Metabolomic analysis of organic acids, amino acids, and fatty acids in plasma of Hanwoo beef on a high-protein diet

Kim, Youngbae; Kim, Seon‐Ho; Oh, Songjin; Lee, Hyeon‐Seong; Ji, Moongi; Choi, Subin; Lee, Sang-Suk; Paik, Man‐Jeong

doi:10.1007/s11306-020-01737-4

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…Serum metabolomic profiling analysis of organic acids (OAs) and fatty acids (FAs) was performed by using gas chromatography–tandem mass spectrometry (GC–MS/MS) using the multiple reaction monitoring (MRM) mode, as described previously [ 40 ]. The simultaneous analysis was performed by using a Shimadzu 2010 Plus gas chromatograph equipped with a Shimadzu TQ 8040 triple quadruple mass spectrometer (Shimadzu, Kyoto, Japan) coupled with an Ultra-2 (5% phenyl–95% methylpolysiloxane bonded phase; 25 m × 0.20 mm i.d., 0.11 μm film thickness) cross-linked capillary column (Agilent Technologies, Atlanta, GA, USA).…”

Section: Methodsmentioning

confidence: 99%

Higher Concentration of Dietary Selenium, Zinc, and Copper Complex Reduces Heat Stress-Associated Oxidative Stress and Metabolic Alteration in the Blood of Holstein and Jersey Steers

Son

Kim

Islam

et al. 2022

Animals

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This study investigated the influence of high concentrations of dietary minerals on reducing heat stress (HS)-associated oxidative stress and metabolic alterations in the blood of Holstein and Jersey steers. Holstein steers and Jersey steers were separately maintained under a 3 × 3 Latin square design during the summer conditions. For each trial, the treatments included Control (Con; fed basal TMR without additional mineral supplementation), NM (NRC recommended mineral supplementation group; [basal TMR + (Se 0.1 ppm + Zn 30 ppm + Cu 10 ppm) as DM basis]), and HM (higher than NRC recommended mineral supplementation group; [basal TMR + (Se 3.5 ppm + Zn 350 ppm + Cu 28 ppm) as DM basis]). Blood samples were collected at the end of each 20-day feeding trial. In both breeds, a higher superoxide dismutase concentration (U/mL) along with lower HSP27 (μg/L) and HSP70 (μg/L) concentrations were observed in both mineral-supplemented groups compared to the Con group (p < 0.05). The HM group had significantly higher lactic acid levels in Jersey steers (p < 0.05), and tended to have higher alanine levels in Holstein steers (p = 0.051). Based on star pattern recognition analysis, the levels of succinic acid, malic acid, γ-linolenic acid, 13-methyltetradecanoic acid, and tyrosine decreased, whereas palmitoleic acid increased with increasing mineral concentrations in both breeds. Different treatment groups of both breeds were separated according to the VIP scores of the top 15 metabolites through PLS–DA analysis; however, their metabolic trend was mostly associated with the glucose homeostasis. Overall, the results suggested that supplementation with a higher-than-recommended concentration of dietary minerals rich in organic Se, as was the case in the HM group, would help to prevent HS-associated oxidative stress and metabolic alterations in Holstein and Jersey steers.

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Section: Methodsmentioning

confidence: 99%

Higher Concentration of Dietary Selenium, Zinc, and Copper Complex Reduces Heat Stress-Associated Oxidative Stress and Metabolic Alteration in the Blood of Holstein and Jersey Steers

Son

Kim

Islam

et al. 2022

Animals

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“…In Wagyu crossbred steers, Connolly et al [4] found that blood metabolites were either positively or negatively correlated with key production traits including growth rate, carcass weight, and subcutaneous and intramuscular fat, thus potentially offering biomarkers that could be used for individual steer selection for feedlot performance. Whereas published reports on the impacts of seasonal and dietary nutrient supplementation on rumen microbiota structure and metabolites of beef cattle abound [5][6][7], to our current knowledge of the published literature, there are no existing peer reviewed reports on the plasma metabolite profiles of tropical northern Australian beef cattle steers supplemented with the tropical legume Desmanthus. Our present research was intended to fill this knowledge gap.…”

Section: Introductionmentioning

confidence: 99%

Plasma Metabolites, Productive Performance and Rumen Volatile Fatty Acid Profiles of Northern Australian Bos indicus Steers Supplemented with Desmanthus and Lucerne

et al. 2021

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The hypothesis tested was that tropical steers supplemented with the Desmanthus legume and lucerne, a widely characterized temperate legume of high nutritive value, would elicit similar responses in plasma metabolite profiles, productive performance, nitrogen retention, and volatile fatty acids (VFA). The tannin-binding compound, polyethylene glycol-4000 (PEG), was added to the diets (160 g/kg Desmanthus dry matter) with the objective of further exploring nitrogen (N) utilization in the animals supplemented with Desmanthus relative to lucerne. From February to June 2020, sixteen yearling Brangus steers (average liveweight of 232 ± 6 kg) were fed a background diet of Rhodes grass (Chloris gayana) hay for 28 days, before introducing three Desmanthus cultivars (Desmanthus virgatus cv. JCU2, D. bicornutus cv. JCU4, D. leptophyllus cv. JCU7) and lucerne (Medicago sativa) at 30% dry matter intake (DMI). Relative to the backgrounding period, all supplemented steers exhibited similar growth performance. Steers supplemented with Desmanthus recorded a lower DMI and animal growth performance, but higher fecal N concentration than animals supplemented with lucerne. Among the three Desmanthus cultivars, there were no significant differences in N concentrations, VFA, and plasma metabolite profiles. The addition of PEG induced higher rumen iso-acid concentrations and fecal N excretion. However, feeding Desmanthus spp. to tropical Bos indicus steers could be a valuable means of increasing N utilization, which is attributable to the presence of tannins, and, consequently, improve animal productive performance. Since supplementation with lucerne resulted in higher liveweight, daily liveweight gains, and overall animal performance than supplementing with Desmanthus, the tested hypothesis that both supplements will elicit similar animal performance does not hold and must be rejected. Further in vivo investigation is needed to better understand the impact of tannins in Desmanthus on N utilization.

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Gas chromatography–mass spectrometry‐based quantitative method using tert‐butyldimethylsilyl derivatization for plasma levels of free amino acids and related metabolites in Japanese Black cattle

Do,

Yoshida,

Masaki

et al. 2024

Animal Science Journal

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The quantification of amino acid and related metabolite levels is important for evaluating amino acid metabolism and function in animals. However, a useful quantitative method is not enough. In this study, we developed and validated tert‐butyldimethylsilyl derivatization method using gas chromatography–mass spectrometry to quantify plasma levels of free amino acids and related metabolites in Japanese Black cattle. Of the 51 metabolites examined, 24, including 20 amino acids, one amine, and three keto acids, could be quantified. Compared with the trimethylsilyl derivatization method using gas chromatography–mass spectrometry, which has been used for untargeted metabolomic analysis, the present method had higher analytical reliability. This method is advantageous for assessing branched‐chain amino acid (BCAA) metabolism because it enables the quantification of not only BCAA levels (valine, leucine, and isoleucine) but also their bioactive metabolite keto acid levels (2‐ketoisovaleric acid, 2‐ketoisocaproic acid, and 2‐keto‐3‐methylvaleric acid) in the plasma. In addition, this method can quantify the plasma levels of not only tryptophan but also its bioactive metabolites kynurenine and serotonin. These results suggest that this quantitative method has the potential to further our understanding of amino acid metabolic processes and their functions in Japanese Black cattle.

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Metabolomic analysis of organic acids, amino acids, and fatty acids in plasma of Hanwoo beef on a high-protein diet

Cited by 5 publications

References 27 publications

Higher Concentration of Dietary Selenium, Zinc, and Copper Complex Reduces Heat Stress-Associated Oxidative Stress and Metabolic Alteration in the Blood of Holstein and Jersey Steers

Higher Concentration of Dietary Selenium, Zinc, and Copper Complex Reduces Heat Stress-Associated Oxidative Stress and Metabolic Alteration in the Blood of Holstein and Jersey Steers

Plasma Metabolites, Productive Performance and Rumen Volatile Fatty Acid Profiles of Northern Australian Bos indicus Steers Supplemented with Desmanthus and Lucerne

Gas chromatography–mass spectrometry‐based quantitative method using tert‐butyldimethylsilyl derivatization for plasma levels of free amino acids and related metabolites in Japanese Black cattle

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