Metabolomic Analysis and Phenylpropanoid Biosynthesis in Hairy Root Culture of Tartary Buckwheat Cultivars

Thwe, Aye Aye; Kim, Jae Kwang; Li, Xiaohua; Kim, Yeon Bok; Uddin, Romij; Kim, Sun-Ju; Suzuki, Tatsuro; Park, Nam Il; Park, Sang Un

doi:10.1371/journal.pone.0065349

Cited by 38 publications

(29 citation statements)

References 41 publications

(44 reference statements)

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“…Anthocyanin was extracted and quantified according to Thwe et al . () reported method with slight modifications. Briefly, 100 mg of ground samples were placed into eppendorf tubes.…”

Section: Methodsmentioning

confidence: 99%

Effects of light on growth, levels of anthocyanin, concentration of metabolites in Fagopyrum tataricum sprout cultures

Peng

Zou

Yan-mei

et al. 2015

Int J of Food Sci Tech

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SumamyTartary buckwheat (Fagopyrum tataricum) sprouts are a popular healthy product around the world. The purpose of this study was to investigate the effects of light on growth, levels of anthocyanin, metabolite concentration in tartary buckwheat sprouts. To investigate the metabolites and accumulation of anthocyanin in tartary buckwheat sprouts, gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) were conducted. The levels of Cyanidin 3-O-glucoside and Cyanidin 3-O-rutinoside were higher under light/dark condition than that under dark condition. A total of fifty-five metabolites, including nineteen amino acids, fifteen organic acids, eight sugars, four amines, two sugar alcohols, seven other kinds were detected in tartary buckwheat sprouts. Multivariate statistical methods were used to fully distinguish between light/dark and dark condition, and eleven compounds were defined as deferentially expressed metabolites using these methods. Our results may be helpful for practical application in the production of nutritional buckwheat sprouts in the future.

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“…Anthocyanin was extracted and quantified according to Thwe et al . () reported method with slight modifications. Briefly, 100 mg of ground samples were placed into eppendorf tubes.…”

Section: Methodsmentioning

confidence: 99%

Effects of light on growth, levels of anthocyanin, concentration of metabolites in Fagopyrum tataricum sprout cultures

Peng

Zou

Yan-mei

et al. 2015

Int J of Food Sci Tech

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“…Polar metabolite extraction was performed as described previously. 24 A total of 20 mg of ground sample was extracted with 1 mL of a mixed solvent of methanol/water/chloroform (2.5:1:1, v/v/v). Ribitol solution (120 μL, 0.2 mg/mL) was added as an internal standard (IS).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Comparative Analysis of Flavonoids and Polar Metabolite Profiling of Tanno-Original and Tanno-High Rutin Buckwheat

Kim

Park

et al. 2014

J. Agric. Food Chem.

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Rutin is an important indicator for evaluating the quality of buckwheat. In this study, flavonoid biosynthesis was compared between two common cultivars (an original and a high-rutin line) of buckwheat, Fagopyrum esculentum Moench. Transcriptional levels of the main flavonoid biosynthetic genes were analyzed by real-time PCR, and main flavonoid metabolites were detected by high-performance liquid chromatography (HPLC); levels of gene expression varied among organs of the two cultivars. Significantly higher transcription levels of most flavonoid biosynthetic genes, except FeFLS1, were detected in stems of the high-rutin line than in stems of the original line. FeCHI and FeFLS2 genes also showed higher expression levels in seeds of the high-rutin cultivar. In contrast, FePAL, FeC4H, Fe4CL1, FeCHS, FeF3H, FeF3'H, FeFLS2, and FeDFR were highly detected in the roots of the original line. The HPLC results indicated 1.73-, 1.62-, and 1.77-fold higher accumulation of rutin (the primary flavonoid compound) in leaves, stems, and mature seeds of the high-rutin cultivar (24.86, 1.46, and 1.36 μg/mg, respectively) compared with the original cultivar (14.40, 0.90, and 0.77 μg/mg, respectively). A total of 46 metabolites were identified from seeds by gas chromatography-time-of-flight mass spectrometry. The metabolite profiles were subjected to principal component analysis (PCA). PCA could clearly differentiate the original and high-rutin cultivars. Our results indicate that the high-rutin cultivar could be an excellent alternative for buckwheat culture, and we provide useful information for obtaining this cultivar.

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“…Phenylpropanoids are a large group of compounds that play a vital role as secondary metabolites in vine tea. The structural genes in the phenylpropanoid biosynthetic pathway have been well studied [27][28][29][30]. In recent years, with the development of the Illumina HiSeq technique, the transcriptomic analysis has successfully been applied in a wide range of species to explore the secondary metabolic pathway [25,[31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

Cao

et al. 2020

BMC Plant Biol

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Background: Leaves of the medicinal plant Ampelopsis grossedentata, which is commonly known as vine tea, are used widely in the traditional Chinese beverage in southwest China. The leaves contain a large amount of dihydromyricetin, a compound with various biological activities. However, the transcript profiles involved in its biosynthetic pathway in this plant are unknown. Results: We conducted a transcriptome analysis of both young and old leaves of the vine tea plant using Illumina sequencing. Of the transcriptome datasets, a total of 52.47 million and 47.25 million clean reads were obtained from young and old leaves, respectively. Among 471,658 transcripts and 177,422 genes generated, 7768 differentially expressed genes were identified in leaves at these two stages of development. The phenylpropanoid biosynthetic pathway of vine tea was investigated according to the transcriptome profiling analysis. Most of the genes encoding phenylpropanoid biosynthesis enzymes were identified and found to be differentially expressed in different tissues and leaf stages of vine tea and also greatly contributed to the biosynthesis of dihydromyricetin in vine tea. Conclusions: To the best of our knowledge, this is the first formal study to explore the transcriptome of A. grossedentata. The study provides an insight into the expression patterns and differential distribution of genes related to dihydromyricetin biosynthesis in vine tea. The information may pave the way to metabolically engineering plants with higher flavonoid content.

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Metabolomic Analysis and Phenylpropanoid Biosynthesis in Hairy Root Culture of Tartary Buckwheat Cultivars

Cited by 38 publications

References 41 publications

Effects of light on growth, levels of anthocyanin, concentration of metabolites in Fagopyrum tataricum sprout cultures

Effects of light on growth, levels of anthocyanin, concentration of metabolites in Fagopyrum tataricum sprout cultures

Comparative Analysis of Flavonoids and Polar Metabolite Profiling of Tanno-Original and Tanno-High Rutin Buckwheat

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

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