Metabolomic analysis of seminal plasma in stallions belonging to two different horse breeds

Bazzano, Marilena; Laghi, Luca; Zhu, Cheng-bin; Giambattista, A. Di; Tesei, Beniamino; Laus, Fulvio

doi:10.1016/j.jevs.2020.103038

Cited by 1 publication

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“…It is interesting to notice that 2,3-butanediol is a catabolite of pyruvate, which could not be quantified in the present work due to the superimposition of its signal with some from glutamate. [ 40 ] Pyruvate is considered to be one of the major sources of energy for spermatozoa [ 41 ]. Higher concentrations of 2,3-butanediol in older individuals could therefore indicate a lower concentration of pyruvate, with detrimental effects on sperm quality.…”

Section: Discussionmentioning

confidence: 99%

Dominant Components of the Giant Panda Seminal Plasma Metabolome, Characterized by 1H-NMR Spectroscopy

Zhu

Liu

Luo

et al. 2022

Animals

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As an assisted breeding technique, artificial insemination has become the main effective practical approach in the captive breeding programs of giant panda worldwide. The composition of seminal plasma plays an important role in the success of breeding. The present work is the first attempt to characterize, by proton magnetic resonance spectroscopy (1H-NMR), the metabolome of healthy giant panda seminal plasma. A total of 35 molecules were quantified, with the concentration of 2,3-butanediol being significantly different between individuals younger than 8 years and older than 13 years, and other distinct age-related trends were highlighted by a multivariate analysis. Isopropanol’s concentration was significantly linked to estrus stages. Besides, the variations in the metabolome’s profile during storage were also evaluated. This study may serve as a reference for further research wishing to shed light on the biological mechanisms affecting giant panda sperm’s overall quality and may ultimately lead to novel approaches to giant panda artificial insemination.

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Section: Discussionmentioning

confidence: 99%