylindrocarpon sp. isolated from tropical plant Sapium ellipticum

Cited by 14 publications

References 20 publications

Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

Biological Activities of Some New Secondary Metabolites Isolated from Endophytic Fungi: A Review Study

Cliona varians-Derived Actinomycetes as Bioresources of Photoprotection-Related Bioactive End-Products

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