Metabolites profiling of Ziziphus leaf taxa via UHPLC/PDA/ESI-MS in relation to their biological activities

Sakna, Sarah T.; Mocan, Andrei; Sultani, Haider N.; El-fiky, Nabaweya M.; Wessjohann, Ludger A.; Farag, Mohamed A.

doi:10.1016/j.foodchem.2019.04.097

Cited by 42 publications

(29 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Briefly, dried finely pulverized L. nudicaulis specimen (10 mg) was extracted by adding 2 mL 100% MeOH, containing 10 μg/mL −1 umbelliferone as an internal standard sonicated for 20 min with frequent shaking, then centrifuged at 12,000 g for 10 min to remove debris. The filtered extract through a 22-μm filter (about 500 μL) was subjected to solid-phase extraction using a C18 cartridge as previously described [114]. L. nudicaulis extract (2 μL) was loaded on HSS T3 column (100 × 1.0 mm, particle size 1.8 μm; Waters) installed on an ACQUITY UPLC system (Waters, Milford, MA, USA) equipped with a 6540 Ultra-High-Definition (UHD) Accurate-Mass Q-TOFLC/MS (Agilent, Palo Alto, CA, USA) coupled to an ESI interface, operated in positive or negative ion mode following conditions described in 113.…”

Section: High-resolution Ultra-performance Liquid Chromatography-massmentioning

confidence: 99%

Chemical Profile of Launaea nudicaulis Ethanolic Extract and Its Antidiabetic Effect in Streptozotocin-Induced Rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

Launaea nudicaulis is used in folk medicine worldwide to treat several diseases. The present study aimed to assess the antidiabetic activity of L. nudicaulis ethanolic extract and its effect on diabetic complications in streptozotocin-induced hyperglycemic rats. The extract was orally administrated at 250 and 500 mg/kg/day for 5-weeks and compared to glibenclamide as a reference drug at a dose of 5 mg/kg/day. Administration of the extract exhibited a potential hypoglycemic effect manifested by a significant depletion of serum blood glucose concurrent with a significant elevation in serum insulin secretion. After 5-weeks, extract at 250 and 500 mg/kg/day decreased blood glucose levels by about 53.8 and 68.1%, respectively, compared to the initial values (p ≤ 0.05). The extract at the two dosages prevented weight loss of rats from the 2nd week till the end of the experiment, compared to diabetic control rats. The extract further exhibited marked improvement in diabetic complications including liver, kidney and testis performance, oxidative stress, and relative weight of vital organs, with respect to diabetic control. Histopathological examinations confirmed the previous biochemical analysis, where the extract showed a protective effect on the pancreas, liver, kidney, and testis that degenerated in diabetic control rats. To characterize extract composition, UPLC-ESI–qTOF-MS identified 85 chromatographic peaks belonging to flavonoids, phenolics, acyl glycerols, nitrogenous compounds, and fatty acids, with four novel phenolics reported. The potential anti-diabetic effect warrants its inclusion in further studies and or isolation of the main bioactive agent(s).

show abstract

Section: High-resolution Ultra-performance Liquid Chromatography-massmentioning

confidence: 99%

Chemical Profile of Launaea nudicaulis Ethanolic Extract and Its Antidiabetic Effect in Streptozotocin-Induced Rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Major contributions to the antioxidant effects of food materials are due to the presence of phenolic acids. They are present in free or conjugated forms, such as flavonoids, and are a common class of secondary metabolites [ 25 ]. In this study, three phenolic acid conjugates were detected exclusively in negative ion mode ( Table 1 ) in peaks (4, 18, and 19) as monomer or dimer of methylated caffeic, ferulic, and dimethoxy cinnamic acids.…”

Section: Resultsmentioning

confidence: 99%

“…An Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Darmstadt, Germany) was used for high-resolution ESI-MS in positive and negative modes, and collision-induced dissociation (CID) spectra and conditioning of UHPLC as described previously [ 25 ]. Mass spectra, V-VIS spectra (220–600 nm), phytochemical dictionary of natural products database (CRC) literature was used to characterize metabolites.…”

Section: Methodsmentioning

confidence: 99%

Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats

et al. 2020

Self Cite

View full text Add to dashboard Cite

Lepidium sativum seeds are used traditionally to accelerate healing of bone fracture in addition to its culinary uses. This study aimed to characterize the osteoprotective effect of L. sativum in an ovariectomized rat model at two dose levels (50 and 100 mg/kg) using 17β-estradiol as a positive reference standard. Moreover, a complete metabolite profile of L. sativum via UHPLC/PDA/ESI–MS, as well as headspace solid-phase microextraction (SPME)-GC/MS is presented. Results revealed that L. sativum extract exhibited significant anti-osteoporotic actions as evidenced by mitigating the decrease in relative bone weight concurrent with improved longitudinal and perpendicular femur compression strength. Further, the extract enhanced the serum bone formation biomarkers lactate dehydrogenase (LDH) activity and osteocalcin levels. The extract also inhibited exhaustion of superoxide dismutase (SOD) as well as glutathione peroxidase (GPx) activities and accumulation of lipid peroxides in bone tissues. This is in addition to ameliorating the rise in the markers of bone resorption carboxyterminal telopeptide, type I (CTXI) and tartrate-resistant acid phosphatase (TRAP) and modulating receptor activator of nuclear factor kappa-Β ligand (RANKL)/ osteoprotegerin (OPG) expression. Metabolite characterization suggests that glucosinolates, lignans, coumarins, phenolic acids, and alkaloids mediate these anti-osteoporotic effects in a synergistic manner.

show abstract

“…Among the most important medicinal plants, the Zizyphus Spina-Christi Sidr tree appears, as it contains active substances in all parts of the tree. If it produces secondary metabolic compounds, including alkaloids, flavonoids, saponin, anti-oxidants, anti-inflammatory, anti-diabetic, and other medically important secondary compounds, especially in the leaves of the tree [3]. Also, the synthetic silver nanoparticles AgNPs nanoparticles are considered a promising generation of antibiotics, as these particles have activity against cancer cells and bacteria, as they are used against Gram-positive and Gramnegative bacteria [4] [5].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Effect Aqueous Extract and Nano-Sidr Synthetic of Leaves Ziziphus Spina-christi with Silver Nanoparticles the on Biofilm of Local Isolates of Staphylococcus. Spp

Najm

Al-kaabi

Kadhum

2021

JPRI

View full text Add to dashboard Cite

Aims: This study aims to investigate the inhibitory susceptibility of extract Sidr leaf (Ziziphus spina-christi) aqueous, silver nanoparticles Ag NPs and Sidr nanoparticle "Nano-sidr" (Ziziphus spina-christi) against local isolates of Staphylococcus.SPP, and their inhibition effect on biofilm formation. Study Design: Initially diagnosed by The use of Mannitol salt agar medium, then 26 isolates were selected from them depending on the resistance to methicilln and Vancomycin that were conducted. Place and Duration of Study: The samples were collected from AL-Najaf AL-Ashraf and Baghdad hospitals. Methodology: These isolates were subjected to a VITEK-2 compact system "ID, AST", to ascertain the genus and type of Staphylococcus bacteria., morphological and biochemical tests were conducted on them to confirm them. Results: The result of the diagnosis showed 10 isolates belonging to Staphylococcus aureus, and a number of them were resistant to as MRSA-VRSA, and 7 were Staphylococcus haemolyticus and they were all resistant and known as MRSA-VRSA, and two isolates of Staphylococcus sciuri bacteria, one of them was resistant to VRSA and the other to MRSA-VRSA , two isolates of Staphylococcus warneri were both resistant to MRSA, two isolates of Staphylococcus lugdunensis were resistant to antibiotics, one of them was VRSA and the other was MRSA-VRSA, and one isolate of Staphylococcus lentus was also resistant to antibiotics MRSA-VRSA, and one isolate of Staphylococcus warneri was resistant to MRSA-VRSA, and one isolate of Staphylococcus lugdunensis was resistant to antibiotics. Conclusion: Isolation of Staphylococcus vitulinus. 11 different isolates were selected from them according to their resistance to antibiotics, and after selecting the most efficient one by examining the inhibitory activity by diffusion method. The results showed the ability of both types of nanoparticles. Plant extracts prevent the formation of biofilms

show abstract

Metabolites profiling of Ziziphus leaf taxa via UHPLC/PDA/ESI-MS in relation to their biological activities

Cited by 42 publications

References 39 publications

Chemical Profile of Launaea nudicaulis Ethanolic Extract and Its Antidiabetic Effect in Streptozotocin-Induced Rats

Chemical Profile of Launaea nudicaulis Ethanolic Extract and Its Antidiabetic Effect in Streptozotocin-Induced Rats

Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats

Comparison of the Effect Aqueous Extract and Nano-Sidr Synthetic of Leaves Ziziphus Spina-christi with Silver Nanoparticles the on Biofilm of Local Isolates of Staphylococcus. Spp

Contact Info

Product

Resources

About