Metabolomic analysis reveals distinct profiles in the plasma and urine of rats fed a high-protein diet

Mu, Chunlong; Luo, Zhen; Zhu, Weiyun

doi:10.1007/s00726-015-1949-6

Cited by 16 publications

(7 citation statements)

References 47 publications

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“…Indeed, the stimulation of ketogenesis following an HP diet with low or high content of fat represents a way of by-passing storage of the energy, and thus prevents adiposity. Recent data about plasma metabolomic analysis of rats confirmed an increase in β-HB as a biomarker of HP intake (30) . However, there was no clear induction of key genes of ketogenesis, suggesting that this metabolic process is controlled by short-term post-transcriptional processes.…”

Section: Discussionmentioning

confidence: 98%

High dietary protein decreases fat deposition induced by high-fat and high-sucrose diet in rats

et al. 2015

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High-protein diets are known to reduce adiposity in the context of high carbohydrate and Western diets. However, few studies have investigated the specific high-protein effect on lipogenesis induced by a high-sucrose (HS) diet or fat deposition induced by high-fat feeding. We aimed to determine the effects of high protein intake on the development of fat deposition and partitioning in response to high-fat and/or HS feeding. A total of thirty adult male Wistar rats were assigned to one of the six dietary regimens with low and high protein, sucrose and fat contents for 5 weeks. Body weight (BW) and food intake were measured weekly. Oral glucose tolerance tests and meal tolerance tests were performed after 4th and 5th weeks of the regimen, respectively. At the end of the study, the rats were killed 2 h after ingestion of a calibrated meal. Blood, tissues and organs were collected for analysis of circulating metabolites and hormones, body composition and mRNA expression in the liver and adipose tissues. No changes were observed in cumulative energy intake and BW gain after 5 weeks of dietary treatment. However, high-protein diets reduced by 20 % the adiposity gain induced by HS and high-sucrose high-fat (HS-HF) diets. Gene expression and transcriptomic analysis suggested that high protein intake reduced liver capacity for lipogenesis by reducing mRNA expressions of fatty acid synthase (fasn), acetyl-CoA carboxylase a and b (Acaca and Acacb) and sterol regulatory element binding transcription factor 1c (Srebf-1c). Moreover, ketogenesis, as indicated by plasma β-hydroxybutyrate levels, was higher in HS-HF-fed mice that were also fed high protein levels. Taken together, these results suggest that high-protein diets may reduce adiposity by inhibiting lipogenesis and stimulating ketogenesis in the liver.

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Section: Discussionmentioning

confidence: 98%

High dietary protein decreases fat deposition induced by high-fat and high-sucrose diet in rats

et al. 2015

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“…We could not, however, measure this process in the present study because it would have required complex procedures to measure ketone body production, using stable isotopes to measure 13 C in aceto-acetate and/or ␤-hydroxybutyrate. There are very few data in the literature addressing the impact of HP diets on ketones bodies, although it was recently reported that plasma ␤-hydroxybutyrate increased in humans (42) and in rats fed an HP diet (26). Interestingly, this study (26) reported that 2-ketoglutarate and fumarate were positively correlated to phenylalanine, methionine, and serine.…”

Section: Discussionmentioning

confidence: 80%

“…There are very few data in the literature addressing the impact of HP diets on ketones bodies, although it was recently reported that plasma ␤-hydroxybutyrate increased in humans (42) and in rats fed an HP diet (26). Interestingly, this study (26) reported that 2-ketoglutarate and fumarate were positively correlated to phenylalanine, methionine, and serine. Pyruvate and citrate also robustly distinguished the group fed a high-protein diet.…”

Section: Discussionmentioning

confidence: 80%

Adaptation to a high-protein diet progressively increases the postprandial accumulation of carbon skeletons from dietary amino acids in rats

Stępień

Azzout‐Marniche

Even

et al. 2016

American Journal of Physiology-Regulatory, Integrative and Comp

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We aimed to determine whether oxidative pathways adapt to the overproduction of carbon skeletons resulting from the progressive activation of amino acid (AA) deamination and ureagenesis under a high-protein (HP) diet. Ninety-four male Wistar rats, of which 54 were implanted with a permanent jugular catheter, were fed a normal protein diet for 1 wk and were then switched to an HP diet for 1, 3, 6, or 14 days. On the experimental day, they were given their meal containing a mixture of 20 U-[N]-[C] AA, whose metabolic fate was followed for 4 h. Gastric emptying tended to be slower during the first 3 days of adaptation. N excretion in urine increased progressively during the first 6 days, reaching 29% of ingested protein.CO excretion was maximal, as early as the first day, and represented only 16% of the ingested proteins. Consequently, the amount of carbon skeletons remaining in the metabolic pools 4 h after the meal ingestion progressively increased to 42% of the deaminated dietary AA after 6 days of HP diet. In contrast, C enrichment of plasma glucose tended to increase from 1 to 14 days of the HP diet. We conclude that there is no oxidative adaptation in the early postprandial period to an excess of carbon skeletons resulting from AA deamination in HP diets. This leads to an increase in the postprandial accumulation of carbon skeletons throughout the adaptation to an HP diet, which can contribute to the sustainable satiating effect of this diet.

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“…As a result, metabolic correlation analysis could capture the relationships of their represented cellular processes and biological reactions associated with the stimulus. This has great potential for interpreting the results of metabolomics data and complementing our understanding of biological regulation in complex biological systems (Mu, Yang, Luo, & Zhu, ).…”

Section: Resultsmentioning

confidence: 99%

Early metabolic characterization of brain tissues after whole body radiation based on gas chromatography–mass spectrometry in a rat model

Yao

Cao

et al. 2018

Biomedical Chromatography

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Radiation-induced brain injury involves acute, early delayed and late delayed damage based on the time-course and clinical manifestations. The acute symptoms are mostly transient and reversible, whereas the late delayed radiation-induced changes are progressive and irreversible. Therefore, evaluation of the organ-specific early response to ionizing radiation exposure is necessary for improving treatment strategies and minimizing possible damage at an early stage after radiation exposure. In the current study, the gas chromatography-mass spectrometry technique based on metabolomics coupled with metabolic correlation network was applied to investigate the early metabolic characterization of rat brain tissues following irradiation. Our findings showed that the metabolic response to irradiation was not just limited to the variations of individual metabolite levels, but also accompanied by alterations of network correlations among various metabolites. Metabolite clustering indicated that energy metabolism disorder and inflammation response were induced following radiation exposure. The correlation networks revealed that the strong positive correlations of differential metabolites were highly reduced and significant negative linkages were highlighted in irradiated groups even without statistical changes in metabolic levels. Our findings provided new insights into our understanding of the radiation-induced acute brain injury mechanism and clues as to the therapy target for clinical applications.

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Metabolomic analysis reveals distinct profiles in the plasma and urine of rats fed a high-protein diet

Cited by 16 publications

References 47 publications

High dietary protein decreases fat deposition induced by high-fat and high-sucrose diet in rats

High dietary protein decreases fat deposition induced by high-fat and high-sucrose diet in rats

Adaptation to a high-protein diet progressively increases the postprandial accumulation of carbon skeletons from dietary amino acids in rats

Early metabolic characterization of brain tissues after whole body radiation based on gas chromatography–mass spectrometry in a rat model

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