Metabolites Profiling of Penicillium citrinum Recovered from Endophytic of Ramie (Boehmeria nivea) as a Potential Biocontrol Against Pathogenic Fungi

Abstract

Endophytes are a potent source of bioactive compounds that mimic plant-based metabolites. Fungi Fusarium spp. and Clonostachys rosea have been identified as pathogenic microorganisms in ramie (Boehmeria nivea). The antifungal test was carried out using the diffusion method and the MIC50 and Minimum Fungicidal Concentration (MFC) values using the tested microbial pathogens were Fusarium solani isolate 3248941, F. solani isolate Colpat-359, F. oxysporum isolate N-61-2, dan Clonostachys rosea strain B3042. Identification of secondary metabolites of the extract was carried out using GC-MS. The chromatogram of GC-MS analysis of this ethyl acetate extract (EA) showed seven dominant chemical compounds with various biological activities. Ethyl asetate extract of P. citrinum showed inhibition zone ranged from 19.10–22.07 mm with strong-very strong category at 200 mg ml-1 concentration against the tested microbial pathogens. Results revealed that the significant MIC values were observed against F. solani isolate 3248941 and Clonostachys rosea strain B3042 by less than 6.3 mg ml-1 and against F. solani isolate Colpat-359 and F. oxysporum isolate N-61-2 with 12.5 mg ml-1 and 25 mg ml-1 respectively. Minimum Fungicidal concentrations (MFC) for EA of P. citrinum were also reported against F. solani isolate 3248941 and C. rosea strain B3042, by 12.5 mg ml-1, whilst Fusarium solani isolate Colpat-359 by 25 mg ml-1 and Fusarium oxysporum isolate N-61-2 ,by 50 mg ml-1 respectively. Results of this study showed that P. citrinum, endophytic fungi of ramie, could be a promising source of compounds for antifungal agents.

Identification of oil palm tissue culture contaminants by using DNA barcodes: preliminary results

“…While Tritirachium oryzae was found as an endophyte of Pinus wallichiana [26] and Syzygium malaccense [27]. Penicillium citrinum was reported as an endophyte of Boehmeria nivea [28], and Perenniporia tephropora was reported as an endophyte of Centella asiatica [29]. These preliminary results suggest recommendations to control the laboratory contamination problem.…”

Efektivitas Seed Coating Berbahan Aktif Jamur Antagonis Mengendalikan Penyakit Rebah Kecambah Pada Bibit Cabai

“…Isolat P. citrinum P3.10 merupakan jamur endofit daun dari tanaman rawa gambut yang telah dianalisis melalui analisis molekular, dimanfaatkan sebagai pupuk hayati (Hakim et al, 2019). Dilaporkan oleh Sari et al (2023) bahwa jamur P. citrinum isolat MEBP0017 berpotensi untuk dikembangkan sebagai kandidat antifungi terhadap patogen pada tanaman rami termasuk Fusarium spp. dan Clonostachys rosea strain B304.…”